Difference between revisions of "Team:NAIT Edmonton/Protocols"

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     <center><h1>Run PCR</h1></center>
 
     <center><h1>Run PCR</h1></center>
 +
    <p><b>Note:</b>A PCR gradient was conducted before running a PCR of all our sequences to determine the optimal annealing temperature needed for best results.</p>
 
     <p>1. Perform PCR with the following Cycle Protocol</p><br>
 
     <p>1. Perform PCR with the following Cycle Protocol</p><br>
  

Revision as of 22:07, 17 August 2015

Team NAIT 2015

Experimental Design

Go through our interactive experimental design flow chart! Many of our protocols are manufacturer specicfied; however, some are customized by us! PDFs of protocols can also be found

Theorizing our Sequences
Literature has shown certain proteins inherently stain in colour.
Looked up characteristics of said proteins
Isolated and identified the unique characteristics of said proteins so that we can manually write our own sequences and generate custom proteins.
Writing our Sequences
Went down to base pair level and wrote out our sequences with the defining characteristics identified in literature.
PCR
Digestion and Ligation
Transforming Bacteria
Validating the Transformation
Protein Isolation and Purification
SDS PAGE and Silver Staining