Difference between revisions of "Team:Paris Saclay/Notebook/July/17"
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| + | {{Team:Paris_Saclay/notebook_header}} | ||
=Friday 17th July= | =Friday 17th July= | ||
| − | + | ==Lab Work== | |
| − | + | ===Ligation=== | |
| − | ==== | + | |
''by Pauline'' | ''by Pauline'' | ||
| Line 10: | Line 10: | ||
* 4µL H2O | * 4µL H2O | ||
| − | + | * BBa_J23101 (10µL) + BBa_S03518 (12µL) | |
| − | * | + | * BBa_S03518 (12µL) + BBa_B0015 (10µL) |
| − | * | + | * BBa_B0015 (10µL) + BBa_I13602 (12µL) |
| − | * | + | |
| − | Incubation 4h, 4°C | + | Incubation 4h, 4°C |
| − | + | ===New culture=== | |
| − | + | ||
| − | + | ||
''by Coralie'' | ''by Coralie'' | ||
| Line 28: | Line 25: | ||
On LB plates, incubation ON, 37°C | On LB plates, incubation ON, 37°C | ||
| − | + | ===Transformation=== | |
| − | + | ||
''by Johan'' | ''by Johan'' | ||
Transformation with ligation product: | Transformation with ligation product: | ||
| − | * | + | * BBa_J23101 + BBa_S03518 = BBa_K1707001 |
| − | * | + | * BBa_B0015 + BBa_I13602 = BBa_K1707002 |
| − | * | + | * BBa_S03518 + BBa_B0015 = BBa_K1707003 |
| − | + | ===New culture on plates=== | |
| − | + | ||
''by Pauline'' | ''by Pauline'' | ||
| − | * | + | * BBa_J23101 + BBa_I13504 |
| − | * | + | * BBa_J23106 + BBa_I13504 |
| − | * | + | * BBa_J23117 + BBa_I13504 |
| − | * | + | * BBa_B0030 |
| − | * | + | * BBa_R0051 |
| − | + | ||
| − | + | ===Plasmid extraction=== | |
''by Pauline and Seong-Koo'' | ''by Pauline and Seong-Koo'' | ||
| − | + | * BBa_K1707000 | |
| − | * | + | * BBa_K1399019 |
| − | * | + | * BBa_K1399005 |
| − | * | + | * BBa_K1399023 |
| − | * | + | |
Cultures from the 16/15/2015 | Cultures from the 16/15/2015 | ||
With the Nucleospin Kit from Macherey Nagel | With the Nucleospin Kit from Macherey Nagel | ||
| − | |||
| − | |||
| − | |||
| − | |||
'''Members present:''' | '''Members present:''' | ||
* Instructors and advisors: Alice. | * Instructors and advisors: Alice. | ||
* Students: Johan, Seong Koo, Coralie, Pauline | * Students: Johan, Seong Koo, Coralie, Pauline | ||
| + | {{Team:Paris_Saclay/notebook_header}} | ||
Revision as of 20:05, 21 July 2015
Contents
Friday 17th July
Lab Work
Ligation
by Pauline
In each tube:
- 3 µL Buffer DNA pol T4
- 1µL DNA pol T4
- 4µL H2O
- BBa_J23101 (10µL) + BBa_S03518 (12µL)
- BBa_S03518 (12µL) + BBa_B0015 (10µL)
- BBa_B0015 (10µL) + BBa_I13602 (12µL)
Incubation 4h, 4°C
New culture
by Coralie
- 1696: Bad::Tet
- 1320:ClpP::Cm
- 1693:z1:Spect
On LB plates, incubation ON, 37°C
Transformation
by Johan
Transformation with ligation product:
- BBa_J23101 + BBa_S03518 = BBa_K1707001
- BBa_B0015 + BBa_I13602 = BBa_K1707002
- BBa_S03518 + BBa_B0015 = BBa_K1707003
New culture on plates
by Pauline
- BBa_J23101 + BBa_I13504
- BBa_J23106 + BBa_I13504
- BBa_J23117 + BBa_I13504
- BBa_B0030
- BBa_R0051
Plasmid extraction
by Pauline and Seong-Koo
- BBa_K1707000
- BBa_K1399019
- BBa_K1399005
- BBa_K1399023
Cultures from the 16/15/2015 With the Nucleospin Kit from Macherey Nagel
Members present:
- Instructors and advisors: Alice.
- Students: Johan, Seong Koo, Coralie, Pauline