Difference between revisions of "Team:Vanderbilt/Parts/Repair Enzymes"
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<a href="https://2015.igem.org/Team:Vanderbilt/Parts/Part_Collection" class="btn btn-default">Part Collection</a> | <a href="https://2015.igem.org/Team:Vanderbilt/Parts/Part_Collection" class="btn btn-default">Part Collection</a> | ||
<a href="https://2015.igem.org/Team:Vanderbilt/Parts/Optimized_RFPs" class="btn btn-default">Optimized RFPs</a> | <a href="https://2015.igem.org/Team:Vanderbilt/Parts/Optimized_RFPs" class="btn btn-default">Optimized RFPs</a> | ||
| − | <a href="https://2015.igem.org/Team:Vanderbilt/Parts/ | + | <a href="https://2015.igem.org/Team:Vanderbilt/Parts/Stable_Circuits" class="btn btn-default">Stable Circuits</a> |
<a href="https://2015.igem.org/Team:Vanderbilt/Parts/Repair_Enzymes" class="btn btn-default chosen">Repair Enzymes</a> | <a href="https://2015.igem.org/Team:Vanderbilt/Parts/Repair_Enzymes" class="btn btn-default chosen">Repair Enzymes</a> | ||
</div> | </div> | ||
Revision as of 04:03, 20 November 2015
To build our genetically-engineered strain of bacteria with decreased susceptibility to mutation, we produced several parts for expression DNA repair enzymes. Each of these genes were themselves optimized by our software to have a minimal number of overall mutation hotspots. Included are enzymes for repairing UV-induced lesions, oxidative damage, and the enzymes necessary to construct our "incorruptible cell". Part numbers and their corresponding genes for each are listed below:
K1673201 T4 Pyrimidine Dimer Glycosylase (Mutation Optimized)
K1673202 Formamidopyrimidine DNA glycosylase (Mutation Optimized)
K1673203 S1 Nuclease (Overall Mutation Optimized)
K1673204 T7 Endonuclase (Mutation Optimized)
K1673205 T7 Endonuclease (Homology Optimized )
K1673213 IPTG Promoter Mutation Optimized S1 Nuclease
K1673214 IPTG Promoter Mutation Optimized T7 Endonuclease
K1673223 T7 Promoter Mutation Optimized S1 Nuclease
K1673224 T7 Promoter Mutation Optimized T7 Nuclease