Difference between revisions of "Team:Manchester-Graz/Safety"

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<h1>Safety</h1>
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<h3>Safe project design</h3>
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<p style="text-align:justify">The quorum sensing systems used in our expression system come from potential pathogens. <i>Pantoea stewartii</i> subsp. stewartii which originally contains the EsaR/I system is a pathogen causing Stewart’s wilt, a serious disease of corn. <i>Burkholderia cenocepacia</i>, delivering the CepI/R system, is an opportunistic pathogen for humans with cystic fibrosis or chronic granulomatous disease. So to avoid working with these strains we got the genes synthesized rather than obtaining them from the bacteria via PCR.<br>
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The enzymes used for the synthetic pathways of L-DOPA and Dopamine should not pose any safety risks. They are of eukaryotic origin and impose a heavy load on E. coli, so it is highly unlikely for them to spread by giving a selectional advantage.<br>
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For the first stages only standard laboratory strains of <i>E. coli </i>(BL 21 and TOP10) are used. Additionally the used ampicillin resistance ensures a minimal viability of the used plasmids outside the ampicillin media in our lab. The same applies to the integration of our construct into the probiotic <i>E. coli</i> Nissle 1917. The (at this point highly theoretical) integration of this modified probiotic strain into patients’ gut floras however would require a whole lot of other biosafety considerations, we don’t have to fully consider yet. However, the setup of our multidimensional expression system allows for the introduction of a suicide mechanism at a high specific bacterial density to reduce the chance of overgrowth of the natural gut flora.</p>
  
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<h3>Safe lab work</h3>
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<p>Outside the usual “good lab practice” no other safety measures were necessary for our work.</p>
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<h3>Safe shipment</h3>
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<p>We’ll see about that…</p>
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<a class="footerlinks" href="https://2015.igem.org/Team:Manchester-Graz" style="text-decoration:none;">Home</a><br>
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<a class="footerlinks" href="https://2015.igem.org/Team:Manchester-Graz/Project" style="text-decoration:none";>Project</a><br>
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<a class="footerlinks" href="https://2015.igem.org/Team:Manchester-Graz/Notebook" style="text-decoration:none">Notebook</a><br>
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<a class="footerlinks" href="https://2015.igem.org/Team:Manchester-Graz/Attributions" style="text-decoration:none">Attributions</a><br>
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<a class="footerlinks" href="https://2015.igem.org/Team:Manchester-Graz/Safety" style="text-decoration:none">Safety</a><br><br><br>
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Hello and welcome to our iGEM 2015 Wiki! <br>
 
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We are currently working hard on updating our webpage, so stay tuned and be excited!
 
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In the meantime you can like our facebook page and follow us on twitter! <br>
 
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Contact us
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iGEM Subteam Graz<br>
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TU Graz, Institute of Molecular Biotechnology<br>
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Petersgasse 14<br>
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8010 Graz<br><br>
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iGEM Subteam Manchester <br>
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University of Manchester<br>
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Manchester Institute of Biotechnology<br>
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131 Princess Street<br>
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Manchester M17DN
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&copy; iGEM Manchester-Graz
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Revision as of 08:18, 27 July 2015

iGEM Manchester Header

iGEM Manchester - Safety

Safety

Safe project design

The quorum sensing systems used in our expression system come from potential pathogens. Pantoea stewartii subsp. stewartii which originally contains the EsaR/I system is a pathogen causing Stewart’s wilt, a serious disease of corn. Burkholderia cenocepacia, delivering the CepI/R system, is an opportunistic pathogen for humans with cystic fibrosis or chronic granulomatous disease. So to avoid working with these strains we got the genes synthesized rather than obtaining them from the bacteria via PCR.
The enzymes used for the synthetic pathways of L-DOPA and Dopamine should not pose any safety risks. They are of eukaryotic origin and impose a heavy load on E. coli, so it is highly unlikely for them to spread by giving a selectional advantage.
For the first stages only standard laboratory strains of E. coli (BL 21 and TOP10) are used. Additionally the used ampicillin resistance ensures a minimal viability of the used plasmids outside the ampicillin media in our lab. The same applies to the integration of our construct into the probiotic E. coli Nissle 1917. The (at this point highly theoretical) integration of this modified probiotic strain into patients’ gut floras however would require a whole lot of other biosafety considerations, we don’t have to fully consider yet. However, the setup of our multidimensional expression system allows for the introduction of a suicide mechanism at a high specific bacterial density to reduce the chance of overgrowth of the natural gut flora.

Safe lab work

Outside the usual “good lab practice” no other safety measures were necessary for our work.

Safe shipment

We’ll see about that…