Difference between revisions of "Team:EPF Lausanne/Test"
| Line 24: | Line 24: | ||
<script> | <script> | ||
| − | var skin="igem", | + | var skin="igem", |
| − | stylepath="/wiki/skins", | + | stylepath="/wiki/skins", |
| − | wgUrlProtocols="http\\:\\/\\/|https\\:\\/\\/|ftp\\:\\/\\/|irc\\:\\/\\/|gopher\\:\\/\\/|telnet\\:\\/\\/|nntp\\:\\/\\/|worldwind\\:\\/\\/|mailto\\:|news\\:|svn\\:\\/\\/", | + | wgUrlProtocols="http\\:\\/\\/|https\\:\\/\\/|ftp\\:\\/\\/|irc\\:\\/\\/|gopher\\:\\/\\/|telnet\\:\\/\\/|nntp\\:\\/\\/|worldwind\\:\\/\\/|mailto\\:|news\\:|svn\\:\\/\\/", |
| − | wgArticlePath="/$1", | + | wgArticlePath="/$1", |
| − | wgScriptPath="/wiki", | + | wgScriptPath="/wiki", |
| − | wgScriptExtension=".php", | + | wgScriptExtension=".php", |
| − | wgScript="/wiki/index.php", | + | wgScript="/wiki/index.php", |
| − | wgVariantArticlePath=false, | + | wgVariantArticlePath=false, |
| − | wgActionPaths={}, | + | wgActionPaths={}, |
| − | wgServer="https://2015.igem.org", | + | wgServer="https://2015.igem.org", |
| − | wgCanonicalNamespace="", | + | wgCanonicalNamespace="", |
| − | wgCanonicalSpecialPageName=false, | + | wgCanonicalSpecialPageName=false, |
| − | wgNamespaceNumber=0, | + | wgNamespaceNumber=0, |
| − | wgPageName="Team:EPF_Lausanne", | + | wgPageName="Team:EPF_Lausanne", |
| − | wgTitle="Team:EPF_Lausanne", | + | wgTitle="Team:EPF_Lausanne", |
| − | wgAction="view", | + | wgAction="view", |
| − | wgArticleId=8760, | + | wgArticleId=8760, |
| − | wgIsArticle=true, | + | wgIsArticle=true, |
| − | wgUserName="Awowen", | + | wgUserName="Awowen", |
| − | wgUserGroups=["*", "user", "autoconfirmed"], | + | wgUserGroups=["*", "user", "autoconfirmed"], |
| − | wgUserLanguage="en", | + | wgUserLanguage="en", |
| − | wgContentLanguage="en", | + | wgContentLanguage="en", |
| − | wgBreakFrames=false, | + | wgBreakFrames=false, |
| − | wgCurRevisionId=403233, | + | wgCurRevisionId=403233, |
| − | wgVersion="1.16.5", | + | wgVersion="1.16.5", |
| − | wgEnableAPI=true, | + | wgEnableAPI=true, |
| − | wgEnableWriteAPI=true, | + | wgEnableWriteAPI=true, |
| − | wgSeparatorTransformTable=["", ""], | + | wgSeparatorTransformTable=["", ""], |
| − | wgDigitTransformTable=["", ""], | + | wgDigitTransformTable=["", ""], |
| − | wgMainPageTitle="Main Page", | + | wgMainPageTitle="Main Page", |
| − | wgFormattedNamespaces={"-2": "Media", "-1": "Special", "0": "", "1": "Talk", "2": "User", "3": "User talk", "4": "2015.igem.org", "5": "2015.igem.org talk", "6": "File", "7": "File talk", "8": "MediaWiki", "9": "MediaWiki talk", "10": "Template", "11": "Template talk", "12": "Help", "13": "Help talk", "14": "Category", "15": "Category talk"}, | + | wgFormattedNamespaces={"-2": "Media", "-1": "Special", "0": "", "1": "Talk", "2": "User", "3": "User talk", "4": "2015.igem.org", "5": "2015.igem.org talk", "6": "File", "7": "File talk", "8": "MediaWiki", "9": "MediaWiki talk", "10": "Template", "11": "Template talk", "12": "Help", "13": "Help talk", "14": "Category", "15": "Category talk"}, |
| − | wgNamespaceIds={"media": -2, "special": -1, "": 0, "talk": 1, "user": 2, "user_talk": 3, "2015.igem.org": 4, "2015.igem.org_talk": 5, "file": 6, "file_talk": 7, "mediawiki": 8, "mediawiki_talk": 9, "template": 10, "template_talk": 11, "help": 12, "help_talk": 13, "category": 14, "category_talk": 15, "image": 6, "image_talk": 7}, | + | wgNamespaceIds={"media": -2, "special": -1, "": 0, "talk": 1, "user": 2, "user_talk": 3, "2015.igem.org": 4, "2015.igem.org_talk": 5, "file": 6, "file_talk": 7, "mediawiki": 8, "mediawiki_talk": 9, "template": 10, "template_talk": 11, "help": 12, "help_talk": 13, "category": 14, "category_talk": 15, "image": 6, "image_talk": 7}, |
| − | wgSiteName="2015.igem.org", | + | wgSiteName="2015.igem.org", |
| − | wgCategories=[], | + | wgCategories=[], |
| − | wgMWSuggestTemplate="https://2015.igem.org/wiki/api.php?action=opensearch\x26search={searchTerms}\x26namespace={namespaces}\x26suggest", | + | wgMWSuggestTemplate="https://2015.igem.org/wiki/api.php?action=opensearch\x26search={searchTerms}\x26namespace={namespaces}\x26suggest", |
| − | wgDBname="2015_igem_org", | + | wgDBname="2015_igem_org", |
| − | wgSearchNamespaces=[0], | + | wgSearchNamespaces=[0], |
| − | wgMWSuggestMessages=["with suggestions", "no suggestions"], | + | wgMWSuggestMessages=["with suggestions", "no suggestions"], |
| − | wgRestrictionEdit=[], | + | wgRestrictionEdit=[], |
| − | wgRestrictionMove=[], | + | wgRestrictionMove=[], |
| − | wgAjaxWatch={"watchMsg": "Watch", "unwatchMsg": "Unwatch", "watchingMsg": "Watching...", "unwatchingMsg": "Unwatching...", "tooltip-ca-watchMsg": "Add this page to your watchlist", "tooltip-ca-unwatchMsg": "Remove this page from your watchlist"}; | + | wgAjaxWatch={"watchMsg": "Watch", "unwatchMsg": "Unwatch", "watchingMsg": "Watching...", "unwatchingMsg": "Unwatching...", "tooltip-ca-watchMsg": "Add this page to your watchlist", "tooltip-ca-unwatchMsg": "Remove this page from your watchlist"}; |
| − | </script> | + | </script> |
<script type="text/javascript" src="/wiki/skins/common/wikibits.js?270"><!-- wikibits js --></script> | <script type="text/javascript" src="/wiki/skins/common/wikibits.js?270"><!-- wikibits js --></script> | ||
<!-- Head Scripts --> | <!-- Head Scripts --> | ||
| Line 71: | Line 71: | ||
<script src="/wiki/skins/common/ajaxwatch.js?270"></script> | <script src="/wiki/skins/common/ajaxwatch.js?270"></script> | ||
<script src="/wiki/skins/common/mwsuggest.js?270"></script> | <script src="/wiki/skins/common/mwsuggest.js?270"></script> | ||
| − | + | <script type="text/javascript" src="/wiki/index.php?title=-&action=raw&smaxage=0&gen=js&useskin=igem"><!-- site js --></script> | |
<!-- jQuery Javascript --> | <!-- jQuery Javascript --> | ||
<script type='text/javascript' src ='/common/jquery-latest.min.js'></script> | <script type='text/javascript' src ='/common/jquery-latest.min.js'></script> | ||
| Line 79: | Line 79: | ||
<link rel='stylesheet' type='text/css' href='/forum/forum_styles.css' /> | <link rel='stylesheet' type='text/css' href='/forum/forum_styles.css' /> | ||
<script type='text/javascript' src ='/forum/forum_scripts.js'></script> | <script type='text/javascript' src ='/forum/forum_scripts.js'></script> | ||
| − | |||
<style type="text/css"> | <style type="text/css"> | ||
| Line 87: | Line 86: | ||
display: none; | display: none; | ||
} | } | ||
| − | |||
| − | |||
| − | |||
#globalWrapper, #content { | #globalWrapper, #content { | ||
width: 100%; | width: 100%; | ||
| Line 99: | Line 95: | ||
font-size: 100%; | font-size: 100%; | ||
} | } | ||
| − | |||
| − | |||
| − | |||
img.resize50{ | img.resize50{ | ||
max-width:50%; | max-width:50%; | ||
| Line 107: | Line 100: | ||
} | } | ||
</style> | </style> | ||
| − | |||
| − | |||
| − | |||
<!-- | <!-- | ||
Stone Template | Stone Template | ||
| Line 125: | Line 115: | ||
<!-- Main Styles --> | <!-- Main Styles --> | ||
<link rel="stylesheet" href="https://2015.igem.org/Team:EPF_Lausanne/Test/css/templatemo-style?action=raw&ctype=text/css" type="text/css"> | <link rel="stylesheet" href="https://2015.igem.org/Team:EPF_Lausanne/Test/css/templatemo-style?action=raw&ctype=text/css" type="text/css"> | ||
| + | |||
| + | <!-- Footer --> | ||
| + | <link rel="stylesheet" href="https://2015.igem.org/Team:EPF_Lausanne/css/footer?action=raw&ctype=text/css" type="text/css"> | ||
<script src="https://2015.igem.org/Team:EPF_Lausanne/Test/js/mordernizr-2.6.2?action=raw&ctype=text/js"></script> | <script src="https://2015.igem.org/Team:EPF_Lausanne/Test/js/mordernizr-2.6.2?action=raw&ctype=text/js"></script> | ||
| Line 135: | Line 128: | ||
<!-- HEADER --> | <!-- HEADER --> | ||
| + | |||
<div class="site-header"> | <div class="site-header"> | ||
<div class="container"> | <div class="container"> | ||
| Line 144: | Line 138: | ||
<i class="fa fa-bars"></i> | <i class="fa fa-bars"></i> | ||
</button> | </button> | ||
| − | <a class="navbar-brand" href="https://2015.igem.org/Team:EPF_Lausanne | + | <a class="navbar-brand" href="https://2015.igem.org/Team:EPF_Lausanne">Bio Logic</a> |
</div> | </div> | ||
<div class="collapse navbar-collapse" id="main-menu"> | <div class="collapse navbar-collapse" id="main-menu"> | ||
<ul class="nav navbar-nav navbar-right"> | <ul class="nav navbar-nav navbar-right"> | ||
| − | <li><a href="https://2015.igem.org/Team:EPF_Lausanne | + | <li><a href="https://2015.igem.org/Team:EPF_Lausanne">Home</a></li> |
| − | <li><a href="https://2015.igem.org/Team:EPF_Lausanne/Project"> | + | <li class="dropdown"> |
| − | + | <a href="#" class="dropdown-toggle" data-toggle="dropdown" role="button" aria-haspopup="true" aria-expanded="false">Project <span class="caret"></span></a> | |
| − | <li><a href=" | + | <ul class="dropdown-menu"> |
| − | <li><a href=" | + | <li><a href="https://2015.igem.org/Team:EPF_Lausanne/Project/Overview">Overview</a></li> |
| − | <li><a href=" | + | <li><a href="#">Organisms</a></li> |
| + | <li><a href="#">Applications</a></li> | ||
| + | <li><a href="#">Modelling</a></li> | ||
| + | </ul> | ||
| + | </li> | ||
| + | <li class="dropdown"> | ||
| + | <a href="#" class="dropdown-toggle" data-toggle="dropdown" role="button" aria-haspopup="true" aria-expanded="false">Achievements <span class="caret"></span></a> | ||
| + | <ul class="dropdown-menu"> | ||
| + | <li><a href="#">Results</a></li> | ||
| + | <li><a href="#">Data</a></li> | ||
| + | <li><a href="#">Judging</a></li> | ||
| + | <li><a href="#">Parts</a></li> | ||
| + | </ul> | ||
| + | </li> | ||
| + | <li class="dropdown"> | ||
| + | <a href="#" class="dropdown-toggle" data-toggle="dropdown" role="button" aria-haspopup="true" aria-expanded="false">Policy and Practices <span class="caret"></span></a> | ||
| + | <ul class="dropdown-menu"> | ||
| + | <li><a href="#">Human Practices</a></li> | ||
| + | <li><a href="#">Safety</a></li> | ||
| + | </ul> | ||
| + | </li> | ||
| + | <li class="dropdown"> | ||
| + | <a href="#" class="dropdown-toggle" data-toggle="dropdown" role="button" aria-haspopup="true" aria-expanded="false">Notebook <span class="caret"></span></a> | ||
| + | <ul class="dropdown-menu"> | ||
| + | <li><a href="#">Yeast</a></li> | ||
| + | <li><a href="#">E.coli</a></li> | ||
| + | <li><a href="#">Protocols</a></li> | ||
| + | </ul> | ||
| + | </li> | ||
| + | <li class="dropdown"> | ||
| + | <a href="#" class="dropdown-toggle" data-toggle="dropdown" role="button" aria-haspopup="true" aria-expanded="false">Team <span class="caret"></span></a> | ||
| + | <ul class="dropdown-menu"> | ||
| + | <li><a href="#">Meet us</a></li> | ||
| + | <li><a href="#">Attributions</a></li> | ||
| + | <li><a href="#">Aknowledgements</a></li> | ||
| + | <li><a href="#">Timeline</a></li> | ||
| + | </ul> | ||
| + | </li> | ||
</ul> | </ul> | ||
</div> | </div> | ||
| Line 272: | Line 303: | ||
| − | <footer class=" | + | <footer class="footer-distributed"> |
| − | + | ||
| − | <div class=" | + | <div class="footer-left"> |
| − | + | ||
| − | + | <img class="resize50" src="https://static.igem.org/mediawiki/2015/5/59/Logo_iGEM_w.png" alt=""> | |
| − | + | ||
| − | + | </div> | |
| − | + | ||
| − | + | <div class="footer-center"> | |
| − | + | ||
| + | <div> | ||
| + | <i class="fa fa-map-marker"></i> | ||
| + | <p><span>Route Cantonale</span> Lausanne, Suisse</p> | ||
</div> | </div> | ||
| + | |||
| + | <div> | ||
| + | <i class="fa fa-envelope"></i> | ||
| + | <p><a href="mailto:iGEM2015dreamteam@groupes.epfl.ch">Contact us</a></p> | ||
| + | </div> | ||
| + | |||
</div> | </div> | ||
| − | + | ||
| − | + | <div class="footer-right"> | |
| + | |||
| + | <p class="footer-company-about"> | ||
| + | <span>About us</span> | ||
| + | Team of a dozen biotechnology students that will attempt to change the world | ||
| + | </p> | ||
| + | |||
| + | <div class="footer-icons"> | ||
| + | |||
| + | <a href="https://www.facebook.com/pages/IGEM-EPFL-2015/1598472927106907"><i class="fa fa-facebook"></i></a> | ||
| + | <a href="https://instagram.com/igem2015epfl/"><i class="fa fa-instagram"></i></a> | ||
| + | </div> | ||
| + | |||
| + | </div> | ||
| + | |||
| + | </footer> | ||
<script src="https://2015.igem.org/Team:EPF_Lausanne/Test/js/jquery?action=raw&ctype=text/js"></script> | <script src="https://2015.igem.org/Team:EPF_Lausanne/Test/js/jquery?action=raw&ctype=text/js"></script> | ||
Revision as of 14:32, 14 July 2015
Logic Orthogonal GRNA Implemented Circuit
The aim of our project is to enable the simple and reproducible design of modular digital circuits within living cells. Cells possess the ability to accept biological data as input and process it according to predefined instructions. We plan to harness this potential by designing bio-elements that behave as transistors. Our goal is to assemble these transistors to create a programmable logic gate array.
Thinking Binary
Boolean Logic is the bedrock of the digital revolution. Developed by George Boole in the mid-19th century, it is based on a simple set of values: 0 (“FALSE”) or 1 (“TRUE”). In computers, all information is represented by strings of 0s and 1s - multiple representations of binary digits or “Bits”. Bits are physically implemented by two-state devices named transistors. The assembling of transistors forms logic gates - which enables digital circuits to exhibit incredibly complex behaviours in the everyday objects that surround us.
A Biological Computer
Creating customizable logic gates in living cells holds the promise of revolutionizing our ability to dictate the behaviour of organism and the way they react to distinct molecular cues. This involves designing logic circuits capable of linking the many genetic regulatory networks responsible for biologic operations. An ideal genetic logic device should therefore be modular and reusable, enabling scientists to implement any complex logic functions in multiple biological systems. Our goal this summer is to achieve this by using gRNA-dCas9 combinations as the biological equivalent of the wires that connect the different components of electric circuits.
Cas9 (CRISPR associated protein 9) is an RNA-guided DNA endonuclease enzyme that can target and cleave any DNA sequence complementary to its guide RNA (gRNA). Our logic gates will be based upon a derivative of this technology, the catalytically “dead” Cas9 (dCas9) that lacks the ability to cleave DNA. dCas9’s has the very interesting property of acting as programmable transcription regulator. It it can act either as repressor by preventing the binding of the RNA polymerase (RNAP) to the targeted DNA or as an activator when fused to a polymerase recruiting element (the omega subunit of RNAP in E. Coli and VP64 in Yeast).
The bio part
To create a modular system based on these elements, we will design two different plasmids: a gate array and a linker. The gate array will contain a predefined set of logic gates. The code linking the different gates will be written on the customizeable linker plasmid.
With this project, we hope to help create robust biologically based digital devices by successfully implementing modular orthogonal logic gates in living organisms. The applications of logic circuits implemented in a biological context are vast and range from the creation of smart cells able to monitor their environment for external stimuli to new forms of cellular therapeutics with improved in vivo targeting and curing.