Revision as of 10:04, 14 August 2015

PCR with Taq DNA Polymerase (NEB):

Reaction Setup:

2.5 µl 10X Standard Taq Reaction buffer
2.5 µl dNTPS [2 mM]
0.5 µl Forward Primer [10 µM]
0.5 µl Reverse Primer [10 µM]
1 µl Template DNA
0.125 µl Taq DNA Polymerase
17.875 µl MilliQ Water

25 µl Total Reaction

PCR Program:

Step	Temperature	Time
Initial Denaturation	95 °C	30 seconds /5 Minutes for Colony PCR
35 Cycles	95 °C	30 seconds
	45 °C – 72 °C	60 seconds
	68 °C	1 minute / 1 kb
Final Extension	68 °C	5 minutes
Hold	4 °C	forever

@@ Line 44: / Line 44: @@
    </tr>
    <tr>
-     <td rowspan="3">35 Cycles</td>
+     <td rowspan="3"></br></br>35 Cycles</td>
      <td>95 °C</td>
      <td>30 seconds</td>