Difference between revisions of "Team:UiOslo Norway/Experiments/Alkaline Phospatase"
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<p> | <p> | ||
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| + | Following the provided <a href="http://www.biomart.cn/upload/asset/attachment/fermentas%E4%B8%AD%E5%9B%BD/coa_ef0511.pdf" > | ||
| + | protocol. | ||
| + | </a> | ||
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2 μl 10X Reaction Buffer</br> | 2 μl 10X Reaction Buffer</br> | ||
1 μl shrimp alkaline phosphatase</br> | 1 μl shrimp alkaline phosphatase</br> | ||
Revision as of 13:21, 19 August 2015
Shrimp Alkaline Phosphatase Treatment (Fermentas):
Back to ProtocolsFollowing the provided protocol. 2 μl 10X Reaction Buffer 1 μl shrimp alkaline phosphatase x μl DNA( 1 μg total concentration) MilliQ up to 20 μl
Total Reaction of 20 μl
Incubate at 37 °C for 30 minutes Heat inactivation at 65 °C for 15 minutes.