Difference between revisions of "Team:Paris Saclay/Notebook/August/1"

(Blanked the page)
Line 1: Line 1:
=Monday 3rd August=
 
==Lab Work==
 
=== New culture===
 
'' by Pauline''
 
  
Biobricks:
 
* BBa_K1707008
 
* BBa_I13600 (2014)
 
 
6 clones of each in 5mL LB + 20µg/mL of chloramphenicol
 
 
Incubation at 37°C, 250 rpm
 
 
===Plasmid extraction===
 
''by Seong-Koo''
 
 
Biobricks:
 
* BBa_K1707011 #1 to #6
 
* BBa_K1707021 #1 to #6
 
* BBa_K1707004 #1 to #6
 
 
Plasmid pIJ773
 
 
With the Macherey-Nagel Extraction kit
 
 
===Soil experiment===
 
''by Coralie''
 
 
====Soil====
 
Observation of plates (31/07/2015): 
 
 
* Soil + H2O on new MCK:  lots of colonies are visible
 
* Soil + LB on new MCK: lots of colonies are visible
 
* LB on new MCK: nothing
 
* MCK alone: nothing
 
 
We can suppose two different hypothesis:
 
* The soil is contaminated
 
* the MCK isn't OK for our experiment: now we add lactose in the preparation. We will try with sorbitol instead of lactose.
 
 
We try soil + H2O and soil +LB on:
 
* MCK + Sorbitol: Without antibiotics, with Spectinomycine, Tetramycine or Chloramphenicol
 
* MCK + Lactose: Without antibiotics, with Spectinomycine, Tetramycine or Chloramphenicol
 
 
Incubation ON, 37°C
 
 
 
 
 
'''Member present:'''
 
* Instructors: Claire
 
* Students: Coralie, Audrey and Pauline
 
{{Team:Paris_Saclay/notebook_footer}}
 

Revision as of 07:37, 15 September 2015