Difference between revisions of "Template:Team:Groningen/CONTENT/Home/Blogs"
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|title=Biofilm ion selectivity | |title=Biofilm ion selectivity | ||
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| − | The γ-PGA molecule was | + | The γ-PGA molecule was modelled using Molecular Dynamics. Wetlab testing was performed using a Natto strain of <i>Bacillus subtilis</i>. |
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|title=Rigidity of the biofilm | |title=Rigidity of the biofilm | ||
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| − | To survive | + | To survive water flow the biofilm has to be stable and robust. Biofilm genes were overexpressed to be constantly transcribed. Furthermore stability was improved by repressing and knocking out genetic pathways resulting in cell motility and biofilm repression. |
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| − | The BBa_K823023 backbone is one of the standard backbones for bacillus for integrating in the <i>amyE</i> locus. However an extra integration locus is welcome when making a multiple mutant. Another integration | + | The BBa_K823023 backbone is one of the standard backbones for bacillus for integrating in the <i>amyE</i> locus. However, an extra integration locus is welcome when making a multiple mutant. Another integration locus is the thrC locus. To create this backbone the BBa_K823023 backbone was used, where the amyE locus parts were replaced with the thrC locus parts from the plasmid pdg1664. This resulted in a new shuttle vector for <i>B. subtilis</i>. |
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Revision as of 21:11, 18 November 2015
<img class="image" src="#"/>
Carrier on which the biofilm grows
Our biofilm was grow on a carrier material for strength and durability. Whatman paper was chosen for its great biofilm growth and low cost. Four growth methods were studied to optimize biofilm growth and strength.
<img class="image" src="#"/>
Biofilm ion selectivity
The γ-PGA molecule was modelled using Molecular Dynamics. Wetlab testing was performed using a Natto strain of Bacillus subtilis.
<img class="image" src="#"/>
Rigidity of the biofilm
To survive water flow the biofilm has to be stable and robust. Biofilm genes were overexpressed to be constantly transcribed. Furthermore stability was improved by repressing and knocking out genetic pathways resulting in cell motility and biofilm repression.
<img class="image" src="#"/>
New shuttle vector
The BBa_K823023 backbone is one of the standard backbones for bacillus for integrating in the amyE locus. However, an extra integration locus is welcome when making a multiple mutant. Another integration locus is the thrC locus. To create this backbone the BBa_K823023 backbone was used, where the amyE locus parts were replaced with the thrC locus parts from the plasmid pdg1664. This resulted in a new shuttle vector for B. subtilis.
<img class="image" src="#"/>
Human practices
When working with GMO’s it is important to know the regulations and think about future perspectives, which we did with possible future scenarios. Also it is important to talk about iGEM and your project at events, with several media, at this occasions we used our own card game.
<img class="image" src="#"/>
Future perspective
To implement our project the GMOs need to stay in the power plant. Therefore we thought about capsulating the bacteria into two carriers, antimicrobial layers and a kill switch.The ultimate goal is that the biofilm can sustain itself from nutrients in the water.