Difference between revisions of "Team:UCLA/Notebook/Spider Silk Genetics/14 August 2015"

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=08/14/2015=
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==PCR amplify M1-12==
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Since previous PCRs using ICA elution as template did not work very well, first M1-12 PCR product was also tested as a template
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{| class="wikitable"
 +
|
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| ICA elution
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| PCR product
 +
|-
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| Q5 buff
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| 10 uL
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| 10 uL
 +
|-
 +
| dNTPs
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| 1 uL
 +
| 1 uL
 +
|-
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| F-03
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| 2.5 uL
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| 2.5 uL
 +
|-
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| G-03
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| 2.5 uL
 +
| 2.5 uL
 +
|-
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| ddH2O
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| 32.5 uL
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| 33.3 uL
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|-
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| Template
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| 1 uL
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| 0.2 uL
 +
|-
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| Q5 Polymerase
 +
| 0.5 uL
 +
| 0.5 uL
 +
|}

Revision as of 03:01, 15 August 2015

iGEM UCLA




08/14/2015

PCR amplify M1-12

Since previous PCRs using ICA elution as template did not work very well, first M1-12 PCR product was also tested as a template

ICA elution PCR product
Q5 buff 10 uL 10 uL
dNTPs 1 uL 1 uL
F-03 2.5 uL 2.5 uL
G-03 2.5 uL 2.5 uL
ddH2O 32.5 uL 33.3 uL
Template 1 uL 0.2 uL
Q5 Polymerase 0.5 uL 0.5 uL