Difference between revisions of "Team:Aalto-Helsinki/LabBook"

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<h2 id="week5">Week 5</h2>
 
<h2 id="week5">Week 5</h2>
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 +
 +
 
<h2 id="week6">Week 6</h2>
 
<h2 id="week6">Week 6</h2>
 +
 +
<table >
 +
  <tr>
 +
    <td class="bottomline rightline first"><h3 style="padding-top:0;margin-top:0;margin-bottom:0;padding-bottom:0;">Construct <br>worked with</h3></td>
 +
    <td class="bottomline"><h3>Things done</h3></td>
 +
    <td class="bottomline"></td>
 +
    <td class="bottomline"></td>
 +
    <td class="bottomline"></td>
 +
    <td class="bottomline"></td>
 +
    <td class="bottomline"></td>
 +
    <td class="bottomline"><h3>Week 6</h3></td>
 +
  </tr>
 +
  <tr>
 +
        <td class="first rightline"><div class="heightdiv"><h3>Assembly <br/> Testing RBS's</h3></div></td>
 +
        <td class="white arrow" >
 +
            <p>Re-assembling AH016 & AH018</p>
 +
        </td>
 +
        <td class="white arrow" >
 +
            <p>Transformation <br/>into BL21(DE3)</p>
 +
        </td>
 +
        <td class="red arrow" >
 +
            <p>No colonies</p>
 +
        </td>
 +
  </tr>
 +
  <tr>
 +
        <td class="first rightline"><div class="heightdiv"><h3>Testing RBS's</h3></div></td>
 +
        <td class="white arrow" >
 +
            <p>o/n cultures of <br/>nbsp;nbsp;AH016 & AH018 <br/> in TOP10</p>
 +
        </td>
 +
        <td class="blue arrow" >
 +
            <p>MiniPrep</p>
 +
        </td>
 +
        <td class="white arrow" >
 +
            <p>Transform <br/>into BL21(DE3)</p>
 +
        </td>
 +
<td class="white arrow" >
 +
            <p>Plates in rt <br/> over the <br/>weekend</p>
 +
        </td>
 +
  </tr>
 +
  <tr>
 +
        <td class="first rightline"><div class="heightdiv"><h3>Measurement <br/> Testing RBS's</h3></div></td>
 +
        <td class="white arrow" >
 +
            <p>AH018 in <br/>BL21(DE3)</p>
 +
        </td>
 +
        <td class="white arrow" >
 +
            <p >o/n culture</p>
 +
        </td>
 +
        <td class="white arrow" >
 +
            <p style="font-size:12px">IPTG induction & <br/>blue chromoprotein </br>measurement</p>
 +
        </td>
 +
<td class="red arrow" >
 +
            <p>No blue chromoprotein</p>
 +
        </td>
 +
  </tr>
 +
    <tr>
 +
        <td class="first rightline"><div class="heightdiv"><h3></h3></div></td>
 +
        <td>
 +
        </td>
 +
        <td class="white arrow" >
 +
            <p >Refresh on IPTG plate</p>
 +
        </td>
 +
        <td class="red arrow" >
 +
            <p>No blue chromoprotein</p>
 +
        </td>
 +
  </tr>
 +
 +
  </tr>
 +
    <tr>
 +
        <td class="first rightline"><div class="heightdiv"><h3>Producing linearized backbones</h3></div></td>
 +
 +
        <td class="white arrow" >
 +
            <p >o/n cultures of <br/>AH007-AH009</p>
 +
        </td>
 +
 +
        <td class="blue arrow" >
 +
            <p>MiniPrep</p>
 +
        </td>
 +
 +
        <td class="blue arrow" >
 +
            <p>Restrict & <br/>extract <br/>from gel</p>
 +
        </td>
 +
  </tr>
 +
</table>
 +
 
<h2 id="week7">Week 7</h2>
 
<h2 id="week7">Week 7</h2>
 
<h2 id="week8">Week 8</h2>
 
<h2 id="week8">Week 8</h2>

Revision as of 14:46, 6 August 2015

Lab Book

An example of what the table could look like:

Construct
worked with

Things done

Lab 1.1.2015
Monday

CAR

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text, there
is lot of it

another CAR?

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text, there
is lot of it

Ado?

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Ado cont.

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Here is my
text, there
is lot of it

Week 1

Construct
worked with

Things done

May 18th - May 22nd

Plates

Making Cm
plates

Stored in
+4°C

Competent Cells

Preparing
solutions for
competent cells

Stored in
+4°C

Competent Cells

o/n culture
of E. coli
TOP10

Chemical
competent
cells protocol

Tranformation
efficiency kit

Competent Cells

o/n culture
of E. coli
BL21(DE3)

Chemical
competent
cells protocol

Tranformation
efficiency kit

Colonies are
the wrong color

Propagations

Transformed
  AH001-AH008
into TOP10

 AH001-AH004
& AH006

Stored in
+4°C

No colonies for
   AH005, AH007
or AH008

Week 2

Construct
worked with

Things done

May 25th - May 29th

Propagations

Transformed
AH007-AH009,
AH005 & AH019
into TOP10

AH007-AH009
& AH019

o/n cultures

MiniPrep

No colonies
for AH005

Re-transform
into TOP10

Plates in rt
over weekend

Competent Cells

o/n culture
of BL21(DE3)
transformants

MiniPrep

PCR with
VF2 & VR

Smeared gels

Assembly
Testing RBS's

Restrict
AH001-AH004
& AH006-AH008

Ligation

AH002+
  AH004+AH008
-> AH014

Repeat
ligation

Restrict &
extract
from gel

Transformation
into TOP10

Plates in rt
over weekend

AH001+
   AH006+AH008
-> AH011

Repeat
ligation

Restrict &
extract
from gel

Transformation
into TOP10

Plates in rt
over weekend

AH001+
   AH003+AH008
-> AH013

Repeat
ligation

Restrict &
extract
from gel

Transformation
into TOP10

Plates in rt
over weekend

Propagations

o/n culture of
  AH001-AH004
& AH006

MiniPrep

Week 3

Construct
worked with

Things done

June 1st - June 5th

Assembly
Testing RBS's

No colonies
for AH011
or AH014

re-assemble

Restrict &
extract
from gel

Ligate &
tranform
into TOP10

No colonies

Assembly
Testing RBS's

AH005 &
AH013 OK

o/n cultures

MiniPrep

Propagations

Transform
    AH020 & AH021
into TOP10

Plates in rt
 over weekend

Week 4

Construct
worked with

Things done

June 8th - June 12th

Assembly
Propagations

o/n cultures
for AH020
& AH021

MiniPrep

Assembly
Testing RBS's

Re-assembling
   AH011, AH013
& AH014

Transform into TOP10

o/n cultures

MinPrep

Assembly
Testing RBS's

Assembling
AH015

  Ligate AH002+
  AH005+AH008

Restrict &
extract
from gel

Transform into TOP10

o/n cultures

MiniPrep

Propagations

Transform
    AH020 & AH021
into TOP10

Plates in rt
over weekend

Week 5

Week 6

Construct
worked with

Things done

Week 6

Assembly
Testing RBS's

Re-assembling AH016 & AH018

Transformation
into BL21(DE3)

No colonies

Testing RBS's

o/n cultures of
nbsp;nbsp;AH016 & AH018
in TOP10

MiniPrep

Transform
into BL21(DE3)

Plates in rt
over the
weekend

Measurement
Testing RBS's

AH018 in
BL21(DE3)

o/n culture

IPTG induction &
blue chromoprotein
measurement

No blue chromoprotein

Refresh on IPTG plate

No blue chromoprotein

Producing linearized backbones

o/n cultures of
AH007-AH009

MiniPrep

Restrict &
extract
from gel

Week 7

Week 8

Week 9

Week 10

Week 11

Week 12

Week 13

Week 14

Week 15

Week 16

Week 17

Week 18