Difference between revisions of "Team:UCLA/Notebook/Recombinant Expression/6 July 2015"
Line 290: | Line 290: | ||
{| class="wikitable" | {| class="wikitable" | ||
|- | |- | ||
− | ! | + | ! Mass of Cell Pellet |
− | ! | + | ! Lysozyme |
− | ! | + | ! PMSF Solution |
+ | ! Deoxycholic Acid | ||
|- | |- | ||
− | | | + | | 0.52g |
− | | | + | | 0.41mL |
− | | | + | | 0.78g |
|- | |- | ||
− | | | + | | 0.32g |
− | | | + | | 0.41 mL |
− | | | + | | 0.48g |
|} | |} |
Revision as of 23:16, 9 July 2015
Cell Lysis, continued
Calculations of Reagent Quantities Needed (accounting for inflation of cell pellet weight due to inclusion of Falcon tube mass)
Mass of Cell Pellet | Lysozyme | PMSF Solution | Deoxycholic Acid |
---|---|---|---|
0.52g | 0.41mL | 0.78g | |
0.32g | 0.41 mL | 0.48g |