Difference between revisions of "Team:Nankai/Description"

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<h4>Project Description</h4>
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Poly-γ-glutamic acid (γ-PGA) is an important, naturally occurring polyamide consisting of D/L-glutamate monomers. Unlike  typical peptide linkages, the amide linkages inγ-PGA are  formed between the α-amino group and the  γ-carboxyl group. γ-PGA exhibits many favorable features such as biodegradable, water soluble, edible and non-toxic to humans and the environment. Therefore, it has been widely used in fields of foods, medicines, cosmetics and  agriculture and many unique applications, such as a sustained release material  and drug carrier, curable biological adhesive, biodegradable fibres, and highly  water absorbable hydrogels.<br>
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  Strains capable for producing γ-PGA  are divided into two categories based on their requirement for glutamate acid:  glutamate-dependent strains and glutamate-independent strains. Glutamate-independent strains are preferable for industrial production because  of their low cost and simplified fermentation process. However, compared with  glutamate-dependent strains, their lower γ-PGA productivity limits their  industrial application. Therefore,  the construction of a glutamate-independent strain with high γ-PGA yield is  important for industrial applications. <br>
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  <em>Bacillusamyloliquefaciens</em> LL3, isolated from fermented food, is a  glutamate-independent strain, which can produce 3-4 g/L γ-PGA with sucrose as  its carbon source and ammonium sulfate as its nitrogen source. The <em>B. amyloliquefaciens</em> LL3 strain was deposited in the China Center for Type Culture Collection (CCTCC) with accession number CCTCC M 208109 and its whole genome has been sequenced in 2011. In this study, we aimed to improve the γ-PGA production based on the <em>B. amyloliquefaciens </em>NK-1 strain (a derivative of LL3 strain with  its endogenous plasmid and <em>upp</em> gene  deleted).<br>
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In order to improve  γ-PGA production, we employed two strategies to fine-tune the synthetic  pathways and balance the metabolism in the glutamate-independent <em>B. amyloliquefaciens </em>NK-1 strain. Firstly,  we constructed a metabolic toggle switch in the NK-1 strain to inhibit the  expression of ODHC (2-oxoglutarate dehydrogenase complex) by adding IPTG in the  stationary stage and distribute the metabolic flux more frequently to be used  for γ-PGA precursor-glutamate synthesis. As scientists had found that the activity of ODHC was rather  low when glutamate was highly produced in a <em>Corynebacterium&nbsp;glutamicum</em> strain. Second, to balance the increase  of endogenous glutamate production, we optimized the expression level of <em>pgsBCA</em> genes (responsible for γ-PGA synthesis) by replacing its native promoter to  seven different strength of promoters. Through these two strategies, we aimed  to obtain a γ-PGA production improved mutant strain. </p>
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<span class="meta-date"><a href="#">28 February 2084</a></span>
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<h3><a href="blog-single.html">Getting Creative With the Google Maps API</a></h3>
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28 February 2084 14 Comments Candy Sharp

Getting Creative With the Google Maps API

Sed consequat lobortis risus, in rutrum arcu tristique eget. Etiam accumsan lectus quis cursus porta. Etiam a turpis sed sapien malesuada pellentesque quis id tortor. Phasellus mattis quam enim, non accumsan nibh tincidunt sed.

Nunc ullamcorper nisi sit amet eros dictum, eget vulputate quam rhoncus. Nulla diam eros, ultrices id lacinia ut, aliquet sit amet erat. Duis ut nulla molestie, ullamcorper ligula eu, ultricies tortor. Suspendisse quis consequat a turpis sed sapien quis id tortor Continue Reading...

24 February 2084 32 Comments Candy Sharp

Bird Profile Wellington New Zealand

Sed consequat lobortis risus, in rutrum arcu tristique eget. Etiam accumsan lectus quis cursus porta. Etiam a turpis sed sapien malesuada pellentesque quis id tortor. Phasellus mattis quam enim, non accumsan nibh tincidunt sed.

Nunc ullamcorper nisi sit amet eros dictum, eget vulputate quam rhoncus. Nulla diam eros, ultrices id lacinia ut, aliquet sit amet erat. Duis ut nulla molestie, ullamcorper ligula eu, ultricies tortor. Suspendisse quis consequat a turpis sed sapien quis id tortor Continue Reading...

20 February 2084 64 Comments Candy Sharp

Standard Blog Post Formating Medigo

Sed consequat lobortis risus, in rutrum arcu tristique eget. Etiam accumsan lectus quis cursus porta. Etiam a turpis sed sapien malesuada pellentesque quis id tortor. Phasellus mattis quam enim, non accumsan nibh tincidunt sed.

Nunc ullamcorper nisi sit amet eros dictum, eget vulputate quam rhoncus. Nulla diam eros, ultrices id lacinia ut, aliquet sit amet erat. Duis ut nulla molestie, ullamcorper ligula eu, ultricies tortor. Suspendisse quis consequat a turpis sed sapien quis id tortor Continue Reading...

24 February 2084 128 Comments Candy Sharp

Hochbunker High Definition Photography

Sed consequat lobortis risus, in rutrum arcu tristique eget. Etiam accumsan lectus quis cursus porta. Etiam a turpis sed sapien malesuada pellentesque quis id tortor. Phasellus mattis quam enim, non accumsan nibh tincidunt sed.

Nunc ullamcorper nisi sit amet eros dictum, eget vulputate quam rhoncus. Nulla diam eros, ultrices id lacinia ut, aliquet sit amet erat. Duis ut nulla molestie, ullamcorper ligula eu, ultricies tortor. Suspendisse quis consequat a turpis sed sapien quis id tortor Continue Reading...

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