Revision as of 19:43, 21 July 2015

Tuesday 7th July

by Coralie, Johan, Seong Koo, Audrey, Pauline

With the Nucleospin Kit from Macherey Nagel

Stock solution preparation :

We use 18µL from this solution with 2µL plasmid solution

Incubation 1h30 at 37°C

by Johan, Audrey, Pauline

Preparation of Agarose Gel 1%, 0,5g in 50mL of 1X TAE, 0,5uL of BET Migration 0,06A 80V

by Pauline, Audrey

New liquid culture of:

2x 5ml LB + 10μl Chloramphenicol + 1 bacterial colony. We incubate cultures at 37°C, ON.

Members present:

@@ Line 1: / Line 1: @@
+{{Team:Paris_Saclay/notebook_header}}
 =Tuesday 7th July=
-==Back in Lab==
+==Labwork==
-====Verification of our plasmids====
+===Verification of our plasmids===
 ''by Coralie, Johan, Seong Koo, Audrey, Pauline''
-=====Biobricks verification:=====
+====Biobricks verification====
 *K115017
 *C0040
@@ Line 14: / Line 14: @@
 *I13504
-=====Extraction=====
+====Extraction====
 With the Nucleospin Kit from Macherey Nagel
-=====Digestion by NotI: =====
+====Digestion by NotI====
 Stock solution preparation :
 * 124µL H2O
@@ Line 29: / Line 27: @@
 Incubation 1h30 at 37°C
-=====Electrophoresis=====
+====Electrophoresis====
 ''by Johan, Audrey, Pauline''
@@ Line 35: / Line 33: @@
 Migration 0,06A 80V
+===New culture===
-====New culture====
 ''by Pauline, Audrey''
@@ Line 44: / Line 41: @@
 x 5ml LB + 10μl Chloramphenicol + 1 bacterial colony.
 We incubate cultures at 37°C, ON.
 '''Members present:'''
 * Instructors and advisors: Alice.
 * Students: Johan, Seong Koo, Audrey, Coralie, Pauline
+{{Team:Paris_Saclay/notebook_footer}}