Difference between revisions of "Template:Team:Groningen/CONTENT/EXPERIMENTS/Restriction ferritin (fr3)"
| Line 7: | Line 7: | ||
<div class="wrapper"> | <div class="wrapper"> | ||
<div class="header"> | <div class="header"> | ||
| − | |||
<div class="field fw3">Component</div> | <div class="field fw3">Component</div> | ||
<div class="field fw7">Amount</div> | <div class="field fw7">Amount</div> | ||
</div> | </div> | ||
<div class="record"> | <div class="record"> | ||
| − | + | <div class="field fw3">Ferritin</div> | |
| − | <div class="field fw3"> | + | |
<div class="field fw7">10 µL</div> | <div class="field fw7">10 µL</div> | ||
</div> | </div> | ||
<div class="record"> | <div class="record"> | ||
| − | |||
<div class="field fw3">10x buffer (2.1)</div> | <div class="field fw3">10x buffer (2.1)</div> | ||
<div class="field fw7">2 µL</div> | <div class="field fw7">2 µL</div> | ||
</div> | </div> | ||
<div class="record"> | <div class="record"> | ||
| − | |||
<div class="field fw3">PstI</div> | <div class="field fw3">PstI</div> | ||
<div class="field fw7">1 µL</div> | <div class="field fw7">1 µL</div> | ||
</div> | </div> | ||
<div class="record"> | <div class="record"> | ||
| − | |||
<div class="field fw3">EcoRI</div> | <div class="field fw3">EcoRI</div> | ||
<div class="field fw7">1 µL</div> | <div class="field fw7">1 µL</div> | ||
</div> | </div> | ||
<div class="record"> | <div class="record"> | ||
| − | |||
<div class="field fw3">\( \mathrm{H_2O}\)</div> | <div class="field fw3">\( \mathrm{H_2O}\)</div> | ||
<div class="field fw7">6 µL</div> | <div class="field fw7">6 µL</div> | ||
Latest revision as of 21:46, 21 November 2015
16:00, 31 August 2015 - 18:30, 10 August 2015
Digest ferritin in order to ligate them. Samples were digested for 2 hours at 37 °C.
Component
Amount
Ferritin
10 µL
10x buffer (2.1)
2 µL
PstI
1 µL
EcoRI
1 µL
\( \mathrm{H_2O}\)
6 µL
Components for digestion.