Difference between revisions of "Template:Team:Groningen/CONTENT/Home/Blogs"
| Line 42: | Line 42: | ||
|title=Future perspective | |title=Future perspective | ||
|content=<html> | |content=<html> | ||
| − | Our bacteria need to stay and survive in the power plant | + | Our bacteria need to stay and survive in the power plant by, possibly, growing between two carriers, being directed by antimicrobial layers and equipped with a kill switch. Ideally, the biofilm sustains itself by using the nutrients present in water. |
</html> | </html> | ||
}} | }} | ||
Revision as of 16:58, 19 November 2015
<img class="image" src="#"/>
Carrier on which the biofilm grows
Our biofilm was grow on a carrier material for strength and durability. Whatman paper was chosen for its great biofilm growth and low cost. Four growth methods were studied to optimize biofilm growth and strength.
<img class="image" src="#"/>
Biofilm ion selectivity
The ion selectivity for Na+ and Cl- of a membrane of negatively charged γ-PGA molecules was modelled using Molecular Dynamics. Wetlab testing was performed using B. subtilis Natto.
<img class="image" src="#"/>
Rigidity of the biofilm
To survive water flow, the biofilm has to be stable and robust. This was done by overexpressing genes involved in biofilm formation and by knocking out genes having the opposite effect.
<img class="image" src="#"/>
New shuttle vector
The BBa_K823023 backbone is one of the standard backbones for Bacillus for integrating in the amyE locus. However, an extra integration locus is welcome when making a multiple mutant. Another integration locus is the thrC locus. To create this backbone the BBa_K823023 backbone was used, where the amyE locus parts were replaced with the thrC locus parts from the plasmid pDG1664. This resulted in a new shuttle vector for B. subtilis.
<img class="image" src="#"/>
Human Practices
When working with GMOs for a new project, it is important to know the regulations surrounding genetic modification in general, and think about future perspectives for your project. In addition it is important to realise if and in to what extend you can implement your project and what other people think of this. To address this, we visited COGEM, designed an educational card game and considered several future scenarios involving GMOs and our project.
<img class="image" src="#"/>
Future perspective
Our bacteria need to stay and survive in the power plant by, possibly, growing between two carriers, being directed by antimicrobial layers and equipped with a kill switch. Ideally, the biofilm sustains itself by using the nutrients present in water.