Difference between revisions of "Team:York/Protocols/Growth Media"

 
Line 13: Line 13:
 
</ul>
 
</ul>
  
−
<h3>Procedure</h3>
+
<h4>Procedure</h4>
 
<ol>
 
<ol>
 
<li>Use a container with at least double the volume of the liquid that you are making.</li>
 
<li>Use a container with at least double the volume of the liquid that you are making.</li>
Line 20: Line 20:
 
<li>Autoclave. </li>
 
<li>Autoclave. </li>
 
<li>Store at room temperature or +4°C.</li>
 
<li>Store at room temperature or +4°C.</li>
 +
</ol>
 +
 +
<h2>LB Agar</h2>
 +
 +
<h4>Procedure</h4>
 +
<ol>
 +
<li>follow steps to make lysogeny broth as above</li>
 +
<li>add 15g of agar powder per litre of LB</li>
 +
<li>autoclave in 200 or 250 mL aliquots in 500mL duran bottles</li>
 +
<li>add antibiotic desired to melted agar (~55°C)</li>
 +
<li>pour into petri dishes and leave to harden in asceptic fume hood</li>
 +
<li>invert, label, wrap with parafilm and store at 4°C</li>
 
</ol>
 
</ol>
  
 
</html>
 
</html>

Latest revision as of 15:08, 31 August 2015

Lysogeny Broth

Materials

  • 10g of tryptone
  • 5g of yeast extract
  • 10g of NaCl
  • 1L of Deionized Water
  • 1M NaCl
  • 1M KOH

Procedure

  1. Use a container with at least double the volume of the liquid that you are making.
  2. Dissolve 10 g tryptone, 5 g yeast extract, and 10 g NaCl in 950 mL deionized water.
  3. Adjust the pH of the medium to 7.0 using 1M NaOH or KOH and bring volume up to 1 liter.
  4. Autoclave.
  5. Store at room temperature or +4°C.

LB Agar

Procedure

  1. follow steps to make lysogeny broth as above
  2. add 15g of agar powder per litre of LB
  3. autoclave in 200 or 250 mL aliquots in 500mL duran bottles
  4. add antibiotic desired to melted agar (~55°C)
  5. pour into petri dishes and leave to harden in asceptic fume hood
  6. invert, label, wrap with parafilm and store at 4°C