Difference between revisions of "Team:Bordeaux/Practices"
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<br>Three different approaches exist in synthetic biology: | <br>Three different approaches exist in synthetic biology: | ||
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<p align="justify" style="line-height: 105%"> - The metabolic engineering of the living beings by using <b>biobricks</b> (DNA sequences whose functions and assembly conditions are known). The biobricks are free of access on the WEB and can be synthesized on request. | <p align="justify" style="line-height: 105%"> - The metabolic engineering of the living beings by using <b>biobricks</b> (DNA sequences whose functions and assembly conditions are known). The biobricks are free of access on the WEB and can be synthesized on request. | ||
<p align="justify" style="line-height: 105%"> - The production of <b>minimal genomes</b> and <b>simplified organisms</b> where new functions can be added to realize a task. This approach is often used for the optimization of existing processes. | <p align="justify" style="line-height: 105%"> - The production of <b>minimal genomes</b> and <b>simplified organisms</b> where new functions can be added to realize a task. This approach is often used for the optimization of existing processes. | ||
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<p align="justify"> To avoid confusion about the terms "biosecurity", "biosafety" and "biohazard", some people gave a definition of each of these terms. </p> | <p align="justify"> To avoid confusion about the terms "biosecurity", "biosafety" and "biohazard", some people gave a definition of each of these terms. </p> | ||
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− | < | + | <p align="justify" style="line-height: 105%"> - Biosafety describes principles, technologies and containment practices put in place to avoid accidents and unintentional exposure to pathogens and toxins. |
− | < | + | <p align="justify" style="line-height: 105%"> - Biosecurity describes the protection measures put in place and management of important biological materials in laboratories, in order to prevent them from being accessed without authorization. |
− | < | + | <p align="justify" style="line-height: 105%"> - Biohazard includes, in this case, both biosafety and biosecurity. |
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<p> <b> Reference </b> : <a href =" http://www.ambafrance-uk.org/Biosecurite-dans-les-laboratoires"> http://www.ambafrance-uk.org/Biosecurite-dans-les-laboratoires </a> </p> | <p> <b> Reference </b> : <a href =" http://www.ambafrance-uk.org/Biosecurite-dans-les-laboratoires"> http://www.ambafrance-uk.org/Biosecurite-dans-les-laboratoires </a> </p> | ||
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<p align="justify"> The team who worked in the lab has received instructions of lab safety with safety training.</p> | <p align="justify"> The team who worked in the lab has received instructions of lab safety with safety training.</p> | ||
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− | < | + | <p align="justify" style="line-height: 105%"> - We learned about acting in case of an accident, where to find emergency showers, fire extinguishers and emergency doors. |
− | < | + | <p align="justify" style="line-height: 105%"> - Some requirements were discussed as wearing gloves and lab coat for most procedures. In some cases, during the use of ethidium bromide, nitrile gloves are mandatory. Other experiments must be conducted with safety glasses. It is also recommended to wear clothes offering a minimum of protection. |
− | < | + | <p align="justify" style="line-height: 105%"> - We also learned about working with chemicals products and how to dispose of them. Most products used are classified as non-hazardous (even for the sulfation protocol). Chemical products are just irritants and so handle it with gloves. In the lab, there is a specific solvent cabinet for liquid wastes. |
− | < | + | <p align="justify" style="line-height: 105%"> - Moreover, all biological waste will be autoclaved before disposal. All work is done on benches or under open-front hoods. |
− | < | + | <p align="justify" style="line-height: 105%"> - We worked with non-toxic organisms as <i> Escherichia coli </i>(BL21) and <i> Saccharomyces cerevisiae </i>(Invsc1) but, all waste that has been in contact with bacteria was put to bacterial waste in aseptic conditions. |
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Revision as of 13:52, 14 August 2015