Difference between revisions of "Team:EPF Lausanne/Timeline"
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<p>The E. Coli part of our project is strongly based on the papar of Bikard et al. "Programmable repression and activation of bacterial gene expression using an engineered CRISPR-Cas system". Bikard sent us his strains JEN202, JEN202+PWJ89 and DH5A+PWJ66. We also ordered a plasmid containing dCas9 and the synthetic DNA encoding fou our gRNAs.</p> | <p>The E. Coli part of our project is strongly based on the papar of Bikard et al. "Programmable repression and activation of bacterial gene expression using an engineered CRISPR-Cas system". Bikard sent us his strains JEN202, JEN202+PWJ89 and DH5A+PWJ66. We also ordered a plasmid containing dCas9 and the synthetic DNA encoding fou our gRNAs.</p> | ||
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<p>...the new framework for biologic circuits using dCas9.</p> | <p>...the new framework for biologic circuits using dCas9.</p> | ||
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Revision as of 16:50, 28 July 2015
Timeline
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Mussum ipsum cacilds
Mussum ipsum cacilds, vidis litro abertis. Consetis adipiscings elitis. Pra lá , depois divoltis porris, paradis. Paisis, filhis, espiritis santis. Mé faiz elementum girarzis, nisi eros vermeio, in elementis mé pra quem é amistosis quis leo. Manduma pindureta quium dia nois paga. Sapien in monti palavris qui num significa nadis i pareci latim. Interessantiss quisso pudia ce receita de bolis, mais bolis eu num gostis.
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Mussum ipsum cacilds
Mussum ipsum cacilds, vidis litro abertis. Consetis adipiscings elitis. Pra lá , depois divoltis porris, paradis. Paisis, filhis, espiritis santis. Mé faiz elementum girarzis, nisi eros vermeio, in elementis mé pra quem é amistosis quis leo. Manduma pindureta quium dia nois paga. Sapien in monti palavris qui num significa nadis i pareci latim. Interessantiss quisso pudia ce receita de bolis, mais bolis eu num gostis.
Suco de cevadiss, é um leite divinis, qui tem lupuliz, matis, aguis e fermentis. Interagi no mé, cursus quis, vehicula ac nisi. Aenean vel dui dui. Nullam leo erat, aliquet quis tempus a, posuere ut mi. Ut scelerisque neque et turpis posuere pulvinar pellentesque nibh ullamcorper. Pharetra in mattis molestie, volutpat elementum justo. Aenean ut ante turpis. Pellentesque laoreet mé vel lectus scelerisque interdum cursus velit auctor. Lorem ipsum dolor sit amet, consectetur adipiscing elit. Etiam ac mauris lectus, non scelerisque augue. Aenean justo massa.
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Mussum ipsum cacilds
Mussum ipsum cacilds, vidis litro abertis. Consetis adipiscings elitis. Pra lá , depois divoltis porris, paradis. Paisis, filhis, espiritis santis. Mé faiz elementum girarzis, nisi eros vermeio, in elementis mé pra quem é amistosis quis leo. Manduma pindureta quium dia nois paga. Sapien in monti palavris qui num significa nadis i pareci latim. Interessantiss quisso pudia ce receita de bolis, mais bolis eu num gostis.
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Mussum ipsum cacilds
Mussum ipsum cacilds, vidis litro abertis. Consetis adipiscings elitis. Pra lá , depois divoltis porris, paradis. Paisis, filhis, espiritis santis. Mé faiz elementum girarzis, nisi eros vermeio, in elementis mé pra quem é amistosis quis leo. Manduma pindureta quium dia nois paga. Sapien in monti palavris qui num significa nadis i pareci latim. Interessantiss quisso pudia ce receita de bolis, mais bolis eu num gostis.
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Mussum ipsum cacilds
Mussum ipsum cacilds, vidis litro abertis. Consetis adipiscings elitis. Pra lá , depois divoltis porris, paradis. Paisis, filhis, espiritis santis. Mé faiz elementum girarzis, nisi eros vermeio, in elementis mé pra quem é amistosis quis leo. Manduma pindureta quium dia nois paga. Sapien in monti palavris qui num significa nadis i pareci latim. Interessantiss quisso pudia ce receita de bolis, mais bolis eu num gostis.
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The bible
The E. Coli part of our project is strongly based on the papar of Bikard et al. "Programmable repression and activation of bacterial gene expression using an engineered CRISPR-Cas system". Bikard sent us his strains JEN202, JEN202+PWJ89 and DH5A+PWJ66. We also ordered a plasmid containing dCas9 and the synthetic DNA encoding fou our gRNAs.
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Blast
02.04.2015
In our project dCas9 target DNA sequences using gRNAs and acts as an activator or an inhibitor depending on its position. Axel and Cyril created a C++ program to blast random generated gRNA sequences on the E. Coli or Yeast genome.
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And the winner is...
26.03.2015
...the new framework for biologic circuits using dCas9.
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The lab starts!
24.03.2015 - 26.03.2015
TAs start to train us in the lab: medium and agar plates preparation
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Brainstorming finalists
19.03.2015
Three cool ideas came out of the brainstorming: new framework for biologic circuits or lung cancer therapy using dCas9 and cell-cell communication.
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Brainstorming
26.02.2015 - 12.03.2015
The team is split in small groups, brainstorming for an interesting project that will occupy us during the summer.
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First meeting: iGEM officially starts!
19.02.2015
First meeting with everyone: 12 teammates, 7 TAs and 3 advisors.