Difference between revisions of "Team:EPF Lausanne/Notebook/Protocols"
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| − | + | <ul class="nav nav-pills nav-stacked"> | |
| − | + | <li class="active"><a href="#agarosegel">Agarose Gel Preparation</a></li> | |
| − | + | <li><a href="#section2">Section 2</a></li> | |
| − | + | <li><a href="#section3">Section 3</a></li> | |
| − | + | <li class="dropdown"> | |
| − | + | <a class="dropdown-toggle" data-toggle="dropdown" href="#">Section 4 <span class="caret"></span></a> | |
| − | + | <ul class="dropdown-menu"> | |
| − | + | <li><a href="#section41">Section 4-1</a></li> | |
| − | + | <li><a href="#section42">Section 4-2</a></li> | |
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| − | + | <div id="agarosegel" class="well"> | |
| − | <div class="col- | + | <h1>Agarose Gel Preparation</h1> |
| − | + | <h2>Materials</h2> | |
| − | + | <p> • 1X TAE</p> | |
| − | + | <p> • Agarose</p> | |
| − | + | <p> • Gel Red</p> | |
| − | + | <p> • DNA samples</p> | |
| − | + | <p> • 6X loading dye</p> | |
| − | + | <p> • Nuclease free water</p> | |
| − | + | <h2>Procedure</h2> | |
| − | + | <p>Prepare 1.2% agarose gel for small fragments and 3% agarose gel for large fragments</p> | |
| − | + | <p> • Mix 50 mL 1X TAE and 0.6 g (1.2%) or 1.5 g (3%) agarose</p> | |
| − | + | <p> • Melt in microwave until agarose has melted (about 50 seconds)</p> | |
| − | + | <p> • Add 1.3 μL (1.2%) or 1.5 μL (3%) Gel Red</p> | |
| − | + | <p> • Pour solution into agarose gel mold with comb</p> | |
| − | + | <p> • Let set for 20 minutes or until solid</p> | |
| − | + | <p> • Place gel in 1X TAE and remove comb</p> | |
| − | + | <p> • Load samples of 200 ng (or 2 μL) DNA mixed with 2 μL 6X loading dye and nuclease free water up to 12 μL</p> | |
| − | + | <p> • Run gel at 100-120 Volts for 40-50 minutes (1.2%) or 80 Volts for 2 hours (3%)</p> | |
| − | + | <p> • Take a picture of the gel at the UV detector</p> | |
| − | + | </div> | |
| − | + | <div id="section2" class="well"> | |
| − | + | <h1>Section 2</h1> | |
| − | + | <p>Try to scroll this section and look at the navigation list while scrolling!</p> | |
| − | + | </div> | |
| − | + | <div id="section3" class="well"> | |
| − | + | <h1>Section 3</h1> | |
| − | + | <p>Try to scroll this section and look at the navigation list while scrolling!</p> | |
| − | + | </div> | |
| − | + | <div id="section41" class="well"> | |
| − | + | <h1>Section 4-1</h1> | |
| − | + | <p>Try to scroll this section and look at the navigation list while scrolling!</p> | |
| − | + | </div> | |
| − | + | <div id="section42" class="well"> | |
| − | + | <h1>Section 4-2</h1> | |
| − | + | <p>Try to scroll this section and look at the navigation list while scrolling!</p> | |
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Revision as of 12:34, 21 July 2015
Protocols
Agarose Gel Preparation
Materials
• 1X TAE
• Agarose
• Gel Red
• DNA samples
• 6X loading dye
• Nuclease free water
Procedure
Prepare 1.2% agarose gel for small fragments and 3% agarose gel for large fragments
• Mix 50 mL 1X TAE and 0.6 g (1.2%) or 1.5 g (3%) agarose
• Melt in microwave until agarose has melted (about 50 seconds)
• Add 1.3 μL (1.2%) or 1.5 μL (3%) Gel Red
• Pour solution into agarose gel mold with comb
• Let set for 20 minutes or until solid
• Place gel in 1X TAE and remove comb
• Load samples of 200 ng (or 2 μL) DNA mixed with 2 μL 6X loading dye and nuclease free water up to 12 μL
• Run gel at 100-120 Volts for 40-50 minutes (1.2%) or 80 Volts for 2 hours (3%)
• Take a picture of the gel at the UV detector
Section 2
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Section 3
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Section 4-1
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Section 4-2
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