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− | =Monday 3rd August=
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− | ==Lab Work==
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− | === New culture===
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− | '' by Pauline''
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− | Biobricks:
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− | * BBa_K1707008
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− | * BBa_I13600 (2014)
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− | 6 clones of each in 5mL LB + 20µg/mL of chloramphenicol
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− | Incubation at 37°C, 250 rpm
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− |
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− | ===Plasmid extraction===
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− | ''by Seong-Koo''
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− | Biobricks:
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− | * BBa_K1707011 #1 to #6
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− | * BBa_K1707021 #1 to #6
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− | * BBa_K1707004 #1 to #6
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− | Plasmid pIJ773
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− | With the Macherey-Nagel Extraction kit
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− | ===Soil experiment===
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− | ''by Coralie''
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− |
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− | ====Soil====
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− | Observation of plates (31/07/2015):
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− | * Soil + H2O on new MCK: lots of colonies are visible
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− | * Soil + LB on new MCK: lots of colonies are visible
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− | * LB on new MCK: nothing
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− | * MCK alone: nothing
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− | We can suppose two different hypothesis:
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− | * The soil is contaminated
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− | * the MCK isn't OK for our experiment: now we add lactose in the preparation. We will try with sorbitol instead of lactose.
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− | We try soil + H2O and soil +LB on:
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− | * MCK + Sorbitol: Without antibiotics, with Spectinomycine, Tetramycine or Chloramphenicol
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− | * MCK + Lactose: Without antibiotics, with Spectinomycine, Tetramycine or Chloramphenicol
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− | Incubation ON, 37°C
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− | '''Member present:'''
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− | * Instructors: Claire
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− | * Students: Coralie, Audrey and Pauline
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− | {{Team:Paris_Saclay/notebook_footer}}
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