Difference between revisions of "Team:IONIS Paris/Description"

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<h2> Bio-Game </h2>
 
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<img src="https://static.igem.org/mediawiki/2015/c/c2/Schema1_IONIS.png"  width="30%" height="70%" style="float:right;" >
 
The main goal of <FONT COLOR=#800000><b>IONIS iGEM</b></FONT> team is to make synthetic biology more accessible to people. Indeed, this field is not well understood especially in France and as every big innovation or scientific tool people are afraid and against it when it comes to <b>public knowledge</b>.</br></br>
 
Our team decided to break all the prejudices regarding synthetic biology by presenting it as an efficient scientific tool that can be controlled and that can be without any risk if well engineered.
 
</br>
 
Our project is based on <FONT COLOR=#006666><b>the popularization of synthetic biology</b></FONT> using two approaches:</br>
 
<ul>
 
<li>
 
          The first one is the design of a real synthetic biology game: our <b>“Bio-console”</b>.
 
It is based on the control of engineered bacteria in a micro-fluidic chip connected to a computer interface.
 
Our game is dependent on <b>optogenetic</b> signals leading to either the fluorescence of our bacteria in order to visualize it or the death of our bacteria in order to control our system leading to an efficient kill switch.
 
</li></br>
 
<li>
 
The second one is the design of a <b>mobile application</b> game presenting several concepts of Biology, Synthetic Biology & safety.
 
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Our team wants to promote Synthetic Biology using an original point of view. The goal is to make this field accessible to everyone by using a playful & entertaining tool.
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<b>Key words:</b>    Synthetic Biology, Optogenetics, BioSafety, Kill Switch, Scientific vulgarization, Light, Game, Engineering, Micro fluidic, Bacteria, BACT'MAN, Bio-Console
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<h1 class="intro wow zoomIn" wow-data-delay="0.4s" wow-data-duration="0.9s"  style="font-family:sans-serif; color:#B00000 " >Our project</h1>
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<h2 class="text-center wowload fadeInUp" style="color:#ff0000">Popularization of Synthetic Biology</h2>
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<h3 class="text-center wowload fadeInUp">By 2 different approaches</h3> 
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<h4><i class="fa fa-gamepad" style="color:#b22222"></i> Design of a real synthetic biology game: the Bio-console </h4>
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<p align="justify">It is based on the control of engineered bacteria in a microfluidic chip connected to a computer interface. Our game is dependent on <font style="color:#66cdaa">optogenetic signals</font> leading to either the fluorescence of our bacteria in order to visualize it, or the death of our bacteria. The latter allows us to control our system leading to an efficient kill switch.</p>
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<h4><i class="fa fa-mobile" style="color:#b22222"></i> The mobile application: Biology, Synthetic Biology & Safety! </h4>
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<p align="justify"> Our team wants to<font style="color:#66cdaa"> promote Synthetic Biology</font> using an original point of view. The goal is to make this field accessible to everyone by using a playful & entertaining tool..</p>
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<h2 class="text-center  wowload fadeInUp">What do we do exactly?</h2>
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<p style="font-style:italic;color:red;border-style:solid;border-width:2px;border-color:red">Your browser either does not support HTML5 or cannot handle MediaWiki open video formats. Please consider upgrading your browser, installing the appropriate plugin or switching to a Firefox or Chrome install.</p>
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<a class="myButton" href="https://www.youtube.com/watch?v=gVtXKnW4zD0" style="color:#b0000f;background-color: #FFFFFF;">English Subtitles</a>
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<h2 class="text-center wowload fadeInUp" style="color:#ff0000">The Bio-Console </h2>
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<p align="left" ><img src="https://static.igem.org/mediawiki/2015/8/88/Schema_IONIS.png" alt="Project"  width="500" height=auto ></p>
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<h4><i class="fa fa-gamepad" style="color:#b22222"></i> How will work our Bio-Console ? </h4>
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<p align="justify">To begin, we need our bacteria to be visible to the player. That's why we added the genes responsible for bacteria fluorescence.
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Our genetic construction will allow us to <font style="color:#66cdaa">induce bacteria fluorescence</font> through light signals.
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In this way, we can make each group of bacteria directly visible before starting of the game. 
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Then, the bacteria will be introduced in an aqueous <font style="color:#66cdaa">medium </font> having the same density as the water.
 +
In this environment, the bacteria will have all the molecules that allow them to survive in the game.
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There will be as well some liposomes (small capsules where the internal environment is separated by membranes from the external environment).
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Inside of these liposomes, bacteria destructive toxins will be present, and this is where the <font style="color:#66cdaa">gameplay</font> of our game relies. 
 +
Once the bacteria are fluorescent, we will introduce the aqueous medium in our microfluidic chip.
 +
This chip will be placed beforehand under an optical microscope itself connected to the computer screen.
 +
The aqueous medium, then forms a sort of bubble containing <font style="color:#66cdaa">BACT'Man</font> and toxins trapped in the vesicle.
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This bubble will be directly controlled by the player by flows through the joystick as it is related to the computer.
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</p>
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<h4><i class="fa fa-mobile" style="color:#b22222"></i> The goal of the game !</h4>
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<p align="justify"> The goal is simple: to allow <font style="color:#66cdaa">BACT'Man</font> to survive as long as possible.
 +
Stop him from getting in contact with the virtual lasers that will appear faster and faster as the game goes on.
 +
If <font style="color:#66cdaa">BACT'Man</font> were to be touched, a bright flash will appear at the microscope and will activate the liposomes opening.
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The toxins will eliminate our hero that will lose his fluorescence ... <font style="color:#66cdaa"><b>GAME OVER</b></font>
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<h2>BIOBRICKS</h2>
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<p>Our parts of DNA<br>
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<a href="https://2015.igem.org/Team:IONIS_Paris/Biobricks" >View more</a></p>           
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<h2>OPTOGENETICS</h2>
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<p>Our scientific core of the project<br>
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<a href="https://2015.igem.org/Team:IONIS_Paris/Project/Optogenetics" >View more</a></p>           
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<h2>MICROFLUIDICS</h2>
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<p>Our challenge in achieving the Bio-console<br>
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<a href="https://2015.igem.org/Team:IONIS_Paris/Microfluidics" >View more</a></p>           
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<h3 class="title">Title</h3>
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Revision as of 11:48, 27 August 2015


IONIS Paris

Popularization of Synthetic Biology

By 2 different approaches

Design of a real synthetic biology game: the Bio-console

It is based on the control of engineered bacteria in a microfluidic chip connected to a computer interface. Our game is dependent on optogenetic signals leading to either the fluorescence of our bacteria in order to visualize it, or the death of our bacteria. The latter allows us to control our system leading to an efficient kill switch.

The mobile application: Biology, Synthetic Biology & Safety!

Our team wants to promote Synthetic Biology using an original point of view. The goal is to make this field accessible to everyone by using a playful & entertaining tool..

Mobile Application



What do we do exactly?



English Subtitles

The Bio-Console

Project

How will work our Bio-Console ?

To begin, we need our bacteria to be visible to the player. That's why we added the genes responsible for bacteria fluorescence. Our genetic construction will allow us to induce bacteria fluorescence through light signals. In this way, we can make each group of bacteria directly visible before starting of the game. Then, the bacteria will be introduced in an aqueous medium having the same density as the water. In this environment, the bacteria will have all the molecules that allow them to survive in the game. There will be as well some liposomes (small capsules where the internal environment is separated by membranes from the external environment). Inside of these liposomes, bacteria destructive toxins will be present, and this is where the gameplay of our game relies. Once the bacteria are fluorescent, we will introduce the aqueous medium in our microfluidic chip. This chip will be placed beforehand under an optical microscope itself connected to the computer screen. The aqueous medium, then forms a sort of bubble containing BACT'Man and toxins trapped in the vesicle. This bubble will be directly controlled by the player by flows through the joystick as it is related to the computer.

The goal of the game !

The goal is simple: to allow BACT'Man to survive as long as possible. Stop him from getting in contact with the virtual lasers that will appear faster and faster as the game goes on. If BACT'Man were to be touched, a bright flash will appear at the microscope and will activate the liposomes opening. The toxins will eliminate our hero that will lose his fluorescence ... GAME OVER

team

BIOBRICKS

Our parts of DNA
View more

team

OPTOGENETICS

Our scientific core of the project
View more

team

MICROFLUIDICS

Our challenge in achieving the Bio-console
View more