Difference between revisions of "Team:DTU-Denmark"

Line 1: Line 1:
−
<html lang="en">
 
−
<head>
 
−
  <meta charset="utf-8">
 
−
  <meta name="viewport" content="width=device-width, initial-scale=1.0">
 
  
−
 
+
 
 +
<html>
 +
  <head>
 +
    <meta name="viewport" content="width=device-width, initial-scale=1, maximum-scale=1, user-scalable=no">
 +
    <meta http-equiv="X-UA-Compatible" content="IE=edge">
 +
   
 +
   
 
     <link href="/Team:DTU-Denmark/bootstrap.css?action=raw&ctype=text/css" rel="stylesheet">
 
     <link href="/Team:DTU-Denmark/bootstrap.css?action=raw&ctype=text/css" rel="stylesheet">
−
    <link href="/Team:DTU-Denmark/style.css?action=raw&ctype=text/css" rel="stylesheet">
 
 
     <link href="/Team:DTU-Denmark/font-awesome.css?action=raw&ctype=text/css" rel="stylesheet">
 
     <link href="/Team:DTU-Denmark/font-awesome.css?action=raw&ctype=text/css" rel="stylesheet">
 +
    <link href="/Team:DTU-Denmark/wiki.css?action=raw&ctype=text/css" rel="stylesheet">
 +
    <link href="/Team:DTU-Denmark/timeline.css?action=raw&ctype=text/css" rel="stylesheet">
  
 
      
 
      
 
     <script type="text/javascript" src="/Team:DTU-Denmark/jquery.js?action=raw&ctype=text/javascript"></script>
 
     <script type="text/javascript" src="/Team:DTU-Denmark/jquery.js?action=raw&ctype=text/javascript"></script>
 
     <script type="text/javascript" src="/Team:DTU-Denmark/bootstrap.js?action=raw&ctype=text/javascript"></script>
 
     <script type="text/javascript" src="/Team:DTU-Denmark/bootstrap.js?action=raw&ctype=text/javascript"></script>
 +
    <script type="text/javascript" src="/Team:DTU-Denmark/jquery.easing.js?action=raw&ctype=text/javascript"></script>
 
     <script type="text/javascript" src="/Team:DTU-Denmark/wiki.js?action=raw&ctype=text/javascript"></script>
 
     <script type="text/javascript" src="/Team:DTU-Denmark/wiki.js?action=raw&ctype=text/javascript"></script>
−
</head>
+
   
−
<body>
+
    <!-- HTML5 shim and Respond.js for IE8 support of HTML5 elements and media queries -->
−
<div class="container">
+
    <!-- WARNING: Respond.js doesn't work if you view the page via file:// -->
−
<div class="row clearfix">
+
    <!--[if lt IE 9]>
−
<div class="col-md-12 column">
+
      <script src="https://oss.maxcdn.com/html5shiv/3.7.2/html5shiv.min.js"></script>
−
<div class="page-header">
+
      <script src="https://oss.maxcdn.com/respond/1.4.2/respond.min.js"></script>
−
<h1>Technical University of Denmark
+
    <![endif]-->
−
<!--small>Team Name</small-->
+
   
−
</h1>
+
  </head>
−
</div>
+
  <body data-spy="scroll" data-target="#scrollspy" data-offset="90">
−
</div>
+
    <div id="fb-root"></div>
−
    <div class="col-md-2">
+
    <script>(function(d, s, id) {
−
        <div class="sidebar-nav">
+
      var js, fjs = d.getElementsByTagName(s)[0];
−
<nav class="navbar navbar-default" role="navigation">
+
      if (d.getElementById(id)) return;
−
<div class="collapse navbar-collapse" id="bs-example-navbar-collapse-1">
+
      js = d.createElement(s); js.id = id;
−
<ul class="nav navbar-nav">
+
      js.src = "//connect.facebook.net/da_DK/sdk.js#xfbml=1&version=v2.4";
−
<li class="dropdown active">
+
      fjs.parentNode.insertBefore(js, fjs);
−
<a href="/Team:DTU-Denmark"
+
    }(document, 'script', 'facebook-jssdk'));</script>
−
  >Home
+
    <nav class="navbar navbar-default navbar-fixed-top" role="navigation">
−
                                          </a></li>
+
      <!-- Brand and toggle get grouped for better mobile display -->
−
<li class="dropdown">
+
      <div class="container">
−
<a href="/Team:DTU-Denmark/Team"
+
        <div class="navbar-header">
−
  >Team
+
          <button type="button" class="navbar-toggle" data-toggle="collapse" data-target="#nav-collapse">
−
                                          </a></li>
+
            <span class="sr-only">Toggle navigation</span>
−
<li class="dropdown">
+
            <i class="fa fa-bars"></i>
−
<a href=""
+
            <!--span class="icon-bar"></span>
−
  class="dropdown-toggle"
+
            <span class="icon-bar"></span>
−
  data-toggle="dropdown"
+
            <span class="icon-bar"></span-->
−
  role="button"
+
          </button>
−
  aria-expanded="false">Project
+
          <a class="navbar-brand navimg" href="#"><img src="/wiki/images/b/b7/DTU-Denmark_dtulogo.png"></a>
−
                                          <span class="caret"></span></a><ul class="dropdown-menu" role="menu">
+
−
<li class="dropdown">
+
−
<a href="/Team:DTU-Denmark/Description"
+
−
  >Description
+
−
                                          </a></li>
+
−
<li class="dropdown">
+
−
<a href="/Team:DTU-Denmark/Experiments"
+
−
  >Experiments & Protocols
+
−
                                          </a></li>
+
−
<li class="dropdown">
+
−
<a href="/Team:DTU-Denmark/Results"
+
−
  >Results
+
−
                                          </a></li>
+
−
<li class="dropdown">
+
−
<a href="/Team:DTU-Denmark/Design"
+
−
  >Design
+
−
                                          </a></li></ul></li>
+
−
<li class="dropdown">
+
−
<a href=""
+
−
  class="dropdown-toggle"
+
−
  data-toggle="dropdown"
+
−
  role="button"
+
−
  aria-expanded="false">Parts
+
−
                                          <span class="caret"></span></a><ul class="dropdown-menu" role="menu">
+
−
<li class="dropdown">
+
−
<a href="/Team:DTU-Denmark/Parts"
+
−
  >Team Parts
+
−
                                          </a></li>
+
−
<li class="dropdown">
+
−
<a href="/Team:DTU-Denmark/Basic_Part"
+
−
  >Basic Parts
+
−
                                          </a></li>
+
−
<li class="dropdown">
+
−
<a href="/Team:DTU-Denmark/Composite_Part"
+
−
  >Composite Parts
+
−
                                          </a></li>
+
−
<li class="dropdown">
+
−
<a href="/Team:DTU-Denmark/Part_Collection"
+
−
  >Part Collection
+
−
                                          </a></li></ul></li>
+
−
<li class="dropdown">
+
−
<a href="/Team:DTU-Denmark/Notebook"
+
−
  >Notebook
+
−
                                          </a></li>
+
−
<li class="dropdown">
+
−
<a href="/Team:DTU-Denmark/Attributions"
+
−
  >Attributions
+
−
                                          </a></li>
+
−
<li class="dropdown">
+
−
<a href="/Team:DTU-Denmark/Collaborations"
+
−
  >Collaborations
+
−
                                          </a></li>
+
−
<li class="dropdown">
+
−
<a href="/Team:DTU-Denmark/Practices"
+
−
  >Human Practices
+
−
                                          </a></li>
+
−
<li class="dropdown">
+
−
<a href="/Team:DTU-Denmark/Safety"
+
−
  >Safety
+
−
                                          </a></li>
+
−
<li class="dropdown">
+
−
<a href="/Team:DTU-Denmark/Modeling"
+
−
  >Modeling
+
−
                                          </a></li>
+
−
<li class="dropdown">
+
−
<a href="/Team:DTU-Denmark/Measurement"
+
−
  >Measurement
+
−
                                          </a></li>
+
−
<li class="dropdown">
+
−
<a href="/Team:DTU-Denmark/Software"
+
−
  >Software
+
−
                                          </a></li>
+
−
<li class="dropdown">
+
−
<a href="/Team:DTU-Denmark/Entrepreneurship"
+
−
  >Entrepreneurship
+
−
                                          </a></li>
+
−
</ul>
+
−
</div>
+
−
</nav>
+
−
            </div>
+
 
         </div>
 
         </div>
−
    <div class="col-md-9">
+
 
−
             <div class="row clearfix">
+
        <!-- Collect the nav links, forms, and other content for toggling -->
−
+
        <div class="collapse navbar-collapse" id="nav-collapse">
−
+
          <ul class="nav navbar-nav navbar-right hidden-lg hidden-xs">
−
<div class="row col-md-12 column">
+
            <li>
−
    <h2>
+
              <a href="https://igem.org" target="_blank" class="navimg">
 +
                <img src="/wiki/images/a/af/DTU-Denmark_igemlogo.png">
 +
              </a>
 +
            </li>
 +
          </ul>
 +
          <ul class="nav navbar-nav navbar-right hidden-xs">
 +
            <li>
 +
              <a href="http://facebook.com/dtubiobuilders" data-toggle="tooltip" data-placement="bottom" title="Follow us on Facebook">
 +
                <i class="fa fa-facebook"></i>
 +
              </a>
 +
            </li>
 +
            <li>
 +
              <a href="https://twitter.com/IGEM_DTU" data-toggle="tooltip" data-placement="bottom" title="Follow us on Twitter">
 +
                <i class="fa fa-twitter"></i>
 +
              </a>
 +
            </li>
 +
          </ul>
 +
          <ul class="nav nav-justified">
 +
           
 +
            <li  class="active" >
 +
              <a class="active" href="/Team:DTU-Denmark">Home</a>
 +
            </li>
 +
           
 +
            <li >
 +
              <a class="active" href="/Team:DTU-Denmark/Team">Team</a>
 +
            </li>
 +
           
 +
            <li >
 +
              <a class="active" href="/Team:DTU-Denmark/Project">Project</a>
 +
            </li>
 +
           
 +
            <li >
 +
              <a class="active" href="/Team:DTU-Denmark/Practices">Human Practices</a>
 +
            </li>
 +
           
 +
            <li >
 +
              <a class="active" href="/Team:DTU-Denmark/Collaborations">Collaborations</a>
 +
            </li>
 +
           
 +
            <li >
 +
              <a class="active" href="/Team:DTU-Denmark/Journal">Journal</a>
 +
            </li>
 +
           
 +
          </ul>
 +
          <ul class="nav navbar-nav navbar-right visible-xs">
 +
            <li>
 +
              <a href="https://igem.org" target="_blank" class="navimg">
 +
                <img src="/wiki/images/a/af/DTU-Denmark_igemlogo.png">
 +
              </a>
 +
            </li>
 +
            <li><a href="http://facebook.com/dtubiobuilders"><i class="fa fa-facebook-square"></i> Follow us on Facebook</a></li>
 +
            <li><a href="https://twitter.com/IGEM_DTU"><i class="fa fa-twitter-square"></i> Follow us on Twitter</a></li>
 +
          </ul>
 +
        </div><!-- /.navbar-collapse -->
 +
      </div>
 +
    </nav>
 +
    <nav class="navbar navbar-default extrabar hidden-xs hidden" data-spy="affix">
 +
      <div class="container" id="scrollspy">
 +
        <ul class="nav nav-justified">
 +
          <li><a class="page-scroll" href="#Project-description">Project description</a></li><li><a class="page-scroll" href="#Social-Media">Social Media</a></li>
 +
        </ul>
 +
      </div>
 +
    </nav>
 +
    <div class="jumbotron hero bg-hero">
 +
      <div class="igem-logo visible-lg">
 +
        <a href="https://igem.org" target="_blank">
 +
          <img src="/wiki/images/a/af/DTU-Denmark_igemlogo.png">
 +
        </a>
 +
      </div>
 +
      <div class="container">
 +
        <div class="row hero-text">
 +
          <div class="col-md-12">
 +
              
 +
            <h3>Technical University of Denmark</h3>
 +
            <h1>The Synthesizer</h1>
 +
           
 +
            <hr/>
 +
            <ul class="list-inline hidden-xs hidden-sm">
 +
             
 +
              <li>
 +
                <a id="Project-description-submenu" class="btn btn-default btn-transparent page-scroll" href="#Project-description">Project description</a>
 +
              </li>
 +
             
 +
              <li>
 +
                <a id="Social-Media-submenu" class="btn btn-default btn-transparent page-scroll" href="#Social-Media">Social Media</a>
 +
              </li>
 +
             
 +
            </ul>
 +
            <ul class="list-inline visible-xs visible-sm">
 +
             
 +
              <li>
 +
                <a id="Project-description-submenu" class="page-scroll" href="#Project-description">Project description</a>
 +
              </li>
 +
             
 +
              <li>
 +
                <a id="Social-Media-submenu" class="page-scroll" href="#Social-Media">Social Media</a>
 +
              </li>
 +
             
 +
            </ul>
 +
          </div>
 +
        </div>
 +
        <div class="row">
 +
          <div class="col-md-12">
 +
            Scroll down for more<br>
 +
            <a href="#Project-description" class="btn btn-circle btn-transparent page-scroll">
 +
              <i class="fa fa-angle-double-down"></i>
 +
            </a>
 +
          </div>
 +
        </div>
 +
      </div>
 +
    </div>
 +
   
 +
   
 +
   
 +
<div id="Project-description">
 +
  <div class="container">
 +
    <div class="row col-md-12">
 +
      <h2>
 
         Project description
 
         Project description
−
    </h2>
+
      </h2>
−
    <p>Nonribosomal peptides, polyketides and hybrids thereof are secondary metabolites that are biosynthesized by condensation of amino and/or carboxylic acids. These metabolites have a wide variety of important biological activities that include antibacterial, anticancer and immunosuppressing activities.</p>
+
      <p>Non-ribosomal peptides have important anti-bacterial, anti-cancer, and immunosuppressive biological activities. They are synthesized by modular, high molecular weight enzymes that assemble more than 500 different amino acid substrates in an assembly line manner. For this reason, synthetic biologists have tried to engineer these proteins and to switch modules to create analogs and novel natural products, but with little success. Despite being modular, the interactions between modules have evolved to be highly specific, making synthetic Non-Ribosomal Peptide Synthases (NRPS) a challenge to engineer. Instead of switching modules we introduced a recombination system targeting oligo integration in Bacillus subtilis. We used the recombineering system to alter the active sites determining substrate specificity, thereby creating variants of antibiotics. Our focus was the tyrocidine antibiotic, which cannot be used intravenously due to its toxicity. Our goal is to create new analogs through multiplex automated genome engineering to reduce toxicity.</p>
  
−
<p>New ways to rationally design such products and creation of libraries that can be tested for desired activities can lead to new medicinally and industrially important products.</p>
+
    </div>
−
 
+
  </div>
−
<p>Nonribosomal peptide synthetases(nrps) and polyketide synthases(pks) consist of many modules that incorporate one amino or carboxylic acid unto a growing chain. These modules can be rearranged and their specificity can be altered in order to make new products. The aim of our project is to design modules that can be combined in order to create nrps/pks hybrids as well as an easy way to create libraries of similar compounds with altered side chains based on an existing scaffold.</p>
+
</div>
 +
   
 +
   
 +
   
 +
<div id="Social-Media">
 +
  <div class="container">
 +
    <div class="row col-md-12">
 +
      <h2>
 +
        Social Media
 +
      </h2>
 +
      <div>
 +
<p><a class="twitter-timeline" data-widget-id="631150747221139456" href="https://twitter.com/iGEM_DTU">Tweets by @iGEM_DTU</a> <script>!function(d,s,id){var js,fjs=d.getElementsByTagName(s)[0],p=/^http:/.test(d.location)?'http':'https';if(!d.getElementById(id)){js=d.createElement(s);js.id=id;js.src=p+"://platform.twitter.com/widgets.js";fjs.parentNode.insertBefore(js,fjs);}}(document,"script","twitter-wjs");</script></p>
  
 +
<div class="fb-page" data-adapt-container-width="true" data-height="600" data-hide-cover="false" data-href="https://www.facebook.com/dtubiobuilders" data-show-facepile="true" data-show-posts="true" data-small-header="false">
 +
<div class="fb-xfbml-parse-ignore">
 +
<blockquote cite="https://www.facebook.com/dtubiobuilders"><a href="https://www.facebook.com/dtubiobuilders">DTU Biobuilders</a></blockquote>
 
</div>
 
</div>
−
 
−
</div>
 
−
</div>
 
−
</div>
 
 
</div>
 
</div>
−
</body>
+
</div>
 +
 
 +
<p>&nbsp;</p>
 +
 
 +
<p>&nbsp;</p>
 +
 
 +
<p>&nbsp;</p>
 +
 
 +
    </div>
 +
  </div>
 +
</div>
 +
   
 +
    <footer class="container">
 +
      <div class="row">
 +
        <div class="col-md-2 col-md-offset-2">
 +
          <a href="http://www.dtu.dk" target="_blank">
 +
            <img src="/wiki/images/b/b7/DTU-Denmark_dtulogo.png" height="130px">
 +
          </a>
 +
        </div>
 +
        <div class="col-md-4">
 +
          <address>
 +
            <strong>Technical University of Denmark</strong><br>
 +
            Department of Systems Biology <br>
 +
            Søltofts Plads 221 <br>
 +
            2800 Kgs. Lyngby<br>
 +
            Denmark<br>
 +
            <abbr title="Phone">P:</abbr> +45 45 25 25 25<br>
 +
            <abbr title="Mail">M:</abbr> <a href="mailto:dtu-igem-2015@googlegroups.com">dtu-igem-2015@googlegroups.com</a>
 +
          </address>
 +
        </div>
 +
        <div class="col-md-2">
 +
          <a href="https://igem.org" style="float:left" target="_blank">
 +
              <img src="/wiki/images/a/af/DTU-Denmark_igemlogo.png" height="90px">
 +
          </a>
 +
        </div>
 +
      </div>
 +
    </footer>
 +
   
 +
  </body>
 
</html>
 
</html>

Revision as of 10:10, 18 August 2015


Technical University of Denmark

The Synthesizer

Scroll down for more

Project description

Non-ribosomal peptides have important anti-bacterial, anti-cancer, and immunosuppressive biological activities. They are synthesized by modular, high molecular weight enzymes that assemble more than 500 different amino acid substrates in an assembly line manner. For this reason, synthetic biologists have tried to engineer these proteins and to switch modules to create analogs and novel natural products, but with little success. Despite being modular, the interactions between modules have evolved to be highly specific, making synthetic Non-Ribosomal Peptide Synthases (NRPS) a challenge to engineer. Instead of switching modules we introduced a recombination system targeting oligo integration in Bacillus subtilis. We used the recombineering system to alter the active sites determining substrate specificity, thereby creating variants of antibiotics. Our focus was the tyrocidine antibiotic, which cannot be used intravenously due to its toxicity. Our goal is to create new analogs through multiplex automated genome engineering to reduce toxicity.

Social Media

DTU Biobuilders

 

 

 

Technical University of Denmark
Department of Systems Biology
Søltofts Plads 221
2800 Kgs. Lyngby
Denmark
P: +45 45 25 25 25
M: dtu-igem-2015@googlegroups.com