Difference between revisions of "Team:Technion Israel/Lab Notebook/Overview"

Line 160: Line 160:
 
                                         <p>Ordered the gBlock of 3α-HSD, which was optimized for E. coli codon usage.  We also ordered primers for the Gibson reaction for the gBlock.</p>
 
                                         <p>Ordered the gBlock of 3α-HSD, which was optimized for E. coli codon usage.  We also ordered primers for the Gibson reaction for the gBlock.</p>
 
                                         <p>Performed reverse PCR to the N155 plasmid and carried out a DPN1 reaction on the product.</p>
 
                                         <p>Performed reverse PCR to the N155 plasmid and carried out a DPN1 reaction on the product.</p>
−
                                        <p></p><p><i>Active team members this week: Adi and Alexey</i></p>
+
                                        <p><i>Active team members this week: Adi and Alexey</i></p>
  
 
</div>
 
</div>
Line 189: Line 189:
 
                                         <p>We performed PCR on the 3αHSD gBlock with only 10 cycles (as recommended), but received no band in the gel</p>
 
                                         <p>We performed PCR on the 3αHSD gBlock with only 10 cycles (as recommended), but received no band in the gel</p>
 
                                         <p>We performed PCR on the 3αHSD gBlock in higher temperatures, but still received no bands in the gel</p>
 
                                         <p>We performed PCR on the 3αHSD gBlock in higher temperatures, but still received no bands in the gel</p>
−
                                         <p></p><p><i>Active team members this week: Adi</i></p>
+
                                         <p><i>Active team members this week: Adi</i></p>
 
                                          
 
                                          
 
</div>
 
</div>
Line 216: Line 216:
 
<h2>E.coli</h2>
 
<h2>E.coli</h2>
 
<p>Performed PCR on the 3αHSD gBlock in lower temperatures, no bands in the gel.</p>
 
<p>Performed PCR on the 3αHSD gBlock in lower temperatures, no bands in the gel.</p>
−
                                        <p></p><p><i>Active team members this week: Alexey</i></p>
+
                                        <p><i>Active team members this week: Alexey</i></p>
  
 
</div>
 
</div>
Line 233: Line 233:
 
</div>
 
</div>
 
</div>
 
</div>
 +
<div class="week">
 +
<h1>Week 4: May 23-29</h1>
 +
<div class="week_content">
 +
<div class="bacillus">
 +
<h2>Bacillus</h2>
 +
<p>content</p>
 +
</div>
 +
<div class="ecoli">
 +
<h2>E.coli</h2>
 +
<p>Performed PCR on the 3αHSD gBlock in lower temperatures, no bands in the gel.</p>
 +
                                        <p><i>Active team members this week: Alexey</i></p>
 +
 +
</div>
 +
<div class="modeling">
 +
<h2>Modeling</h2>
 +
<p>content</p>
 +
</div>
 +
<div class="comb">
 +
<h2>Comb</h2>
 +
<p>content</p>
 +
</div>
 +
<div class="nadph">
 +
<h2>NADPH</h2>
 +
<p>content</p>
 +
</div>
 +
</div>
 +
</div>
 +
 +
<div class="week">
 +
<h1>Week 5: May 30-June 5</h1>
 +
<div class="week_content">
 +
<div class="bacillus">
 +
<h2>Bacillus</h2>
 +
<p>content</p>
 +
</div>
 +
<div class="ecoli">
 +
<h2>E.coli</h2>
 +
<p>Performed PCR on the 3αHSD gBlock in lower temperatures, no bands in the gel.</p>
 +
                                        <p><i>Active team members this week: Alexey</i></p>
 +
 +
</div>
 +
<div class="modeling">
 +
<h2>Modeling</h2>
 +
<p>content</p>
 +
</div>
 +
<div class="comb">
 +
<h2>Comb</h2>
 +
<p>content</p>
 +
</div>
 +
<div class="nadph">
 +
<h2>NADPH</h2>
 +
<p>content</p>
 +
</div>
 +
</div>
 +
</div>
 +
 +
<div class="week">
 +
<h1>Week 6: June 6-12</h1>
 +
<div class="week_content">
 +
<div class="bacillus">
 +
<h2>Bacillus</h2>
 +
<p>content</p>
 +
</div>
 +
<div class="ecoli">
 +
<h2>E.coli</h2>
 +
<p>Performed PCR on the 3αHSD gBlock in lower temperatures, no bands in the gel.</p>
 +
                                        <p><i>Active team members this week: Alexey</i></p>
 +
 +
</div>
 +
<div class="modeling">
 +
<h2>Modeling</h2>
 +
<p>content</p>
 +
</div>
 +
<div class="comb">
 +
<h2>Comb</h2>
 +
<p>content</p>
 +
</div>
 +
<div class="nadph">
 +
<h2>NADPH</h2>
 +
<p>content</p>
 +
</div>
 +
</div>
 +
</div>
 +
 +
<div class="week">
 +
<h1>Week 7: June 13-19</h1>
 +
<div class="week_content">
 +
<div class="bacillus">
 +
<h2>Bacillus</h2>
 +
<p>content</p>
 +
</div>
 +
<div class="ecoli">
 +
<h2>E.coli</h2>
 +
<p>Performed PCR on the 3αHSD gBlock in lower temperatures, no bands in the gel.</p>
 +
                                        <p><i>Active team members this week: Alexey</i></p>
 +
 +
</div>
 +
<div class="modeling">
 +
<h2>Modeling</h2>
 +
<p>content</p>
 +
</div>
 +
<div class="comb">
 +
<h2>Comb</h2>
 +
<p>content</p>
 +
</div>
 +
<div class="nadph">
 +
<h2>NADPH</h2>
 +
<p>content</p>
 +
</div>
 +
</div>
 +
</div>
 +
 +
<div class="week">
 +
<h1>Week 8: June 20-26</h1>
 +
<div class="week_content">
 +
<div class="bacillus">
 +
<h2>Bacillus</h2>
 +
<p>content</p>
 +
</div>
 +
<div class="ecoli">
 +
<h2>E.coli</h2>
 +
<p>Performed PCR on the 3αHSD gBlock in lower temperatures, no bands in the gel.</p>
 +
                                        <p><i>Active team members this week: Alexey</i></p>
 +
 +
</div>
 +
<div class="modeling">
 +
<h2>Modeling</h2>
 +
<p>content</p>
 +
</div>
 +
<div class="comb">
 +
<h2>Comb</h2>
 +
<p>content</p>
 +
</div>
 +
<div class="nadph">
 +
<h2>NADPH</h2>
 +
<p>content</p>
 +
</div>
 +
</div>
 +
</div>
 +
<div class="week">
 +
<h1>Week 9: June 27-July 3</h1>
 +
<div class="week_content">
 +
<div class="bacillus">
 +
<h2>Bacillus</h2>
 +
<p>content</p>
 +
</div>
 +
<div class="ecoli">
 +
<h2>E.coli</h2>
 +
<p>Performed PCR on the 3αHSD gBlock in lower temperatures, no bands in the gel.</p>
 +
                                        <p><i>Active team members this week: Alexey</i></p>
 +
 +
</div>
 +
<div class="modeling">
 +
<h2>Modeling</h2>
 +
<p>content</p>
 +
</div>
 +
<div class="comb">
 +
<h2>Comb</h2>
 +
<p>content</p>
 +
</div>
 +
<div class="nadph">
 +
<h2>NADPH</h2>
 +
<p>content</p>
 +
</div>
 +
</div>
 +
</div>
 +
<div class="week">
 +
<h1>Week 10: July 4-10</h1>
 +
<div class="week_content">
 +
<div class="bacillus">
 +
<h2>Bacillus</h2>
 +
<p>content</p>
 +
</div>
 +
<div class="ecoli">
 +
<h2>E.coli</h2>
 +
<p>We took a break for our exams.</p>
 +
                                       
 +
</div>
 +
<div class="modeling">
 +
<h2>Modeling</h2>
 +
<p>content</p>
 +
</div>
 +
<div class="comb">
 +
<h2>Comb</h2>
 +
<p>content</p>
 +
</div>
 +
<div class="nadph">
 +
<h2>NADPH</h2>
 +
<p>content</p>
 +
</div>
 +
</div>
 +
</div>
 +
<div class="week">
 +
<h1>Week 11: July 11-17</h1>
 +
<div class="week_content">
 +
<div class="bacillus">
 +
<h2>Bacillus</h2>
 +
<p>content</p>
 +
</div>
 +
<div class="ecoli">
 +
<h2>E.coli</h2>
 +
<p>Performed PCR on the 3αHSD gBlock in lower temperatures, no bands in the gel.</p>
 +
                                        <p><i>Active team members this week: Alexey</i></p>
 +
 +
</div>
 +
<div class="modeling">
 +
<h2>Modeling</h2>
 +
<p>content</p>
 +
</div>
 +
<div class="comb">
 +
<h2>Comb</h2>
 +
<p>content</p>
 +
</div>
 +
<div class="nadph">
 +
<h2>NADPH</h2>
 +
<p>content</p>
 +
</div>
 +
</div>
 +
</div>
 +
<div class="week">
 +
<h1>Week 12: July 18-24</h1>
 +
<div class="week_content">
 +
<div class="bacillus">
 +
<h2>Bacillus</h2>
 +
<p>content</p>
 +
</div>
 +
<div class="ecoli">
 +
<h2>E.coli</h2>
 +
<p>Performed PCR on the 3αHSD gBlock in lower temperatures, no bands in the gel.</p>
 +
                                        <p><i>Active team members this week: Alexey</i></p>
 +
 +
</div>
 +
<div class="modeling">
 +
<h2>Modeling</h2>
 +
<p>content</p>
 +
</div>
 +
<div class="comb">
 +
<h2>Comb</h2>
 +
<p>content</p>
 +
</div>
 +
<div class="nadph">
 +
<h2>NADPH</h2>
 +
<p>content</p>
 +
</div>
 +
</div>
 +
</div>
 +
<div class="week">
 +
<h1>Week 13: July 25-31</h1>
 +
<div class="week_content">
 +
<div class="bacillus">
 +
<h2>Bacillus</h2>
 +
<p>content</p>
 +
</div>
 +
<div class="ecoli">
 +
<h2>E.coli</h2>
 +
<p>Performed PCR on the 3αHSD gBlock in lower temperatures, no bands in the gel.</p>
 +
                                        <p><i>Active team members this week: Alexey</i></p>
 +
 +
</div>
 +
<div class="modeling">
 +
<h2>Modeling</h2>
 +
<p>content</p>
 +
</div>
 +
<div class="comb">
 +
<h2>Comb</h2>
 +
<p>content</p>
 +
</div>
 +
<div class="nadph">
 +
<h2>NADPH</h2>
 +
<p>content</p>
 +
</div>
 +
</div>
 +
</div>
 +
<div class="week">
 +
<h1>Week 14: August 1-7</h1>
 +
<div class="week_content">
 +
<div class="bacillus">
 +
<h2>Bacillus</h2>
 +
<p>content</p>
 +
</div>
 +
<div class="ecoli">
 +
<h2>E.coli</h2>
 +
<p>Performed PCR on the 3αHSD gBlock in lower temperatures, no bands in the gel.</p>
 +
                                        <p><i>Active team members this week: Alexey</i></p>
 +
 +
</div>
 +
<div class="modeling">
 +
<h2>Modeling</h2>
 +
<p>content</p>
 +
</div>
 +
<div class="comb">
 +
<h2>Comb</h2>
 +
<p>content</p>
 +
</div>
 +
<div class="nadph">
 +
<h2>NADPH</h2>
 +
<p>content</p>
 +
</div>
 +
</div>
 +
</div>
 +
<div class="week">
 +
<h1>Week 15: August 8-14</h1>
 +
<div class="week_content">
 +
<div class="bacillus">
 +
<h2>Bacillus</h2>
 +
<p>content</p>
 +
</div>
 +
<div class="ecoli">
 +
<h2>E.coli</h2>
 +
<p>Performed PCR on the 3αHSD gBlock in lower temperatures, no bands in the gel.</p>
 +
                                        <p><i>Active team members this week: Alexey</i></p>
 +
 +
</div>
 +
<div class="modeling">
 +
<h2>Modeling</h2>
 +
<p>content</p>
 +
</div>
 +
<div class="comb">
 +
<h2>Comb</h2>
 +
<p>content</p>
 +
</div>
 +
<div class="nadph">
 +
<h2>NADPH</h2>
 +
<p>content</p>
 +
</div>
 +
</div>
 +
</div>
 +
<div class="week">
 +
<h1>Week 16: August 15-21</h1>
 +
<div class="week_content">
 +
<div class="bacillus">
 +
<h2>Bacillus</h2>
 +
<p>content</p>
 +
</div>
 +
<div class="ecoli">
 +
<h2>E.coli</h2>
 +
<p>Performed PCR on the 3αHSD gBlock in lower temperatures, no bands in the gel.</p>
 +
                                        <p><i>Active team members this week: Alexey</i></p>
 +
 +
</div>
 +
<div class="modeling">
 +
<h2>Modeling</h2>
 +
<p>content</p>
 +
</div>
 +
<div class="comb">
 +
<h2>Comb</h2>
 +
<p>content</p>
 +
</div>
 +
<div class="nadph">
 +
<h2>NADPH</h2>
 +
<p>content</p>
 +
</div>
 +
</div>
 +
</div>
 +
<div class="week">
 +
<h1>Week 17: August 22-28</h1>
 +
<div class="week_content">
 +
<div class="bacillus">
 +
<h2>Bacillus</h2>
 +
<p>content</p>
 +
</div>
 +
<div class="ecoli">
 +
<h2>E.coli</h2>
 +
<p>Performed PCR on the 3αHSD gBlock in lower temperatures, no bands in the gel.</p>
 +
                                        <p><i>Active team members this week: Alexey</i></p>
 +
 +
</div>
 +
<div class="modeling">
 +
<h2>Modeling</h2>
 +
<p>content</p>
 +
</div>
 +
<div class="comb">
 +
<h2>Comb</h2>
 +
<p>content</p>
 +
</div>
 +
<div class="nadph">
 +
<h2>NADPH</h2>
 +
<p>content</p>
 +
</div>
 +
</div>
 +
</div>
 +
<div class="week">
 +
<h1>Week 18: August 29-September 4</h1>
 +
<div class="week_content">
 +
<div class="bacillus">
 +
<h2>Bacillus</h2>
 +
<p>content</p>
 +
</div>
 +
<div class="ecoli">
 +
<h2>E.coli</h2>
 +
<p>Performed PCR on the 3αHSD gBlock in lower temperatures, no bands in the gel.</p>
 +
                                        <p><i>Active team members this week: Alexey</i></p>
 +
 +
</div>
 +
<div class="modeling">
 +
<h2>Modeling</h2>
 +
<p>content</p>
 +
</div>
 +
<div class="comb">
 +
<h2>Comb</h2>
 +
<p>content</p>
 +
</div>
 +
<div class="nadph">
 +
<h2>NADPH</h2>
 +
<p>content</p>
 +
</div>
 +
</div>
 +
</div>
 +
<div class="week">
 +
<h1>Week 19: September 5-11</h1>
 +
<div class="week_content">
 +
<div class="bacillus">
 +
<h2>Bacillus</h2>
 +
<p>content</p>
 +
</div>
 +
<div class="ecoli">
 +
<h2>E.coli</h2>
 +
<p>Performed PCR on the 3αHSD gBlock in lower temperatures, no bands in the gel.</p>
 +
                                        <p><i>Active team members this week: Alexey</i></p>
 +
 +
</div>
 +
<div class="modeling">
 +
<h2>Modeling</h2>
 +
<p>content</p>
 +
</div>
 +
<div class="comb">
 +
<h2>Comb</h2>
 +
<p>content</p>
 +
</div>
 +
<div class="nadph">
 +
<h2>NADPH</h2>
 +
<p>content</p>
 +
</div>
 +
</div>
 +
</div>
 +
<div class="week">
 +
<h1>Week 20: September 12-18</h1>
 +
<div class="week_content">
 +
<div class="bacillus">
 +
<h2>Bacillus</h2>
 +
<p>content</p>
 +
</div>
 +
<div class="ecoli">
 +
<h2>E.coli</h2>
 +
<p>Performed PCR on the 3αHSD gBlock in lower temperatures, no bands in the gel.</p>
 +
                                        <p><i>Active team members this week: Alexey</i></p>
 +
 +
</div>
 +
<div class="modeling">
 +
<h2>Modeling</h2>
 +
<p>content</p>
 +
</div>
 +
<div class="comb">
 +
<h2>Comb</h2>
 +
<p>content</p>
 +
</div>
 +
<div class="nadph">
 +
<h2>NADPH</h2>
 +
<p>content</p>
 +
</div>
 +
</div>
 +
</div>
 +
 
</div>
 
</div>
 
</div>
 
</div>

Revision as of 07:59, 16 August 2015

Team: Technion 2015

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Lab Notebook Overview

Week 1: May 2-8

Bacillus

content

E.coli

Planned the Gibson reaction for our gBlock of 3α-HSD and the plasmid N155 (from our mentors) containing pT7, RBS and mcherry.

Ordered the gBlock of 3α-HSD, which was optimized for E. coli codon usage. We also ordered primers for the Gibson reaction for the gBlock.

Performed reverse PCR to the N155 plasmid and carried out a DPN1 reaction on the product.

Active team members this week: Adi and Alexey

Modeling

content

Comb

content

NADPH

content

Week 2: May 9-15

Bacillus

content

E.coli

Our gBlock arrived!!!

We performed PCR on the 3αHSD gBlock with only 10 cycles (as recommended), but received no band in the gel

We performed PCR on the 3αHSD gBlock in higher temperatures, but still received no bands in the gel

Active team members this week: Adi

Modeling

content

Comb

content

NADPH

content

Week 3: May 16-22

Bacillus

content

E.coli

Performed PCR on the 3αHSD gBlock in lower temperatures, no bands in the gel.

Active team members this week: Alexey

Modeling

content

Comb

content

NADPH

content

Week 4: May 23-29

Bacillus

content

E.coli

Performed PCR on the 3αHSD gBlock in lower temperatures, no bands in the gel.

Active team members this week: Alexey

Modeling

content

Comb

content

NADPH

content

Week 5: May 30-June 5

Bacillus

content

E.coli

Performed PCR on the 3αHSD gBlock in lower temperatures, no bands in the gel.

Active team members this week: Alexey

Modeling

content

Comb

content

NADPH

content

Week 6: June 6-12

Bacillus

content

E.coli

Performed PCR on the 3αHSD gBlock in lower temperatures, no bands in the gel.

Active team members this week: Alexey

Modeling

content

Comb

content

NADPH

content

Week 7: June 13-19

Bacillus

content

E.coli

Performed PCR on the 3αHSD gBlock in lower temperatures, no bands in the gel.

Active team members this week: Alexey

Modeling

content

Comb

content

NADPH

content

Week 8: June 20-26

Bacillus

content

E.coli

Performed PCR on the 3αHSD gBlock in lower temperatures, no bands in the gel.

Active team members this week: Alexey

Modeling

content

Comb

content

NADPH

content

Week 9: June 27-July 3

Bacillus

content

E.coli

Performed PCR on the 3αHSD gBlock in lower temperatures, no bands in the gel.

Active team members this week: Alexey

Modeling

content

Comb

content

NADPH

content

Week 10: July 4-10

Bacillus

content

E.coli

We took a break for our exams.

Modeling

content

Comb

content

NADPH

content

Week 11: July 11-17

Bacillus

content

E.coli

Performed PCR on the 3αHSD gBlock in lower temperatures, no bands in the gel.

Active team members this week: Alexey

Modeling

content

Comb

content

NADPH

content

Week 12: July 18-24

Bacillus

content

E.coli

Performed PCR on the 3αHSD gBlock in lower temperatures, no bands in the gel.

Active team members this week: Alexey

Modeling

content

Comb

content

NADPH

content

Week 13: July 25-31

Bacillus

content

E.coli

Performed PCR on the 3αHSD gBlock in lower temperatures, no bands in the gel.

Active team members this week: Alexey

Modeling

content

Comb

content

NADPH

content

Week 14: August 1-7

Bacillus

content

E.coli

Performed PCR on the 3αHSD gBlock in lower temperatures, no bands in the gel.

Active team members this week: Alexey

Modeling

content

Comb

content

NADPH

content

Week 15: August 8-14

Bacillus

content

E.coli

Performed PCR on the 3αHSD gBlock in lower temperatures, no bands in the gel.

Active team members this week: Alexey

Modeling

content

Comb

content

NADPH

content

Week 16: August 15-21

Bacillus

content

E.coli

Performed PCR on the 3αHSD gBlock in lower temperatures, no bands in the gel.

Active team members this week: Alexey

Modeling

content

Comb

content

NADPH

content

Week 17: August 22-28

Bacillus

content

E.coli

Performed PCR on the 3αHSD gBlock in lower temperatures, no bands in the gel.

Active team members this week: Alexey

Modeling

content

Comb

content

NADPH

content

Week 18: August 29-September 4

Bacillus

content

E.coli

Performed PCR on the 3αHSD gBlock in lower temperatures, no bands in the gel.

Active team members this week: Alexey

Modeling

content

Comb

content

NADPH

content

Week 19: September 5-11

Bacillus

content

E.coli

Performed PCR on the 3αHSD gBlock in lower temperatures, no bands in the gel.

Active team members this week: Alexey

Modeling

content

Comb

content

NADPH

content

Week 20: September 12-18

Bacillus

content

E.coli

Performed PCR on the 3αHSD gBlock in lower temperatures, no bands in the gel.

Active team members this week: Alexey

Modeling

content

Comb

content

NADPH

content

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