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| − | <!-- Start of CSS-->
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| − | <style type="text/css">
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| − | /* PAGE LAYOUT */
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| − | /* Change Background color*/
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| − | body {
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| − | background-color: #fff;
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| − | }
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| − | /* Creates a container that will wrap all of the content inside your wiki pages. */
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| − | #mainContainer {
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| − | width: 978px;
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| − | overflow:hidden;
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| − | float:left;
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| − | margin-left:20px;
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| − | margin-bottom: 10px;
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| − | background-color: #fff;
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| − | border-bottom: 14px solid #565656;
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| − | border-right: 2px solid #565656;
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| − | border-left: 2px solid #565656;
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| − | border-top: 2px solid #565656;
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| − | font-family: "Trebuchet MS", Helvetica, sans-serif;
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| − | }
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| − | /* Creates the container for the menu */
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| − | #menuContainer {
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| − | float:left;
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| − | width: 134px;
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| − | padding: 20px 0px;
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| − | border-top: 14px solid #565656;
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| − | background-color: #E8E8E9;
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| − | }
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| − |
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| − | /* Creates the container for the content */
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| − | #contentContainer {
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| − | padding-top:20px;
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| − | padding-right:10px;
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| − | margin-bottom: 20px;
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| − | width: 814px;
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| − | padding-left: 20px;
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| − | float: left;
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| − | background-color: #fff;
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| − | border-top: 14px solid #565656;
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| − | font-family: "Trebuchet MS", Helvetica, sans-serif;
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| − | }
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| − | /*Set up height place holder for the banner*/
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| − | #bannerContainer {
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| − | height:200px;
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| − | margin:auto;
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| − | text-align:center;
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| − | color: #24B694;
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| − | }
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| − |
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| − | /*VISUAL STYLE (TABLES, FONTS, LINKS, IMAGES, UL) */
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| − | /*Change the styling of text for everything inside main container*/
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| − | #mainContainer p {
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| − | font-size: 13px;
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| − | color: #000000;
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| − | }
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| − |
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| − | /*This changes the color and font family */
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| − | #contentContainer h1, h2, h3, h4, h5, h6 {
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| − | color: #565656;
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| − | border-bottom: none;
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| − | font-weight: bold;
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| − | font-family: "Trebuchet MS", Helvetica, sans-serif;
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| − | margin-top:10px;
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| − | }
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| − | /*Style of the links - links are different inside the menu */
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| − | #contentContainer a {
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| − | font-weight: bold;
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| − | color: #23b593;
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| − | }
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| − |
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| − | /* Styling links on hover- links are different inside the menu */
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| − | #contentContainer a:hover {
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| − | color: #59bf92;
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| − | }
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| − |
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| − | /*Change the styling of tables */
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| − | #contentContainer table {
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| − | border: 1px solid #565656;
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| − | border-collapse: collapse;
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| − | width: 90%
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| − | margin: auto;
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| − | margin-bottom: 15px;
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| − | margin-top: 15px;
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| − | margin-right: 10px;
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| − | margin-left: 10px;
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| − | }
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| − | /*Change the styling of table cells*/
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| − | #contentContainer td {
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| − | padding: 10px;
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| − | border: 1px solid #565656;
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| − | border-collapse: collapse;
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| − | vertical-align: text-top;
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| − | }
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| − | /*Change the styling of table headers */
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| − | #contentContainer th {
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| − | background-color: #E8E8E9;
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| − | padding: 10px;
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| − | border: 1px solid #565656;
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| − | border-collapse: collapse;
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| − | vertical-align: text-top;
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| − | }
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| − | /*MENU STYLING */
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| − | /*Styling for the links in the menu */
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| − | #menuContainer a {
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| − | color: #565656;
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| − | text-decoration:none;
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| − | font-weight: bold;
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| − | }
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| − | /* Sets the style for lists inside menuContainer */
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| − | #menuContainer ul {
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| − | list-style: none;
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| − | margin-left:0px;
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| − | }
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| − | /*Styles the list items to become menu buttons */
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| − | #menuContainer ul li {
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| − | text-align: center;
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| − | display: block;
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| − | width: 100%;
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| − | height:30px;
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| − | padding-top:10px;
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| − | }
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| − | /*For the menu buttons, changes the color when hovering*/
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| − | #menuContainer li:hover {
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| − | color: #FFF;
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| − | background-color: #24B694;
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| − | }
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| − | /*Submenus are not displayed as default*/
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| − | #menuContainer li ul {
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| − | display: none;
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| − | padding-top:15px;
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| − | margin-left: -19px;
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| − | }
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| − | /*Submenus are displayed when hovering the menu button */
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| − | #menuContainer li:hover ul {
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| − | /*display: inline-block; */
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| − | display: block;
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| − | position: absolute;
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| − | float:right;
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| − | margin-left: 134px;
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| − | margin-top:-42px;
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| − | }
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| − |
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| − | /*Style the submenu buttons*/
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| − | #menuContainer li ul li{
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| − | background-color: #59BF92;
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| − | padding-left:20px;
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| − | padding-right:20px;
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| − | height:30px;
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| − | padding-top:10px;
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| − | margin-top:-2px;
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| − | color: #565656;
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| − | width: 150px;
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| − | }
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| − |
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| − | /*CLASSES */
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| − | /*Clear class for all the pages, adds spacing too*/
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| − | .clear{
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| − | clear:both;
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| − | height: 10px;
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| − | }
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| − | /* highlight box for special messages */
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| − | .highlightBox {
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| − | width:500px;
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| − | margin:auto;
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| − | background-color: #E8E8E9;
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| − | margin-bottom: 15px;
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| − | margin-top: 15px;
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| − | padding: 15px;
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| − | padding-top: 5px;
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| − | }
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| − | </style>
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| − | <!-- End of CSS -->
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| − |
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| − | <!-- Start of the template html elements. -->
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| − | <div id ="mainContainer"><!--The closing tag for mainContainer should be placed at the bottom of each content page.-->
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| − | <div id="bannerContainer">
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| − | <br><br>
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| − | <h2> Add a banner to your wiki! </h2>
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| − | <p>You can make the image 980px by 200px</p>
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| − | <p> Remember to call the file: "<i>Team_NTU-LIHPAO-Taiwan_banner.jpg</i>" </p>
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| − | </div>
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| − | <!-- Start of menu -->
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| − | <div id="menuContainer">
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| − | <!-- This list is your menu, every list item is a menu button and nested listed become submenu buttons -->
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| − | <ul>
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| − | <a href="https://2015.igem.org/Team:NTU-LIHPAO-Taiwan"><li>HOME</li></a>
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| − | <a href="https://2015.igem.org/Team:NTU-LIHPAO-Taiwan/Team"><li>TEAM</li></a>
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| − | <a href="#"><li>PROJECT
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| − | <ul>
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| − | <a href="https://2015.igem.org/Team:NTU-LIHPAO-Taiwan/Abstract"><li>Abstract</li></a>
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| − | <a href="https://2015.igem.org/Team:NTU-LIHPAO-Taiwan/Description"><li>Description</li></a>
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| − | <a href="https://2015.igem.org/Team:NTU-LIHPAO-Taiwan/Experiments"><li>Experiments & Protocols</li></a>
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| − | <a href="https://2015.igem.org/Team:NTU-LIHPAO-Taiwan/Results"><li>Results</li></a>
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| − | <a href="https://2015.igem.org/Team:NTU-LIHPAO-Taiwan/Design"><li>Design</li></a>
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| − | </ul>
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| − | </li></a>
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| − | <a href="#"><li>PARTS
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| − | <ul>
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| − | <a href="https://2015.igem.org/Team:NTU-LIHPAO-Taiwan/Parts"><li>Team Parts</li></a>
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| − | <a href="https://2015.igem.org/Team:NTU-LIHPAO-Taiwan/Basic_Part"><li>Basic Parts</li></a>
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| − | <a href="https://2015.igem.org/Team:NTU-LIHPAO-Taiwan/Composite_Part"><li>Composite Parts</li></a>
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| − | <a href="https://2015.igem.org/Team:NTU-LIHPAO-Taiwan/Part_Collection"><li>Part Collection</li></a>
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| − | </ul>
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| − | </li></a>
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| − | <a href="https://2015.igem.org/Team:NTU-LIHPAO-Taiwan/Notebook"><li>NOTEBOOK</li></a>
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| − | <a href="https://2015.igem.org/Team:NTU-LIHPAO-Taiwan/Attributions"><li>ATTRIBUTIONS</li></a>
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| − | <a href="https://2015.igem.org/Team:NTU-LIHPAO-Taiwan/Collaborations"><li>COLLABORATIONS</li></a>
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| − | <a href="https://2015.igem.org/Team:NTU-LIHPAO-Taiwan/Practices"><li>HUMAN PRACTICES</li></a>
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| − | <a href="https://2015.igem.org/Team:NTU-LIHPAO-Taiwan/Safety"><li>SAFETY</li></a>
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| − | <a href="https://2015.igem.org/Team:NTU-LIHPAO-Taiwan/Modeling"><li>MODELING</li></a>
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| − | <a href="https://2015.igem.org/Team:NTU-LIHPAO-Taiwan/Measurement"><li>MEASUREMENT</li></a>
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| − | <a href="https://2015.igem.org/Team:NTU-LIHPAO-Taiwan/Software"><li>SOFTWARE</li></a>
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| − | <a href="https://2015.igem.org/Team:NTU-LIHPAO-Taiwan/Entrepreneurship"><li>ENTREPRENEURSHIP</li></a>
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| − | </ul>
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| − | </div>
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| − | <!-- End of menu -->
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| − |
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| − | <!-- Start of content -->
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| − | <div id="contentContainer"> <!--The closing tag for contentContainer should be placed at the bottom of each content page.-->
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| | <h1>CPP-PYY complex for appetite suppression</h1> | | <h1>CPP-PYY complex for appetite suppression</h1> |
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Cell penetrating peptide (CPP) is a short peptide that is able to bring DNA or protein as cargo for spontaneous penetration through cell membrane, characterized by two mechanisms: Direct penetration and Endocytosis. They share 3 steps in common: membrane interaction, membrane permeation, and CPPs releasing into cytosol.
Our project design devotes to combining CPP and PYY, with Lactobacillus. casei as a vector, for constructing a dietary supplement for appetite suppression. The CPP-PYY complex secreted from L. casei can effectively pass through the intestine to increase PYY circulation, thus results in appetite inhibition and decrease in food intake, which serves as a cure for obesity.
Experiments:
There parts are being tested in E. coli for initial examination:
1. CPP-PYY complex production
2. Nisin selection
3. Suicide device for safety