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Revision as of 16:49, 20 August 2015
- Project
- Modeling
- Lab
- Human
Practices - Parts
- About Us
Part Documentation
Each team will make new parts during iGEM and will submit them to the Registry of Standard Biological Parts. The iGEM software provides an easy way to present the parts your team has created. The <groupparts> tag (see below) will generate a table with all of the parts that your team adds to your team sandbox.
Remember that the goal of proper part documentation is to describe and define a part, so that it can be used without needing to refer to the primary literature. Registry users in future years should be able to read your documentation and be able to use the part successfully. Also, you should provide proper references to acknowledge previous authors and to provide for users who wish to know more.
Note
Note that parts must be documented on the Registry. This page serves to showcase the parts you have made. Future teams and other users and are much more likely to find parts by looking in the Registry than by looking at your team wiki.
Adding parts to the registry
You can add parts to the Registry at our Add a Part to the Registry link.
We encourage teams to start completing documentation for their parts on the Registry as soon as you have it available. The sooner you put up your parts, the better you will remember all the details about your parts. Remember, you don't need to send us the DNA sample before you create an entry for a part on the Registry. (However, you do need to send us the DNA sample before the Jamboree. If you don't send us a DNA sample of a part, that part will not be eligible for awards and medal criteria.)
What information do I need to start putting my parts on the Registry?
The information needed to initially create a part on the Registry is:
- Part Name
- Part type
- Creator
- Sequence
- Short Description (60 characters on what the DNA does)
- Long Description (Longer description of what the DNA does)
- Design considerations
We encourage you to put up much more information as you gather it over the summer. If you have images, plots, characterization data and other information, please also put it up on the part page.
Inspiration
We have a created a collection of well documented parts that can help you get started.
You can also take a look at how other teams have documented their parts in their wiki:
<groupparts>iGEM015 Example</groupparts>Registry parts
Part Name |
Description |
Registry Number |
| INP-EYFP | Fusion of Ice Nucleation Protein (INP) and Enhanced Yellow Fluorescent Protein (EYFP) | BBa_K523013 |
| cI | cI repressor from E. coli phage lambda modified with an LVA tail for rapid degradation of the protein | BBa_C0051 |
| lacI + LVA | lacI repressor from E. coli (+LVA) | BBa_C0012 |
| HylA | HlyA-tag+Secretion system, allows a protein to be secreted by means of the alpha-hemolysin secretion system in E. coli | BBa_K1166002 |
| pLuxR | Promoter activated by the LuxR protein complexed with the autoinducer homoserine lactone (HSL) | BBa_R0062 |
| pLldR operon | lldPRD operon promoter + RBS from E. coli. In the absence of L-lactate, lldR binds to two operator sites O1 and O2 in the promoter region and inhibits expression | BBa_K822000 |
| InterLab 1 strong promoter | Anderson promoter J23101 from the Anderson collection | BBa_K823005 (BBa_J23101) |
| InterLab 2 medium-strong promoter | Anderson promoter J23106 from the Anderson collection | BBa_K823008 (BBa_J23106) |
| terminator | Transcription terminator for the E.coli RNA polymerase | BBa_B0012 |
| double terminator | Double terminator consisting of BBa_B0010 and BBa_B0012 | BBa_B0015 |
| very strong promoter | Strong member of the family of promoters J23100 through J23119 | BBa_J23100 |
| gfp (InterLab) | intermediate in screening plasmid construction containing GFP | BBa_I13504 |
| medium promoter | Medium member of the family of promoters J23100 through J23119 | BBa_J23118 |
| aiiA | autoinducer inactivation enzyme aiiA (no LVA, an enzyme that inactivates the acylhomoserine lactone (AHL) quorum-sensing signal | BBa_C0160 |
| LuxI | autoinducer synthetase for acylhomoserine lactone (AHL), no LVA | BBa_C0161 |
| InterLab 3 weak promoter | Anderson promoter J23117 from the Anderson collection | BBa_K823013 (BBa_J23117) |
| promoter medium-weak | Medium-weak member of the family of promoters J23100 through J23119 | BBa_J23114 |
| RBS | RBS.3 (medium) derivative of BBa_0030 | BBa_B0032 |
| promoter plus GFP (InterLab control) | J23151 inserted in the Promoter MeasKit | BBa_I20270 |
| terminator | Artificial terminator, estimated %T~>90% | BBa_B1006 |
New constructs
Part Name |
Description |
Registry Number |
| Plasmid 3: | Combination of lldR operator sites O1 and O2 and promoters of variable potency. Gene expression is blocked by LldR protein (expressed separately) and induced upon addition of L-lactate. This was tested with a fusion to GFP. | |
| PL3a | lldRO1-plldR-lldRO2 | |
| PL3b | J23100-lldRO1-lldRO2 | |
| PL3c | J23100-lldRO1-R2oDNA-lldRO2 | |
| PL3d | J23117-lldRO1-lldRO2 | |
| PL3e | J23117-lldRO1-R2oDNA-lldRO2 | |
| PL3f | J23118-lldRO1-lldRO2 | |
| PL3g | J23118-lldRO1-R2oDNA-lldRO2 | |
| PL3h | lldRO1-J23100-lldRO2 | |
| PL3i | lldRO1-J23117-lldRO2 | |
| PL3j | lldRO1-J23118-lldRO2 | |
| PL3k | lldRO1-R2oDNA-lldRO2 |