Difference between revisions of "Team:UiOslo Norway/Experiments/Alkaline Phospatase"

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Use Zero blunt TOPO PCR cloning Kit</br>
 
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2 μl 10X Reaction Buffer</br>
 
2 μl 10X Reaction Buffer</br>

Revision as of 08:12, 14 August 2015

Shrimp Alkaline Phosphatase Treatment (Fermentas):

Back to Protocols


2 μl 10X Reaction Buffer
1 μl shrimp alkaline phosphatase
x μl DNA( 1 μg total concentration)
MilliQ up to 20 μl

Total Reaction of 20 μl


Incubate at 37 °C for 30 minutes
Heat inactivation at 65 °C for 15 minutes.