Difference between revisions of "Team:Paris Saclay/Notebook/July/27"
(→Friday 27th July) |
|||
| Line 12: | Line 12: | ||
''by Pauline and Coralie'' | ''by Pauline and Coralie'' | ||
| − | + | * BBa_K1707008 | |
| − | * 2µL Tp 10x | + | ** 2µL Tp 10x |
| − | * 1µL Ligase | + | ** 1µL Ligase |
| − | * 11µL BBa_K1707003 | + | ** 11µL BBa_K1707003 |
| − | * 6µL BBa_J23101 | + | ** 6µL BBa_J23101 |
| − | + | * BBa_K1707010 | |
| − | * 1µL Tp 10x | + | ** 1µL Tp 10x |
| − | * 1µL Ligase | + | ** 1µL Ligase |
| − | * 12µL BBa_K1399005 | + | ** 12µL BBa_K1399005 |
| − | * 10µL BBa_B0015 | + | ** 10µL BBa_B0015 |
| − | * 1µL H20 | + | ** 1µL H20 |
| − | + | ||
| − | * 2µL Tp 10x | + | * BBa_K1707009 |
| − | * 2µL Ligase | + | ** 2µL Tp 10x |
| − | * 10µL BBa_S03518 | + | ** 2µL Ligase |
| − | * 6µL BBa_R0051 | + | ** 10µL BBa_S03518 |
| + | ** 6µL BBa_R0051 | ||
| + | |||
| + | * BBa_K1707004 | ||
| + | ** 3µL Tp 10x | ||
| + | ** 2µL Ligase | ||
| + | ** 8µL BBa_K1707003 | ||
| + | ** 7µL BBa_R0051 | ||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
===Transformation=== | ===Transformation=== | ||
Revision as of 08:27, 5 August 2015
Contents
Friday 27th July
Lab Work
New culture
by Seong Koo
Biobricks:
- BBa_K1707005
- BBa_K1707006
- BBa_K1707007
Ligation
by Pauline and Coralie
- BBa_K1707008
- 2µL Tp 10x
- 1µL Ligase
- 11µL BBa_K1707003
- 6µL BBa_J23101
- BBa_K1707010
- 1µL Tp 10x
- 1µL Ligase
- 12µL BBa_K1399005
- 10µL BBa_B0015
- 1µL H20
- BBa_K1707009
- 2µL Tp 10x
- 2µL Ligase
- 10µL BBa_S03518
- 6µL BBa_R0051
- BBa_K1707004
- 3µL Tp 10x
- 2µL Ligase
- 8µL BBa_K1707003
- 7µL BBa_R0051
Transformation
by Pauline and Coralie
Biobricks:
- BBa_K1707008
- BBa_K1707010
- BBa_K1707009
- BBa_K1707004
Quantification
Biobricks:
- BBa_K1707003
- BBa_K1399005
- BBa_B0015
- BBa_J23101
Agarose gel 1% Migration 110V
Transformation
by Seong-Koo
Biobricks:
- BBa_K1707005
- BBa_K1707006
- BBa_K1707007
Soil experiment
by Seong Koo
Plates observation and count of CFU:
- Strain 1696:
- Dilution 1: 424 CFU
- Dilution 0.1: 75 CFU
- Dilution 0.01: 1 CFU
- Strain 1693:
- Dilution 1: 524 CFU
- Dilution 0.1: 35 CFU
- Dilution 0.01: 0 CFU
- Strain 1320:
- Dilution 1: 111 CFU
- Dilution 0.1: 13 CFU
- Dilution 0.01: 0 CFU
We take 1g of contaminated soil and treat it like yesterday to spread it on specific plates Incubation ON, 37°C
Member present:
- Instructors: Alice
- Students: Coralie, Audrey, Pauline, Johan and Seong-Koo
Contact
eigem.parissaclay AT gmail DOT com
t@iGEMParisSaclay
aUniversité Paris Sud
a91405 Orsay, France
Copyright © 2015 iGEM Paris Saclay. All rights reserved All inspirations encouraged.
*This wiki was conceived during long nights of work and a great coffee experience in the beautiful Orsay, France.
eigem.parissaclay AT gmail DOT com
t@iGEMParisSaclay
aUniversité Paris Sud
a91405 Orsay, France
Copyright © 2015 iGEM Paris Saclay. *This wiki was conceived during long nights of work and a great coffee experience in the beautiful Orsay, France.