Difference between revisions of "Team:Paris Saclay/Notebook/July/9"

(Thursday 9th July)
(Quantification)
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====Quantification====
 
====Quantification====
 
''by Pauline and Audrey''
 
''by Pauline and Audrey''
 +
 +
Preparation of Agarose Gel 1%, 0,5g in 50mL of 1X TAE, 0,5µL of BET
 +
Migration 0,06A 80V
 
We quantify with the electrophoresis gel:
 
We quantify with the electrophoresis gel:
 
* C0040: 10ng/µL
 
* C0040: 10ng/µL

Revision as of 09:39, 17 July 2015

Thursday 9th July

Lab Work

Transformation

by Coralie

Ligation product:

  • J23101 + I13504
  • J23106 + I13504
  • J23117 + I13504

On LB + Ampicillin 100ug/mL Incubation ON, 37°C


by Johan and Seong Ko

R0051 On LB + Ampicillin 100ug/mL Incubation ON, 37°C

Plasmid Rehydratation :

by Johan and Audrey

Biobricks:

  • S03518
  • B0030
  • B0015
  • K1399005

Digestion

by Pauline and Audrey

Plasmid with promotor: J23101

  • 10µL of our plasmid with promotor
  • 1µL SpeI
  • 1µL PstI
  • 2µL buffer 10x FD

Plasmid with gene: COO40 and K115017

  • 10µL of our plasmid with gene
  • 1µL XbaI
  • 1µL PstI
  • 2µL buffer 10x FD

After this step, we separate the sequence we need from the sequence we don't with electrophoresis

Electrophoresis

by Pauline and Audrey

Preparation of Agarose Gel 1%, 0,5g in 50mL of 1X TAE, 0,5µL of BET Migration 0,06A 80V

Quantification

by Pauline and Audrey

Preparation of Agarose Gel 1%, 0,5g in 50mL of 1X TAE, 0,5µL of BET Migration 0,06A 80V We quantify with the electrophoresis gel:

  • C0040: 10ng/µL
  • J23101: 20ng/µL
  • K115017: we don't see enough of fluorescent to quantify the biobrick. We decide to amplify this one by PCR.

Members present:

  • Instructors and advisors: Alice.
  • Students: Johan, Seong Koo, Audrey, Coralie, Pauline