Difference between revisions of "Team:UCLA/Notebook/Spider Silk Genetics/14 August 2015"

(→‎08/14/2015)
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| 0.5 uL
 
| 0.5 uL
 
| 0.5 uL
 
| 0.5 uL
 +
|}
 +
 +
{| class="wikitable"
 +
| 98C
 +
| 30 sec
 +
|-
 +
| 98C
 +
| 10 sec
 +
|-
 +
| 66C
 +
| 20 sec
 +
|-
 +
|
 +
| x23
 +
|-
 +
| 72C
 +
| 45 sec
 +
|-
 +
| 72C
 +
| 2 min
 +
|-
 +
| 12C
 +
| Hold
 
|}
 
|}

Revision as of 03:06, 15 August 2015

iGEM UCLA




08/14/2015

PCR amplify M1-12

Since previous PCRs using ICA elution as template did not work very well, first M1-12 PCR product was also tested as a template

ICA elution PCR product
Q5 buff 10 uL 10 uL
dNTPs 1 uL 1 uL
F-03 2.5 uL 2.5 uL
G-03 2.5 uL 2.5 uL
ddH2O 32.5 uL 33.3 uL
Template 1 uL 0.2 uL
Q5 Polymerase 0.5 uL 0.5 uL
98C 30 sec
98C 10 sec
66C 20 sec
x23
72C 45 sec
72C 2 min
12C Hold