Difference between revisions of "Safety/Risk Groups"
(re-added the "Common iGEM organisms" list, because i lost it somehow last time) |
(more small text tweaks / moved "animals and plants" up to top section) |
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<br /> | <br /> | ||
<table> | <table> | ||
+ | <tr> | ||
+ | <th> </th> | ||
+ | <th colspan="2">Permitted in iGEM</th> | ||
+ | <th colspan="2">Not permitted in iGEM</th> | ||
+ | </tr> | ||
<tr> | <tr> | ||
<th> </th> | <th> </th> | ||
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<tr> | <tr> | ||
<th>Examples</th> | <th>Examples</th> | ||
− | <td><i>E. coli</i> K-12, | + | <td><i>E. coli</i> K-12, <i>S. cerevisiae</i> (yeast), <i>Lactobacillus</i>, <i>B. subtilis</i></td> |
<td><i>Streptococcus</i>, Herpes virus, most mammalian cell lines</td> | <td><i>Streptococcus</i>, Herpes virus, most mammalian cell lines</td> | ||
− | <td><i>Yersinia pestis</i> (black plague), HIV, SARS virus</td> | + | <td><i>Yersinia pestis</i> (black plague), HIV, SARS virus</td> |
<td>Ebola virus, Marburg virus, Lassa virus</td> | <td>Ebola virus, Marburg virus, Lassa virus</td> | ||
</tr> | </tr> | ||
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<th>Typical Work Area</th> | <th>Typical Work Area</th> | ||
<td>Open bench</td> | <td>Open bench</td> | ||
− | <td>Biosafety cabinet / laminar flow hood</td> | + | <td>Biosafety cabinet / laminar flow hood</td> |
<td>Class 3 biosafety cabinet</td> | <td>Class 3 biosafety cabinet</td> | ||
<td>Full isolation suits</td> | <td>Full isolation suits</td> | ||
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</table> | </table> | ||
+ | <br /> | ||
+ | <h4>Animals and plants have no Risk Group</h4> | ||
+ | |||
+ | <p>The four Risk Groups only apply to microorganisms (bacteria, fungi, and viruses). For animals, plants, and other multicellular organisms, there are different safety guidelines. Please consult with your advisor or university authorities if you plan to do experiments with animals and plants.</p> | ||
+ | |||
+ | <p>If you are using a part from an animal or plant, you should consider the <strong>function</strong> of the part, and consider whether it might be dangerous to humans. For example, the gene for Green Fluorescent Protein (GFP) comes from the jellyfish <i>Aequorea victoria</i>, which does not have a Risk Group. GFP is only a fluorescent protein, absorbing and emitting light, so it is safe for humans and you can use it in a Safety Level 1 lab. But if you consider a different gene from <i>A. victoria</i>, such as the gene for the toxin in its stinging tentacles, you might conclude that you should only use that gene under strict safety conditions!</p> | ||
<br /> | <br /> | ||
<br /> | <br /> | ||
− | <h2><a class="anchor" id=" | + | <h2><a class="anchor" id="HowToFindRiskGroup"></a>How can I find out the Risk Group of my organism?</h2> |
<h4>1. Common iGEM Organisms</h4> | <h4>1. Common iGEM Organisms</h4> | ||
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</div> | </div> | ||
− | <p>DSMZ (Deutsche Sammlung von Mikroorganismen und Zellkulturen / German Collection of Microorganisms and Cell Cultures) is a large collection that includes Risk Group information for thousands of different species, strains, and cell lines. It is more difficult to use, but it includes the most species of any source | + | <p>DSMZ (Deutsche Sammlung von Mikroorganismen und Zellkulturen / German Collection of Microorganisms and Cell Cultures) is a large collection that includes Risk Group information for thousands of different species, strains, and cell lines. It is more difficult to use, but it includes the most species of any source that iGEM recommends.</p> |
<ul> | <ul> | ||
<li><a href="http://www.dsmz.de/catalogues/catalogue-microorganisms.html">Catalogue Microorganisms</a> -- search bacteria. Enter your genus, species, and/or strain information in the "Search term" box, and click Search. You will receive a long list of results, which may include many different strains and species. Examine the list to find the correct strain, and click on its DSM number to view its catalogue page. The Risk Group is listed in the table.</li> | <li><a href="http://www.dsmz.de/catalogues/catalogue-microorganisms.html">Catalogue Microorganisms</a> -- search bacteria. Enter your genus, species, and/or strain information in the "Search term" box, and click Search. You will receive a long list of results, which may include many different strains and species. Examine the list to find the correct strain, and click on its DSM number to view its catalogue page. The Risk Group is listed in the table.</li> | ||
<li><a href="http://www.dsmz.de/catalogues/catalogue-human-and-animal-cell-lines.html">Catalogue Human and Animal Cell Lines</a> -- search this catalogue by the same method as for Catalogue Microorganisms.</li> | <li><a href="http://www.dsmz.de/catalogues/catalogue-human-and-animal-cell-lines.html">Catalogue Human and Animal Cell Lines</a> -- search this catalogue by the same method as for Catalogue Microorganisms.</li> | ||
− | <li><a href="http://www.dsmz.de/bacterial-diversity/prokaryotic-nomenclature-up-to-date/prokariotic-nomenclature-up-to-date.html">Prokaryotic Nomenclature Up to Date</a> is an alphabetical list of <i>all</i> bacterial and archaeal species names | + | <li><a href="http://www.dsmz.de/bacterial-diversity/prokaryotic-nomenclature-up-to-date/prokariotic-nomenclature-up-to-date.html">Prokaryotic Nomenclature Up to Date</a> is an alphabetical list of <i>all</i> bacterial and archaeal species names in the scientific literature. Find your species in the list, and click on its name to view details. In the table row "Type strain", look for a blue DSM link. If one is present, it will bring you to a DSMZ catalogue page where you can find details about that species. The Risk Group is listed in the table. (If there is no blue DSM link, it means that the DSMZ does not offer that species.) <br /><i>Note: "Prokaryotic Nomenclature Up to Date" has information about wild strains of microorganisms, not lab strains. Wild strains of E. coli can cause disease in humans (so they are Risk Group 2), but lab strain E. coli is Risk Group 1 and is safe to handle.</i></li> |
</ul> | </ul> | ||
<h4>Other sources</h4> | <h4>Other sources</h4> | ||
− | <p>If you cannot find your species in any of the recommended sources, you must find another reliable source. There may be a paper in the peer-reviewed scientific literature that describes your species. Your country's government might have | + | <p>If you cannot find your species in any of the recommended sources, you must find another reliable source. There may be a paper in the peer-reviewed scientific literature that describes your species. Your country's government might have its own list of microorganisms and their Risk Groups.</p> |
<p>For help finding a reliable source, you can consult your advisor, or speak to the biosafety authorities of your university. You can also contact <span class="email"><safety AT igem DOT org></span> for help.</p> | <p>For help finding a reliable source, you can consult your advisor, or speak to the biosafety authorities of your university. You can also contact <span class="email"><safety AT igem DOT org></span> for help.</p> | ||
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Revision as of 20:02, 2 February 2015
What are Risk Groups and Safety Levels?
Microorganisms are classified into four Risk Groups, and biological laboratories are classified into four corresponding Safety Levels. Risk Group 1 contains non-pathogenic organisms like yeast and E. coli K-12. The majority of iGEM teams use only Risk Group 1 organisms. Some teams use Risk Group 2 organisms. iGEM teams are not permitted to use Risk Group 3 or 4 organisms, or to work in Safety Level 3 or 4 laboratories.
Animals and plants have no Risk Group
The four Risk Groups only apply to microorganisms (bacteria, fungi, and viruses). For animals, plants, and other multicellular organisms, there are different safety guidelines. Please consult with your advisor or university authorities if you plan to do experiments with animals and plants.
If you are using a part from an animal or plant, you should consider the function of the part, and consider whether it might be dangerous to humans. For example, the gene for Green Fluorescent Protein (GFP) comes from the jellyfish Aequorea victoria, which does not have a Risk Group. GFP is only a fluorescent protein, absorbing and emitting light, so it is safe for humans and you can use it in a Safety Level 1 lab. But if you consider a different gene from A. victoria, such as the gene for the toxin in its stinging tentacles, you might conclude that you should only use that gene under strict safety conditions!
How can I find out the Risk Group of my organism?
1. Common iGEM Organisms
Species | Risk Group |
---|---|
E. coli K-12 and derivatives (DH5alpha, TOP10, etc.) | 1 |
S. cerevisiae | 1 |
Most human and mammal cell lines | 2 |
Lactobacillus spp. | 1 |
Bacillus subtilis | 1 |
2. Other Reliable Sources
To determine the Risk Group of an organism, we must consult reliable sources. There is no world-wide standard list of microorganisms and their Risk Groups. Some countries and some cell culture vendors have provided lists, but no single list includes all species, so you might need to check several sources. iGEM recommends three sources for Risk Group information: Canadian PSDS, NIH Guidelines, and DSMZ.
Canadian Pathogen Safety Data Sheets (PSDS)
The Public Health Agency of Canada has published safety data sheets for a wide variety of microorganisms.
To use the PSDS, find your species in the list. Click on the species name to open extensive safety information about that species. Scroll down to "SECTION VII" and look for "RISK GROUP CLASSIFICATION" or "CONTAINMENT REQUIREMENTS".
NIH Guidelines, Appendix B
The NIH Guidelines are a set of rules that govern research on recombinant/synthetic DNA in the United States. Appendix B of the guidelines gives a list of pathogens in Risk Groups 2, 3, and 4. It does not list Risk Group 1 organisms.
Click here to go directly to Appendix B.
DSMZ
"Always check your strain!"
A note about E. coli K-12
Escherichia coli is the most commonly used bacterium in molecular biology. E. coli bacteria naturally live inside the human digestive system. We scientists are used to thinking of E. coli as "safe", but many strains of E. coli cause terrible diseases! Famous strains such as E. coli O157:H7 have caused many deaths. Most disease-causing strains of E. coli are considered Risk Group 2.
E. coli K-12 is a "lab strain". The K-12 strain cannot survive in the human digestive system, and does not produce toxins. K-12 is Risk Group 1, and it may be used in Level 1 laboratories with the most basic safety precautions, such as rubber gloves. Similarly, the strains derived from K-12 (such as DH5alpha, TOP10, etc.) are also Risk Group 1.
Always check your strain!
DSMZ (Deutsche Sammlung von Mikroorganismen und Zellkulturen / German Collection of Microorganisms and Cell Cultures) is a large collection that includes Risk Group information for thousands of different species, strains, and cell lines. It is more difficult to use, but it includes the most species of any source that iGEM recommends.
- Catalogue Microorganisms -- search bacteria. Enter your genus, species, and/or strain information in the "Search term" box, and click Search. You will receive a long list of results, which may include many different strains and species. Examine the list to find the correct strain, and click on its DSM number to view its catalogue page. The Risk Group is listed in the table.
- Catalogue Human and Animal Cell Lines -- search this catalogue by the same method as for Catalogue Microorganisms.
- Prokaryotic Nomenclature Up to Date is an alphabetical list of all bacterial and archaeal species names in the scientific literature. Find your species in the list, and click on its name to view details. In the table row "Type strain", look for a blue DSM link. If one is present, it will bring you to a DSMZ catalogue page where you can find details about that species. The Risk Group is listed in the table. (If there is no blue DSM link, it means that the DSMZ does not offer that species.)
Note: "Prokaryotic Nomenclature Up to Date" has information about wild strains of microorganisms, not lab strains. Wild strains of E. coli can cause disease in humans (so they are Risk Group 2), but lab strain E. coli is Risk Group 1 and is safe to handle.
Other sources
If you cannot find your species in any of the recommended sources, you must find another reliable source. There may be a paper in the peer-reviewed scientific literature that describes your species. Your country's government might have its own list of microorganisms and their Risk Groups.
For help finding a reliable source, you can consult your advisor, or speak to the biosafety authorities of your university. You can also contact <safety AT igem DOT org> for help.