Difference between revisions of "Team:UCLA/Notebook/Spider Silk Genetics/13 August 2015"

(Colony PCR for M1-9(T7) and M1SeqAB1)
(Colony PCR for M1-9(T7) and M1SeqAB1)
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Ran samples on 1.5%
 
Ran samples on 1.5%
  
[[File:2015-08-13_17hr_01min.tif |500px|thumb|left]]
+
[[File: 2015-08-13_17hr_01min.tif]]
  
 
==PCR amplification of M1-12==
 
==PCR amplification of M1-12==

Revision as of 22:05, 15 August 2015

iGEM UCLA




08/13/2015

Colony PCR for M1-9(T7) and M1SeqAB1

Same protocol from yesterday

Ran samples on 1.5%

File:2015-08-13 17hr 01min.tif

PCR amplification of M1-12

Q5 buffer 10 uL
dNTPs 1 uL
F-03 2.5 uL
G-03 2.5 uL
ddH2O 32.5 uL
Template 1 uL
Q5 0.5 uL


PCR0813.001.jpg