Difference between revisions of "Team:EPF Lausanne/Notebook/Protocols"
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| − | <div class="first-section | + | <div class="first-section"> |
| − | + | <div class="row"> | |
| − | + | <nav class="col-sm-3" id="myScrollspy"> | |
| − | + | <ul class="nav nav-pills nav-stacked"> | |
| − | < | + | <li class="active"><a href="#section1">Section 1</a></li> |
| − | + | <li><a href="#section2">Section 2</a></li> | |
| − | + | <li><a href="#section3">Section 3</a></li> | |
| − | + | <li class="dropdown"> | |
| − | + | <a class="dropdown-toggle" data-toggle="dropdown" href="#">Section 4 <span class="caret"></span></a> | |
| − | + | <ul class="dropdown-menu"> | |
| − | + | <li><a href="#section41">Section 4-1</a></li> | |
| − | </li> | + | <li><a href="#section42">Section 4-2</a></li> |
| − | <li> <a href="# | + | </ul> |
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| − | + | <div class="col-sm-9"> | |
| − | + | <div id="section1" class="well"> | |
| − | + | <h1>Agarose Gel Preparation</h1> | |
| − | + | <h2>1,2% agarose gel – for small DNA fragments</h2> | |
| − | + | <p>Mix 50 mL 1X TAE and 0.6 g Agarose</p> | |
| − | + | <p>Melt in microwave until agarose has melted (about 50 seconds)</p> | |
| + | <p>Add 1.3 μL Gel Red</p> | ||
| + | <p>Pour solution into agarose gel mold with comb</p> | ||
| + | <p>Let set for 20 minutes or until solid</p> | ||
| + | <p>Place gel in 1X TAE and remove comb</p> | ||
| + | <p>Load samples of 200 ng (or 2 μL) DNA mixed with 2 μL 6X loading dye and water up to 12 μL</p> | ||
| + | <p>Run gel at 100-120 Volts for 40-50 minutes (depending on size of fragments)</p> | ||
| + | <p>Take a picture of the gel at the UV detector</p> | ||
| + | <h2>3% agarose gel – for large DNA fragments</h2> | ||
| + | <p>Mix 50 mL 1X TAE and 1.5 g Agarose</p> | ||
| + | <p>Melt in microwave until agarose has melted (about 50 seconds)</p> | ||
| + | <p>Add 1.5 μL Gel Red</p> | ||
| + | <p>Pour solution into agarose gel mold with comb</p> | ||
| + | <p>Let set for 20 minutes or until solid</p> | ||
| + | <p>Place gel in 1X TAE and remove comb</p> | ||
| + | <p>Load samples of 200 ng (or 2 μL) DNA mixed with 2 μL 6X loading dye and water up to 12 μL</p> | ||
| + | <p>Run gel at 80 Volts for 2 hours</p> | ||
| + | <p>Take a picture of the gel at the UV detector</p> | ||
| + | </div> | ||
| + | <div id="section2" class="well"> | ||
| + | <h1>Section 2</h1> | ||
| + | <p>Try to scroll this section and look at the navigation list while scrolling!</p> | ||
| + | </div> | ||
| + | <div id="section3" class="well"> | ||
| + | <h1>Section 3</h1> | ||
| + | <p>Try to scroll this section and look at the navigation list while scrolling!</p> | ||
| + | </div> | ||
| + | <div id="section41" class="well"> | ||
| + | <h1>Section 4-1</h1> | ||
| + | <p>Try to scroll this section and look at the navigation list while scrolling!</p> | ||
| + | </div> | ||
| + | <div id="section42" class="well"> | ||
| + | <h1>Section 4-2</h1> | ||
| + | <p>Try to scroll this section and look at the navigation list while scrolling!</p> | ||
| + | </div> | ||
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Revision as of 12:21, 21 July 2015
Protocols
Agarose Gel Preparation
1,2% agarose gel – for small DNA fragments
Mix 50 mL 1X TAE and 0.6 g Agarose
Melt in microwave until agarose has melted (about 50 seconds)
Add 1.3 μL Gel Red
Pour solution into agarose gel mold with comb
Let set for 20 minutes or until solid
Place gel in 1X TAE and remove comb
Load samples of 200 ng (or 2 μL) DNA mixed with 2 μL 6X loading dye and water up to 12 μL
Run gel at 100-120 Volts for 40-50 minutes (depending on size of fragments)
Take a picture of the gel at the UV detector
3% agarose gel – for large DNA fragments
Mix 50 mL 1X TAE and 1.5 g Agarose
Melt in microwave until agarose has melted (about 50 seconds)
Add 1.5 μL Gel Red
Pour solution into agarose gel mold with comb
Let set for 20 minutes or until solid
Place gel in 1X TAE and remove comb
Load samples of 200 ng (or 2 μL) DNA mixed with 2 μL 6X loading dye and water up to 12 μL
Run gel at 80 Volts for 2 hours
Take a picture of the gel at the UV detector
Section 2
Try to scroll this section and look at the navigation list while scrolling!
Section 3
Try to scroll this section and look at the navigation list while scrolling!
Section 4-1
Try to scroll this section and look at the navigation list while scrolling!
Section 4-2
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