Difference between revisions of "Team:Chalmers-Gothenburg"
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<p id="pin">The manufacturing process of biological products is complex and requires the use of living cells. Great progress has been made with industrial production techniques but contaminations are still a considerable problem the industry faces. Insufficient control of contaminations in bioreactors could compromise entire batches, resulting in high expenses. A contamination could lead to facilities or equipment having to be shut down for lengthy periods of time in order to conduct investigations and sterilize reactors. Ensuring that the bioreactors only contain the desired producing organism is critical to facility productivity, bioreactor throughput and product quality.</p> | <p id="pin">The manufacturing process of biological products is complex and requires the use of living cells. Great progress has been made with industrial production techniques but contaminations are still a considerable problem the industry faces. Insufficient control of contaminations in bioreactors could compromise entire batches, resulting in high expenses. A contamination could lead to facilities or equipment having to be shut down for lengthy periods of time in order to conduct investigations and sterilize reactors. Ensuring that the bioreactors only contain the desired producing organism is critical to facility productivity, bioreactor throughput and product quality.</p> | ||
| − | <p id="pin"> We, Team Chalmers Gothenburg, have developed a novel strategy to detect and combat contaminations in continuous bioreactors, using <i>Saccharomyces cerevisiae</i> as the producing organism.</p> | + | <p id="pin"> We, <b>Team Chalmers Gothenburg</b>, have developed a novel strategy to detect and combat contaminations in continuous bioreactors, using <i>Saccharomyces cerevisiae</i> as the producing organism.</p> |
| − | <p id="pin">The method for detection utilizes the pheromone pathway in <i>S. cerevisiae</i> where the GCP-receptor (Ste2) has been replaced with a fusion receptor, allowing the cells to detect ligands from contaminants. When a ligand binds to the fusion receptor it will activate a signal cascade within the cell, inducing an expression of red fluorescent proteins that can be observed externally. The method for combating the detected contaminant encompasses the use of radiation, which effectively harms all living organisms. In order to prevent the producing cells from becoming inviable from the irradiation treatment, a DNA-repair system from the bacterium <i>Deinococcus radiodurans</i> is implemented into the cells. <i>D. radiodurans</i> is renowned for its extreme resistance to radiation, and our theory is that implementing these enzymes may increase <i>S. cerevisiae</i>'s resistance to radiation, allowing it to survive while the contaminant dies.</p> | + | <p id="pin">The method for detection utilizes the pheromone pathway in <i>S. cerevisiae</i> where the GCP-receptor (Ste2) has been replaced with a fusion receptor, allowing the cells to detect ligands from contaminants. When a ligand binds to the fusion receptor it will activate a signal cascade within the cell, inducing an expression of <hlk style="color: #FF2400 !important;"> red fluorescent proteins </hlk> that can be observed externally. The method for combating the detected contaminant encompasses the use of radiation, which effectively harms all living organisms. In order to prevent the producing cells from becoming inviable from the irradiation treatment, a DNA-repair system from the bacterium <i>Deinococcus radiodurans</i> is implemented into the cells. <i>D. radiodurans</i> is renowned for its extreme resistance to radiation, and our theory is that implementing these enzymes may increase <i>S. cerevisiae</i>'s resistance to radiation, allowing it to survive while the contaminant dies.</p> |
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| − | <a href="http://www.chalmers.se/en/about-chalmers/alumni/chalmersmastercard/Pages/default.aspx"><img src="https://static.igem.org/mediawiki/2015/b/b6/Mastercard_logo.png" alt="Promega logo" width=" | + | <a style="margin-top: -20px;" href="http://sysbio.se/"><img src="https://static.igem.org/mediawiki/2015/5/5c/Sysbio_logo.png" alt="Sysbio logo" width="160" align="middle"></a> |
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| + | <a href="http://www.chalmers.se/en/about-chalmers/alumni/chalmersmastercard/Pages/default.aspx"><img src="https://static.igem.org/mediawiki/2015/b/b6/Mastercard_logo.png" alt="Promega logo" width="180" align="middle"></a> | ||
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| − | <a style="margin: 5px 50px;" href="http://www.promega.com"><img style='margin:20px;'src="https://static.igem.org/mediawiki/2015/thumb/0/03/Promega-Logo_blue.png/800px-Promega-Logo_blue.png" alt="Promega logo" width=" | + | <a style="margin: 5px 50px;" href="http://www.promega.com"><img style='margin:20px;'src="https://static.igem.org/mediawiki/2015/thumb/0/03/Promega-Logo_blue.png/800px-Promega-Logo_blue.png" alt="Promega logo" width="180" align="middle"></a> |
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Revision as of 13:05, 14 July 2015
A study in Scarlet
The manufacturing process of biological products is complex and requires the use of living cells. Great progress has been made with industrial production techniques but contaminations are still a considerable problem the industry faces. Insufficient control of contaminations in bioreactors could compromise entire batches, resulting in high expenses. A contamination could lead to facilities or equipment having to be shut down for lengthy periods of time in order to conduct investigations and sterilize reactors. Ensuring that the bioreactors only contain the desired producing organism is critical to facility productivity, bioreactor throughput and product quality.
We, Team Chalmers Gothenburg, have developed a novel strategy to detect and combat contaminations in continuous bioreactors, using Saccharomyces cerevisiae as the producing organism.
The method for detection utilizes the pheromone pathway in S. cerevisiae where the GCP-receptor (Ste2) has been replaced with a fusion receptor, allowing the cells to detect ligands from contaminants. When a ligand binds to the fusion receptor it will activate a signal cascade within the cell, inducing an expression of
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