Difference between revisions of "Team:IIT Kharagpur/Notebook"
| Line 92: | Line 92: | ||
<li>No colony observed for luxR protein construct.</li> | <li>No colony observed for luxR protein construct.</li> | ||
<li>Plasmid isolation done for luxPR promoter.</li> | <li>Plasmid isolation done for luxPR promoter.</li> | ||
| − | <li>Gel electrophoresis done for luxPR promoter.</li> | + | <li>Gel electrophoresis done for luxPR promoter. |
| + | <p><img src="https://static.igem.org/mediawiki/2015/b/b0/Team_IIT_Kharagpur_luxPR_promoter.jpg" class="img-responsive"></p> | ||
| + | </li> | ||
| + | |||
<li>Lane 7 : 1kb marker<br> | <li>Lane 7 : 1kb marker<br> | ||
Revision as of 09:48, 13 July 2015
Content for the Notebook Page
(Log of the work done- Day to day, Project Component
Wise,
and/or any other characterization)
WEEK-1( July 6 - July 10 )
6th July 2015
- LB media prepared (500 ml), autoclaved and stored at 4 degrees.
- 250 ml of LB agar prepared and poured into the plates (chloramphenicol antibiotic used- concentration 1 µl/ml).
7th July 2015
- DNA from wells dissolved into 10 µl distilled water. DNA kit plate instructions
- Transformed 4µl of DNA into 100µl of E.Coli DH5α. transformation procedure
- 100µl of transformed cells were plated on chloramphenicol resistant plates.and left overnight to grow.
8th July 2015
- Colonies for luxPR promoter (BBa_K546003) constructs were observed. A colony was picked and inoculated in 3 ml culture for glycerol stock preparation.
- No colony observed for luxR protein (BBa_R6002) construct.
9th July 2015
- Transformation repeated for luxR protein construct. transformation procedure
- Culture prepared for plasmid isolation for luxPR promoter. Colony was picked and inoculated in 10 ml culture.
10th July 2015
- No colony observed for luxR protein construct.
- Plasmid isolation done for luxPR promoter.
- Gel electrophoresis done for luxPR promoter.
- Lane 7 : 1kb marker
Lane 8 : luxPR promoter