Difference between revisions of "Team:NUDT CHINA"

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<h4>You can know about us:  </h4>
 
<h4>You can know about us:  </h4>
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<p> Project Description:</p>
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<li>  <a href="https://2015.igem.org/Team:NUDT_CHINA/Description">Project Description</a> </li>
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<li>  <a href="https://2015.igem.org/Requirements">Requirements page </a> </li>
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<li> <a href="https://2015.igem.org/Wiki_How-To">Wiki Requirements page</a></li>
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<h4> Styling your wiki </h4>
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<h4> Project Description </h4>
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<p>You may style this page as you like or you can simply leave the style as it is. You can easily keep the styling and edit the content of these default wiki pages with your project information and completely fulfill the requirement to document your project.</p>
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<p>For years, prokaryotic cells have been widely applied in synthetic biology and bio-engineering as the host organism. However, lacking of the compartmentation of the heterologous metabolic pathways,  which results in a relevantly low concentration of substrate and enzyme, may cause a low production or efficacy of the product, especially when producing through a complex multi-enzymatic cascade. In the current study, we developed a new method to accelerate a multi-enzymatic reaction by integration of a TALE-based scaffold system into the bacteria chassis. In this system, we designed two different random DNA sequences as binding motifs, and then incorporated them into the plasmid. Meanwhile, the different TALE proteins, which could specifically target the corresponding binding motifs, were generated by the assembly of repeat-variable di-residue (RVD) part coding sequences. Subsequently, multi-enzymes were fused with these TALE proteins, which could efficiently guide the enzymes around the DNA scaffolds, enrich the local enzyme concentration, and promote the rate of reaction. The effect of the TALE-based scaffold system were tested by the fragmented GFP study and validated by the heterologous production of plant IAA through incorporating the TALE-fused IAAM and IAAH enzymes in E.coli. To the best of our knowledge, this technique might provide a powerful way in synthesizing multi-enzymatic reaction programs in prokaryotic chassis for a wide range of application.</p>  
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<p>While you may not win Best Wiki with this styling, your team is still eligible for all other awards. This default wiki meets the requirements, it improves navigability and ease of use for visitors, and you should not feel it is necessary to style beyond what has been provided.</p>  
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<h4> Editing your wiki </h4>
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<li>  <a href="https://2015.igem.org/Team:NUDT_CHINA/Description">Coming Soon~</a> </li>
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<p>On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world! </p>
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<p> <a href="https://2015.igem.org/wiki/index.php?title=Team:NUDT_CHINA&action=edit"> Click here to edit this page! </a></p>
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<p>See tips on how to edit your wiki on the <a href="https://2015.igem.org/TemplatesforTeams_Code_Documentation">Template Documentation</a> page.</p>
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<h4>Templates </h4>
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<p> This year we have created templates for teams to use freely. More information on how to use and edit the templates can be found on the
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<a href="https://2015.igem.org/TemplatesforTeams_Code_Documentation">Template Documentation </a> page.</p>
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<h4>Tips</h4>
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<p>This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started: </p>
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<li>State your accomplishments! Tell people what you have achieved from the start. </li>
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<li>Be clear about what you are doing and how you plan to do this.</li>
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<li>You have a global audience! Consider the different backgrounds that your users come from.</li>
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<li>Make sure information is easy to find; nothing should be more than 3 clicks away. </li>
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<li>Avoid using very small fonts and low contrast colors; information should be easy to read.  </li>
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<li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="https://2015.igem.org/Calendar_of_Events">iGEM 2015 calendar</a> </li>
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<li>Have lots of fun! </li>
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<h4>Inspiration</h4>
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<p> You can also view other team wikis for inspiration! Here are some examples:</p>
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<ul>
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<li> <a href="https://2014.igem.org/Team:SDU-Denmark/"> 2014 SDU Denmark </a> </li>
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<li> <a href="https://2014.igem.org/Team:Aalto-Helsinki">2014 Aalto-Helsinki</a> </li>
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<li> <a href="https://2014.igem.org/Team:LMU-Munich">2014 LMU-Munich</a> </li>
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<li> <a href="https://2014.igem.org/Team:Michigan"> 2014 Michigan</a></li>
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<li> <a href="https://2014.igem.org/Team:ITESM-Guadalajara">2014 ITESM-Guadalajara </a></li>
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<li> <a href="https://2014.igem.org/Team:SCU-China"> 2014 SCU-China </a></li>
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<h4> Uploading pictures and files </h4>
 
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<p> You can upload your pictures and files to the iGEM 2015 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name. <br />
 
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When you upload, set the "Destination Filename" to <code>Team:YourOfficialTeamName/NameOfFile.jpg</code>. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)</p>
 
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<a href="https://2015.igem.org/Special:Upload">CLICK HERE TO UPLOAD FILES</a>
 
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Revision as of 13:19, 14 July 2015

Welcome to iGEM 2015!

Nice to meet you! We are Team_NUDT_CHINA.

You can know about us:

  • Project Description

    • Project Description

    For years, prokaryotic cells have been widely applied in synthetic biology and bio-engineering as the host organism. However, lacking of the compartmentation of the heterologous metabolic pathways,  which results in a relevantly low concentration of substrate and enzyme, may cause a low production or efficacy of the product, especially when producing through a complex multi-enzymatic cascade. In the current study, we developed a new method to accelerate a multi-enzymatic reaction by integration of a TALE-based scaffold system into the bacteria chassis. In this system, we designed two different random DNA sequences as binding motifs, and then incorporated them into the plasmid. Meanwhile, the different TALE proteins, which could specifically target the corresponding binding motifs, were generated by the assembly of repeat-variable di-residue (RVD) part coding sequences. Subsequently, multi-enzymes were fused with these TALE proteins, which could efficiently guide the enzymes around the DNA scaffolds, enrich the local enzyme concentration, and promote the rate of reaction. The effect of the TALE-based scaffold system were tested by the fragmented GFP study and validated by the heterologous production of plant IAA through incorporating the TALE-fused IAAM and IAAH enzymes in E.coli. To the best of our knowledge, this technique might provide a powerful way in synthesizing multi-enzymatic reaction programs in prokaryotic chassis for a wide range of application.

  • Coming Soon~