Difference between revisions of "Team:UiOslo Norway/Experiments/NMS medium"
| Line 18: | Line 18: | ||
43.8 mM MgSO4 * 6 H2O</br> | 43.8 mM MgSO4 * 6 H2O</br> | ||
9.0 mM CaCl2</br> | 9.0 mM CaCl2</br> | ||
| − | up to 1L Water </br> | + | up to 1L Water </br></br> |
</p> | </p> | ||
| Line 24: | Line 24: | ||
<b>Ethylenediaminetetraacetic acid ferric sodium salt</b></br> | <b>Ethylenediaminetetraacetic acid ferric sodium salt</b></br> | ||
103.5 mM FeEDTA</br> | 103.5 mM FeEDTA</br> | ||
| − | up to 100 ml Water</br> | + | up to 100 ml Water</br></br> |
</p> | </p> | ||
| Line 30: | Line 30: | ||
<b>Sodium Molybdate dihydrate</b></br> | <b>Sodium Molybdate dihydrate</b></br> | ||
1.07 mM NaMoO4 *2H2O</br> | 1.07 mM NaMoO4 *2H2O</br> | ||
| − | up to 500 ml Water</br> | + | up to 500 ml Water</br></br> |
</p> | </p> | ||
| Line 45: | Line 45: | ||
up to 500 ml Water</br> | up to 500 ml Water</br> | ||
</br> | </br> | ||
| − | Store dark. | + | Store dark.</br> |
</p> | </p> | ||
| Line 54: | Line 54: | ||
up to 100 ml Water</br> | up to 100 ml Water</br> | ||
</br> | </br> | ||
| − | Add H2O to 800 ml, adjust pH to 6,8 and add H2O to 1000 ml. Autoclave or sterile filter. | + | Add H2O to 800 ml, adjust pH to 6,8 and add H2O to 1000 ml. Autoclave or sterile filter.</br> |
</p> | </p> | ||
| Line 60: | Line 60: | ||
<b>Preparation of solutions for Trace elements solution Copper(II) sulfate pentahydrate</b></br> | <b>Preparation of solutions for Trace elements solution Copper(II) sulfate pentahydrate</b></br> | ||
400.5 mM CuSO4*5H2O</br> | 400.5 mM CuSO4*5H2O</br> | ||
| − | up to 100 ml H2O</br> | + | up to 100 ml H2O</br></br> |
</p> | </p> | ||
| Line 68: | Line 68: | ||
up to 1L Water</br> | up to 1L Water</br> | ||
</br> | </br> | ||
| − | Adjust to pH=3 after mixing to avoid precipitation. | + | Adjust to pH=3 after mixing to avoid precipitation.</br> |
</p> | </p> | ||
<p> | <p> | ||
| − | <b>Zinc sulfate heptahydrate<b></br> | + | <b>Zinc sulfate heptahydrate</b></br> |
347.75 mM ZnSO4*7H2O</br> | 347.75 mM ZnSO4*7H2O</br> | ||
| − | up to 100 ml Water</br> | + | up to 100 ml Water</br></br> |
</p> | </p> | ||
| Line 80: | Line 80: | ||
<b>Boric Acid</b></br> | <b>Boric Acid</b></br> | ||
161.73 mM H3BO3</br> | 161.73 mM H3BO3</br> | ||
| − | up to 100 ml Water</br> | + | up to 100 ml Water</br></br> |
</p> | </p> | ||
Revision as of 10:58, 14 August 2015
NMS medium
Back to Protocols10x NMS 98.9 mM KNO3 43.8 mM MgSO4 * 6 H2O 9.0 mM CaCl2 up to 1L Water
Ethylenediaminetetraacetic acid ferric sodium salt 103.5 mM FeEDTA up to 100 ml Water
Sodium Molybdate dihydrate 1.07 mM NaMoO4 *2H2O up to 500 ml Water
Trace Elements solution 0.8 mM CuSO4*5 H2O 1.8 mM FeSO4*7H2O 1.4 mM ZnSO4*7H2O 0.24 mM H3BO3 0.21 mM CoCl2*6H2O 0.74 mM EDTA-Na2 0.1 mM MnCL2*4H2O 42.07 µM NiCl2*6H2O up to 500 ml Water Store dark.
Phosphate buffer 198.25 mM Na2HPO4*12H2O 191.05 mM KH2PO4 up to 100 ml Water Add H2O to 800 ml, adjust pH to 6,8 and add H2O to 1000 ml. Autoclave or sterile filter.
Preparation of solutions for Trace elements solution Copper(II) sulfate pentahydrate 400.5 mM CuSO4*5H2O up to 100 ml H2O
Iron(II) sulfate heptahydrate 3.6 mM FeSO4*7H2O up to 1L Water Adjust to pH=3 after mixing to avoid precipitation.
Zinc sulfate heptahydrate 347.75 mM ZnSO4*7H2O up to 100 ml Water
Boric Acid 161.73 mM H3BO3 up to 100 ml Water