Difference between revisions of "Team:Aalto-Helsinki/InterLabBook"

m (Week 1 & 2)
m (week3&4)
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</table>
 
</table>
 +
 
<h2 id="week2">Week 2</h2>
 
<h2 id="week2">Week 2</h2>
 +
 
<table >
 
<table >
 
   <tr>
 
   <tr>
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         </td>
 
         </td>
 
<td class="white arrow" >
 
<td class="white arrow" >
−
             <p>Re-assemble D1-D3</p>
+
             <p>Re-assemble <br/>D1-D3</p>
 
         </td>
 
         </td>
 
<td class="white arrow" >
 
<td class="white arrow" >
−
             <p>Transform into TOP10</p>
+
             <p>Transform <br/>into TOP10</p>
 
         </td>
 
         </td>
 
<td class="blue arrow" >
 
<td class="blue arrow" >
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<h2 id="week3">Week 3</h2>
 
<h2 id="week3">Week 3</h2>
 +
 +
<table>
 +
  <tr>
 +
    <td class="bottomline rightline first"><h3 style="padding-top:0;margin-top:0;margin-bottom:0;padding-bottom:0;">Construct <br>worked with</h3></td>
 +
    <td class="bottomline"><h3>Things done</h3></td>
 +
    <td class="bottomline"></td>
 +
    <td class="bottomline"></td>
 +
    <td class="bottomline"></td>
 +
    <td class="bottomline"></td>
 +
    <td class="bottomline"></td>
 +
    <td class="bottomline"><h3>July 6th - <br/> July 10th</h3></td>
 +
  </tr>
 +
  <tr>
 +
        <td class="first rightline"><div class="heightdiv"><h3>Checking D2</h3></div></td>
 +
        <td class="white arrow" >
 +
            <p>Transform <br/> D2 <br/>into TOP10</p>
 +
        </td>
 +
        <td class="blue arrow" >
 +
            <p>GFP <br/> expressed</p>
 +
        </td>
 +
  </tr>
 +
 +
</table>
 +
 +
 
<h2 id="week4">Week 4</h2>
 
<h2 id="week4">Week 4</h2>
 +
 +
<table>
 +
  <tr>
 +
    <td class="bottomline rightline first"><h3 style="padding-top:0;margin-top:0;margin-bottom:0;padding-bottom:0;">Construct <br>worked with</h3></td>
 +
    <td class="bottomline"><h3>Things done</h3></td>
 +
    <td class="bottomline"></td>
 +
    <td class="bottomline"></td>
 +
    <td class="bottomline"></td>
 +
    <td class="bottomline"></td>
 +
    <td class="bottomline"></td>
 +
    <td class="bottomline"><h3>July 20th - <br/> July 24th</h3></td>
 +
  </tr>
 +
  <tr>
 +
        <td class="first rightline"><div class="heightdiv"><h3>Assembly</h3></div></td>
 +
        <td class="white arrow" >
 +
            <p>Re-restrict <br/> AHD1 & AHD3-AHD5</p>
 +
        </td>
 +
        <td class="white arrow" >
 +
            <p>Stored in -20°C</p>
 +
        </td>
 +
  </tr>
 +
 +
</table>
 +
 
<h2 id="week5">Week 5</h2>
 
<h2 id="week5">Week 5</h2>
 +
 +
<table>
 +
  <tr>
 +
    <td class="bottomline rightline first"><h3 style="padding-top:0;margin-top:0;margin-bottom:0;padding-bottom:0;">Construct <br>worked with</h3></td>
 +
    <td class="bottomline"><h3>Things done</h3></td>
 +
    <td class="bottomline"></td>
 +
    <td class="bottomline"></td>
 +
    <td class="bottomline"></td>
 +
    <td class="bottomline"></td>
 +
    <td class="bottomline"></td>
 +
    <td class="bottomline"><h3>July 27th - <br/> July 31st</h3></td>
 +
  </tr>
 +
  <tr>
 +
        <td class="first rightline"><div class="heightdiv"><h3>Assembly</h3></div></td>
 +
        <td class="white arrow" >
 +
            <p>Ligations <br/>for D1 & D3</p>
 +
        </td>
 +
        <td class="red arrow" >
 +
            <p>Transform <br/> into TOP10</p>
 +
        </td>
 +
  </tr>
 +
  <tr>
 +
        <td class="first rightline"><div class="heightdiv"><h3>Checking</h3></div></td>
 +
        <td class="white arrow" >
 +
            <p>Check AHD1 <br/>& AHD3-AHD5 <br/> restrictions on gel</p>
 +
        </td>
 +
        <td class="blue arrow" >
 +
            <p>AHD4 & AHD5 <br/> OK</p>
 +
        </td>
 +
        <td class="white arrow" >
 +
            <p>Too little <br/>restriction mix<br/> for new ligation</p>
 +
        </td>
 +
        <td class="white arrow" >
 +
            <p>Re-restrict</p>
 +
        </td>
 +
        <td class="white arrow" >
 +
            <p>Stored in -20°C</p>
 +
        </td>
 +
  </tr>
 +
  <tr>
 +
        <td class="first rightline"><div class="heightdiv"><h3>Assembly</h3></div></td>
 +
        <td class="white arrow" >
 +
            <p>Ligations <br/>for D1 & D3</p>
 +
        </td>
 +
        <td class="red arrow" >
 +
            <p>Transform <br/> into TOP10</p>
 +
        </td>
 +
  </tr>
 +
  <tr>
 +
        <td class="first rightline"><div class="heightdiv"><h3></h3></div></td>
 +
        <td>
 +
        </td>
 +
        <td class="red arrow" >
 +
            <p>AHD1 & AHD3 <br/> unclear</p>
 +
        </td>
 +
        <td class="white arrow" >
 +
            <p>Re-restrict</p>
 +
        </td>
 +
        <td class="white arrow" >
 +
            <p>Stored <br/>in -20°C</p>
 +
        </td>
 +
  </tr>
 +
 +
</table>
 +
 
<h2 id="week6">Week 6</h2>
 
<h2 id="week6">Week 6</h2>
 
<h2 id="week7">Week 7</h2>
 
<h2 id="week7">Week 7</h2>

Revision as of 12:44, 12 August 2015

Lab Book

An example of what the table could look like:

Construct
worked with

Things done

Lab 1.1.2015
Monday

CAR

Here is my text

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Here is my text

Here is my
text, there
is lot of it

another CAR?

Here is my text

Here is my text

Here is my text

Here is my
text, there
is lot of it

Ado?

Here is my text

Here is my text

Here is my text

Here is my text

Here is my text

Ado cont.

Here is my text

Here is my text

Here is my text

Here is my text

Here is my
text, there
is lot of it

Week 1

Construct
worked with

Things done

June 22nd -
June 26th

Preparations

Transform
AHD1-AHD4
into TOP10

o/n culture

MinPrep

Use iGEM's
linearized
backbones

Assembly

3A assembly
for D1-D3

Stored
in -20°C

Week 2

Construct
worked with

Things done

June 29th -
July 7th

Preparations

Transform
AHD6 & AHD7
into TOP10

o/n culture

MinPrep

Assembly

Transform
D1-D3
into TOP10

D2
expressing
GFP

Refresh
positive
colonies

No GFP

No colonies
for D1 & D3

Re-assemble
D1-D3

Transform
into TOP10

D2
successful

o/n culture

MiniPrep

No colonies
for D1 & D3

Week 3

Construct
worked with

Things done

July 6th -
July 10th

Checking D2

Transform
D2
into TOP10

GFP
expressed

Week 4

Construct
worked with

Things done

July 20th -
July 24th

Assembly

Re-restrict
AHD1 & AHD3-AHD5

Stored in -20°C

Week 5

Construct
worked with

Things done

July 27th -
July 31st

Assembly

Ligations
for D1 & D3

Transform
into TOP10

Checking

Check AHD1
& AHD3-AHD5
restrictions on gel

AHD4 & AHD5
OK

Too little
restriction mix
for new ligation

Re-restrict

Stored in -20°C

Assembly

Ligations
for D1 & D3

Transform
into TOP10

AHD1 & AHD3
unclear

Re-restrict

Stored
in -20°C

Week 6

Week 7

Week 8

Week 9

Week 10

Week 11

Week 12

Week 13

Week 14

Week 15

Week 16

Week 17

Week 18