Difference between revisions of "Team:UCLA/Notebook/Spider Silk Genetics/12 August 2015"

(→‎Colony PCR for M1 SwqAB 1 & 2 and M1-9(T7))
Line 20: Line 20:
 
| ddH2O
 
| ddH2O
 
| 4 uL
 
| 4 uL
 +
|}
 +
 +
{| class="wikitable"
 +
| 95C
 +
| 3 min
 +
|-
 +
| 95C
 +
| 30 sec
 +
|-
 +
| 72C
 +
| 25 sec (M1SeqAB)/ 1min 40 sec (M1-9)
 +
|-
 +
|
 +
| x30
 +
|-
 +
| 72C
 +
| 5 min
 +
|-
 +
| 12C
 +
| Hold
 
|}
 
|}

Revision as of 06:15, 13 August 2015

iGEM UCLA




08/12/2015

Colony PCR for M1 SwqAB 1 & 2 and M1-9(T7)

2X Taq MM 6.25 uL
VF Primer 0.625 uL
VR Primer 0.625 uL
Template 1 uL
ddH2O 4 uL
95C 3 min
95C 30 sec
72C 25 sec (M1SeqAB)/ 1min 40 sec (M1-9)
x30
72C 5 min
12C Hold