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Revision as of 10:09, 19 August 2015
Contents
About The Notes
Our notes are the abbreviations of the experimental process, including titles, genes we transformed, plasmids we used, and resistances on the plasmids. We categorized these notes by date. Moreover, team members followed the iGEM protocol in each experimental step in order to get better experimental efficiency and the results we had expected.
Material and Methods
Wet Lab Protocol
- Wet Lab ProtocolEnglish version download
- 實驗手冊中文版本下載
Insect Experiment Protocol
- Insect Experiment ProtocolEnglish version download
Note
- April
2015 - May
2015 - June
2015 - July
2015 - August
2015 - September
2015
April 2015
- Sun.
- Mon.
- Tue.
- Wed.
- Thu.
- Fri.
- Sat.
- 1
- 2
- 3
- 4
- 5
- 6
- 7
- 8
- 9
- 10
- 11
- 12
- 13
- 14
- 15
- 16
- 17
- 18
- 19
- 20
- 21
- 22
- 23
- 24
- 25
- 26
- 27
- 28
- 29
- 30
4/11
Transformation of [PSB1K3] Kr, [PSB1A3] Ar, [PSB1C3] Cr (G2)
Transformation of [Plac+PSB1A3] Ar, [mGFP+PSB1A3] Ar (G3)
Transformation of [PSB1K3] Kr,[PSB1A3] Ar, [PSB1C3] Cr (G1)
4/12
Cultivation of [PSB1K3] Kr, [PSB1C3] Cr (G2)
Cultivation of [PSB1A3] Ar, [PSB1C3] Cr (G1)
Cultivation of [mGFP] Ar (G3)
4/13
Mini-prep of [mGFP] Ar, [PSB1K3] Kr (G3)
Mini-prep of [PSB1K3] Kr, [PSB1C3] Cr (G2)
Mini-prep of [PSB1A3] Ar, [PSB1C3] Cr (G1)
4/17
Transformation of [PSB1K3] Kr (G1)
4/18
Cultivation of [PSB1K3] Kr (G1)
4/19
Transformation of [Plac+PSB1A3] Ar, [PSB1K3] Kr, [PSB1A3] Ar, [PSB1C3] Cr (G3)
Mini-prep of [PSB1K3] Kr (G1)
4/20
Transformation of [PSB1A3] Ar (G2)
Electrophoresis of [PSB1K3] Kr, [PSB1C3] Cr plasmid (G2)
4/21
Cultivation of [PSB1K3] Kr, [PSB1A3] Ar, [PSB1C3] Cr (G2)
4/22
Mini-prep of [PSB1K3] Kr, [PSB1A3] Ar, [PSB1C3] Cr (G2)
4/28
Electrophoresis of digested [PSB1K3] Kr(EP), [PSB1C3] Cr(EP),[PSB1A3] Ar(EP) (G1)
Cultivation of [PSB1C3] Cr (G1)
4/29
Mini-prep of [PSB1C3] Cr (G1)
Mini-prep of [PSB1C3] Cr (G2)
Transformation of [B0032] Ar, and [BFP] Cr (G2)
Cultivation of [PSB1K3] Kr, [PSB1A3] Ar (G1)
Cultivation of [B0032] Ar, and [BFP] Cr (G2)
4/30
Mini-prep of [PSB1A3] Ar (G1)
Mini-prep of [B0032] Ar, and [BFP] Cr (G2)
Transformation of [B0032] Ar, and [BFP] Cr, [T7promoter] Ar Kr and [T7 polymerase] Cr (G2)
Transformation of [J23101] Ar,[K592100] Cr,[B0032] Ar,[B0015] Ar (G1)
Digestion of [PSBIC3] Cr(EP), [B0032] Ar(ES), [BFP] Cr(XP) mini (G2)
Electrophoresis of plasmid and digested prouct of[PSBIC3] Cr(EP), [B0032] Ar(ES), [BFP] Cr(XP) mini (G2)
May 2015
- Sun.
- Mon.
- Tue.
- Wed.
- Thu.
- Fri.
- Sat.
- 1
- 2
- 3
- 4
- 5
- 6
- 7
- 8
- 9
- 10
- 11
- 12
- 13
- 14
- 15
- 16
- 17
- 18
- 19
- 20
- 21
- 22
- 23
- 24
- 25
- 26
- 27
- 28
- 29
- 30
- 31
4/11
Transformation of [PSB1K3] Kr, [PSB1A3] Ar, [PSB1C3] Cr (G2)
Transformation of [Plac+PSB1A3] Ar, [mGFP+PSB1A3] Ar (G3)
Transformation of [PSB1K3] Kr,[PSB1A3] Ar, [PSB1C3] Cr (G1)
4/12
Cultivation of [PSB1K3] Kr, [PSB1C3] Cr (G2)
Cultivation of [PSB1A3] Ar, [PSB1C3] Cr (G1)
Cultivation of [mGFP] Ar (G3)
4/13
Mini-prep of [mGFP] Ar, [PSB1K3] Kr (G3)
Mini-prep of [PSB1K3] Kr, [PSB1C3] Cr (G2)
Mini-prep of [PSB1A3] Ar, [PSB1C3] Cr (G1)
4/17
Transformation of [PSB1K3] Kr (G1)
4/18
Cultivation of [PSB1K3] Kr (G1)
4/19
Transformation of [Plac+PSB1A3] Ar, [PSB1K3] Kr, [PSB1A3] Ar, [PSB1C3] Cr (G3)
Mini-prep of [PSB1K3] Kr (G1)
4/20
Transformation of [PSB1A3] Ar (G2)
Electrophoresis of [PSB1K3] Kr, [PSB1C3] Cr plasmid (G2)
4/21
Cultivation of [PSB1K3] Kr, [PSB1A3] Ar, [PSB1C3] Cr (G2)
4/22
Mini-prep of [PSB1K3] Kr, [PSB1A3] Ar, [PSB1C3] Cr (G2)
4/28
Electrophoresis of digested [PSB1K3] Kr(EP), [PSB1C3] Cr(EP),[PSB1A3] Ar(EP) (G1)
Cultivation of [PSB1C3] Cr (G1)
4/29
Mini-prep of [PSB1C3] Cr (G1)
Mini-prep of [PSB1C3] Cr (G2)
Transformation of [B0032] Ar, and [BFP] Cr (G2)
Cultivation of [PSB1K3] Kr, [PSB1A3] Ar (G1)
Cultivation of [B0032] Ar, and [BFP] Cr (G2)
4/30
Mini-prep of [PSB1A3] Ar (G1)
Mini-prep of [B0032] Ar, and [BFP] Cr (G2)
Transformation of [B0032] Ar, and [BFP] Cr, [T7promoter] Ar Kr and [T7 polymerase] Cr (G2)
Transformation of [J23101] Ar,[K592100] Cr,[B0032] Ar,[B0015] Ar (G1)
Digestion of [PSBIC3] Cr(EP), [B0032] Ar(ES), [BFP] Cr(XP) mini (G2)
Electrophoresis of plasmid and digested prouct of[PSBIC3] Cr(EP), [B0032] Ar(ES), [BFP] Cr(XP) mini (G2)
June 2015
- Sun.
- Mon.
- Tue.
- Wed.
- Thu.
- Fri.
- Sat.
- 1
- 2
- 3
- 4
- 5
- 6
- 7
- 8
- 9
- 10
- 11
- 12
- 13
- 14
- 15
- 16
- 17
- 18
- 19
- 20
- 21
- 22
- 23
- 24
- 25
- 26
- 27
- 28
- 29
- 30
4/11
Transformation of [PSB1K3] Kr, [PSB1A3] Ar, [PSB1C3] Cr (G2)
Transformation of [Plac+PSB1A3] Ar, [mGFP+PSB1A3] Ar (G3)
Transformation of [PSB1K3] Kr,[PSB1A3] Ar, [PSB1C3] Cr (G1)
4/12
Cultivation of [PSB1K3] Kr, [PSB1C3] Cr (G2)
Cultivation of [PSB1A3] Ar, [PSB1C3] Cr (G1)
Cultivation of [mGFP] Ar (G3)
4/13
Mini-prep of [mGFP] Ar, [PSB1K3] Kr (G3)
Mini-prep of [PSB1K3] Kr, [PSB1C3] Cr (G2)
Mini-prep of [PSB1A3] Ar, [PSB1C3] Cr (G1)
4/17
Transformation of [PSB1K3] Kr (G1)
4/18
Cultivation of [PSB1K3] Kr (G1)
4/19
Transformation of [Plac+PSB1A3] Ar, [PSB1K3] Kr, [PSB1A3] Ar, [PSB1C3] Cr (G3)
Mini-prep of [PSB1K3] Kr (G1)
4/20
Transformation of [PSB1A3] Ar (G2)
Electrophoresis of [PSB1K3] Kr, [PSB1C3] Cr plasmid (G2)
4/21
Cultivation of [PSB1K3] Kr, [PSB1A3] Ar, [PSB1C3] Cr (G2)
4/22
Mini-prep of [PSB1K3] Kr, [PSB1A3] Ar, [PSB1C3] Cr (G2)
4/28
Electrophoresis of digested [PSB1K3] Kr(EP), [PSB1C3] Cr(EP),[PSB1A3] Ar(EP) (G1)
Cultivation of [PSB1C3] Cr (G1)
4/29
Mini-prep of [PSB1C3] Cr (G1)
Mini-prep of [PSB1C3] Cr (G2)
Transformation of [B0032] Ar, and [BFP] Cr (G2)
Cultivation of [PSB1K3] Kr, [PSB1A3] Ar (G1)
Cultivation of [B0032] Ar, and [BFP] Cr (G2)
4/30
Mini-prep of [PSB1A3] Ar (G1)
Mini-prep of [B0032] Ar, and [BFP] Cr (G2)
Transformation of [B0032] Ar, and [BFP] Cr, [T7promoter] Ar Kr and [T7 polymerase] Cr (G2)
Transformation of [J23101] Ar,[K592100] Cr,[B0032] Ar,[B0015] Ar (G1)
Digestion of [PSBIC3] Cr(EP), [B0032] Ar(ES), [BFP] Cr(XP) mini (G2)
Electrophoresis of plasmid and digested prouct of[PSBIC3] Cr(EP), [B0032] Ar(ES), [BFP] Cr(XP) mini (G2)
July 2015
- Sun.
- Mon.
- Tue.
- Wed.
- Thu.
- Fri.
- Sat.
- 1
- 2
- 3
- 4
- 5
- 6
- 7
- 8
- 9
- 10
- 11
- 12
- 13
- 14
- 15
- 16
- 17
- 18
- 19
- 20
- 21
- 22
- 23
- 24
- 25
- 26
- 27
- 28
- 29
- 30
- 31
4/11
Transformation of [PSB1K3] Kr, [PSB1A3] Ar, [PSB1C3] Cr (G2)
Transformation of [Plac+PSB1A3] Ar, [mGFP+PSB1A3] Ar (G3)
Transformation of [PSB1K3] Kr,[PSB1A3] Ar, [PSB1C3] Cr (G1)
4/12
Cultivation of [PSB1K3] Kr, [PSB1C3] Cr (G2)
Cultivation of [PSB1A3] Ar, [PSB1C3] Cr (G1)
Cultivation of [mGFP] Ar (G3)
4/13
Mini-prep of [mGFP] Ar, [PSB1K3] Kr (G3)
Mini-prep of [PSB1K3] Kr, [PSB1C3] Cr (G2)
Mini-prep of [PSB1A3] Ar, [PSB1C3] Cr (G1)
4/17
Transformation of [PSB1K3] Kr (G1)
4/18
Cultivation of [PSB1K3] Kr (G1)
4/19
Transformation of [Plac+PSB1A3] Ar, [PSB1K3] Kr, [PSB1A3] Ar, [PSB1C3] Cr (G3)
Mini-prep of [PSB1K3] Kr (G1)
4/20
Transformation of [PSB1A3] Ar (G2)
Electrophoresis of [PSB1K3] Kr, [PSB1C3] Cr plasmid (G2)
4/21
Cultivation of [PSB1K3] Kr, [PSB1A3] Ar, [PSB1C3] Cr (G2)
4/22
Mini-prep of [PSB1K3] Kr, [PSB1A3] Ar, [PSB1C3] Cr (G2)
4/28
Electrophoresis of digested [PSB1K3] Kr(EP), [PSB1C3] Cr(EP),[PSB1A3] Ar(EP) (G1)
Cultivation of [PSB1C3] Cr (G1)
4/29
Mini-prep of [PSB1C3] Cr (G1)
Mini-prep of [PSB1C3] Cr (G2)
Transformation of [B0032] Ar, and [BFP] Cr (G2)
Cultivation of [PSB1K3] Kr, [PSB1A3] Ar (G1)
Cultivation of [B0032] Ar, and [BFP] Cr (G2)
4/30
Mini-prep of [PSB1A3] Ar (G1)
Mini-prep of [B0032] Ar, and [BFP] Cr (G2)
Transformation of [B0032] Ar, and [BFP] Cr, [T7promoter] Ar Kr and [T7 polymerase] Cr (G2)
Transformation of [J23101] Ar,[K592100] Cr,[B0032] Ar,[B0015] Ar (G1)
Digestion of [PSBIC3] Cr(EP), [B0032] Ar(ES), [BFP] Cr(XP) mini (G2)
Electrophoresis of plasmid and digested prouct of[PSBIC3] Cr(EP), [B0032] Ar(ES), [BFP] Cr(XP) mini (G2)
August 2015
- Sun.
- Mon.
- Tue.
- Wed.
- Thu.
- Fri.
- Sat.
- 1
- 2
- 3
- 4
- 5
- 6
- 7
- 8
- 9
- 10
- 11
- 12
- 13
- 14
- 15
- 16
- 17
- 18
- 19
- 20
- 21
- 22
- 23
- 24
- 25
- 26
- 27
- 28
- 29
- 30
- 31
4/11
Transformation of [PSB1K3] Kr, [PSB1A3] Ar, [PSB1C3] Cr (G2)
Transformation of [Plac+PSB1A3] Ar, [mGFP+PSB1A3] Ar (G3)
Transformation of [PSB1K3] Kr,[PSB1A3] Ar, [PSB1C3] Cr (G1)
4/12
Cultivation of [PSB1K3] Kr, [PSB1C3] Cr (G2)
Cultivation of [PSB1A3] Ar, [PSB1C3] Cr (G1)
Cultivation of [mGFP] Ar (G3)
4/13
Mini-prep of [mGFP] Ar, [PSB1K3] Kr (G3)
Mini-prep of [PSB1K3] Kr, [PSB1C3] Cr (G2)
Mini-prep of [PSB1A3] Ar, [PSB1C3] Cr (G1)
4/17
Transformation of [PSB1K3] Kr (G1)
4/18
Cultivation of [PSB1K3] Kr (G1)
4/19
Transformation of [Plac+PSB1A3] Ar, [PSB1K3] Kr, [PSB1A3] Ar, [PSB1C3] Cr (G3)
Mini-prep of [PSB1K3] Kr (G1)
4/20
Transformation of [PSB1A3] Ar (G2)
Electrophoresis of [PSB1K3] Kr, [PSB1C3] Cr plasmid (G2)
4/21
Cultivation of [PSB1K3] Kr, [PSB1A3] Ar, [PSB1C3] Cr (G2)
4/22
Mini-prep of [PSB1K3] Kr, [PSB1A3] Ar, [PSB1C3] Cr (G2)
4/28
Electrophoresis of digested [PSB1K3] Kr(EP), [PSB1C3] Cr(EP),[PSB1A3] Ar(EP) (G1)
Cultivation of [PSB1C3] Cr (G1)
4/29
Mini-prep of [PSB1C3] Cr (G1)
Mini-prep of [PSB1C3] Cr (G2)
Transformation of [B0032] Ar, and [BFP] Cr (G2)
Cultivation of [PSB1K3] Kr, [PSB1A3] Ar (G1)
Cultivation of [B0032] Ar, and [BFP] Cr (G2)
4/30
Mini-prep of [PSB1A3] Ar (G1)
Mini-prep of [B0032] Ar, and [BFP] Cr (G2)
Transformation of [B0032] Ar, and [BFP] Cr, [T7promoter] Ar Kr and [T7 polymerase] Cr (G2)
Transformation of [J23101] Ar,[K592100] Cr,[B0032] Ar,[B0015] Ar (G1)
Digestion of [PSBIC3] Cr(EP), [B0032] Ar(ES), [BFP] Cr(XP) mini (G2)
Electrophoresis of plasmid and digested prouct of[PSBIC3] Cr(EP), [B0032] Ar(ES), [BFP] Cr(XP) mini (G2)
September 2015
- Sun.
- Mon.
- Tue.
- Wed.
- Thu.
- Fri.
- Sat.
- 1
- 2
- 3
- 4
- 5
- 6
- 7
- 8
- 9
- 10
- 11
- 12
- 13
- 14
- 15
- 16
- 17
- 18
- 19
- 20
- 21
- 22
- 23
- 24
- 25
- 26
- 27
- 28
- 29
- 30
4/11
Transformation of [PSB1K3] Kr, [PSB1A3] Ar, [PSB1C3] Cr (G2)
Transformation of [Plac+PSB1A3] Ar, [mGFP+PSB1A3] Ar (G3)
Transformation of [PSB1K3] Kr,[PSB1A3] Ar, [PSB1C3] Cr (G1)
4/12
Cultivation of [PSB1K3] Kr, [PSB1C3] Cr (G2)
Cultivation of [PSB1A3] Ar, [PSB1C3] Cr (G1)
Cultivation of [mGFP] Ar (G3)
4/13
Mini-prep of [mGFP] Ar, [PSB1K3] Kr (G3)
Mini-prep of [PSB1K3] Kr, [PSB1C3] Cr (G2)
Mini-prep of [PSB1A3] Ar, [PSB1C3] Cr (G1)
4/17
Transformation of [PSB1K3] Kr (G1)
4/18
Cultivation of [PSB1K3] Kr (G1)
4/19
Transformation of [Plac+PSB1A3] Ar, [PSB1K3] Kr, [PSB1A3] Ar, [PSB1C3] Cr (G3)
Mini-prep of [PSB1K3] Kr (G1)
4/20
Transformation of [PSB1A3] Ar (G2)
Electrophoresis of [PSB1K3] Kr, [PSB1C3] Cr plasmid (G2)
4/21
Cultivation of [PSB1K3] Kr, [PSB1A3] Ar, [PSB1C3] Cr (G2)
4/22
Mini-prep of [PSB1K3] Kr, [PSB1A3] Ar, [PSB1C3] Cr (G2)
4/28
Electrophoresis of digested [PSB1K3] Kr(EP), [PSB1C3] Cr(EP),[PSB1A3] Ar(EP) (G1)
Cultivation of [PSB1C3] Cr (G1)
4/29
Mini-prep of [PSB1C3] Cr (G1)
Mini-prep of [PSB1C3] Cr (G2)
Transformation of [B0032] Ar, and [BFP] Cr (G2)
Cultivation of [PSB1K3] Kr, [PSB1A3] Ar (G1)
Cultivation of [B0032] Ar, and [BFP] Cr (G2)
4/30
Mini-prep of [PSB1A3] Ar (G1)
Mini-prep of [B0032] Ar, and [BFP] Cr (G2)
Transformation of [B0032] Ar, and [BFP] Cr, [T7promoter] Ar Kr and [T7 polymerase] Cr (G2)
Transformation of [J23101] Ar,[K592100] Cr,[B0032] Ar,[B0015] Ar (G1)
Digestion of [PSBIC3] Cr(EP), [B0032] Ar(ES), [BFP] Cr(XP) mini (G2)
Electrophoresis of plasmid and digested prouct of[PSBIC3] Cr(EP), [B0032] Ar(ES), [BFP] Cr(XP) mini (G2)