Difference between revisions of "Team:FAFU-CHINA"
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| − | + | {{Template:Team:Yale2014/Templates/CSS}} | |
| + | {{Template:Team:Yale2014/Templates/Header}} | ||
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| − | < | + | <link href='http://fonts.googleapis.com/css?family=Montserrat:400,700' rel='stylesheet' type='text/css'> |
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| − | + | h1,h2,h3,h4,h5,h6{ | |
| − | <!-- | + | font-family:'Roboto'; |
| − | <table | + | } |
| − | <tr | + | |
| − | <td | + | #top-section{ |
| − | < | + | width:100%; |
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| + | position:absolute; | ||
| + | width:100px; | ||
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| + | left:720px; | ||
| + | top:550px; | ||
| + | } | ||
| + | </style> | ||
| + | |||
| + | <!--main content --> | ||
| + | <table width="1100px" align="center" style="margin-top:0px"> | ||
| + | <!--welcome box --> | ||
| + | <tr> | ||
| + | <td style="border:none;" colspan="3" align="center" height="150px"> | ||
| + | <h1 style="display:none">Biosynthesis of an Anti-biofouling Surface Binding Polymer with the 21st Amino Acid- L-DOPA</h1> | ||
| + | <br> | ||
| + | <p> <img src="https://static.igem.org/mediawiki/2014/d/d7/Ampersand_for_Yale_iGEM-v2.png" width="1100"> <div id = "vidbutton"></div></p> | ||
| + | </td> | ||
| + | </tr> | ||
| + | <!-- end welcome box --> | ||
| + | <tr><td colspan="3"><div class = "well" style="margin-bottom:10px"><p> <strong><center>Producing a Novel Antimicrobial Surface-Binding Peptide Using an Improved T7 Expression System</center></strong><p> | ||
| + | Biofilm formation on surfaces is an issue in the medical field, naval industry, and other areas. We developed an anti-fouling peptide with two modular components: a mussel adhesion protein (MAP) anchor and LL-37, an antimicrobial peptide. MAPs can selectively attach to metal and organic surfaces via L-3,5-dihydroxyphenylalanine (L-DOPA), a nonstandard amino acid that was incorporated using a genetically recoded organism (GRO). Because this peptide is toxic to the GRO in which it is produced, we designed a better controlled inducible system that limits basal expression. This was achieved through a novel T7 riboregulation system that controls expression at both the transcriptional and translational levels. This improved system is a precise synthetic switch for the expression of cytotoxic substances in the already robust T7 system. Lastly, the antimicrobial surface-binding peptide was assayed for functionality. | ||
| + | </p></div></td> | ||
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| − | </div> | + | <tr><div id = "show"><td colspan="3" style="padding-bottom:10px"><center><img src="https://static.igem.org/mediawiki/2014/5/56/Ampersand.png" width="150"></center></td></div></tr> |
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| + | <tr><td colspan="3"><a href="https://2014.igem.org/Team:Yale/Project"><img src=" | ||
| + | https://static.igem.org/mediawiki/2014/f/f8/Yale-Overview.png" width="1100"></a></td></tr> | ||
| − | < | + | <tr><td colspan="3"><a href="https://2014.igem.org/Team:Yale/Team"><img src="https://static.igem.org/mediawiki/2014/5/50/Yale-Team.png" width="1100"></a></td></tr> |
| − | https://2014.igem.org/ | + | |
| − | https://2014.igem.org/ | + | <tr><td colspan="3"><a href="https://2014.igem.org/Team:Yale/Outreach"><img src="https://static.igem.org/mediawiki/2014/0/08/Yale_Outreach.png" width="1100"></a></td></tr> |
| − | https://2014.igem.org/ | + | |
| + | <tr><td colspan="3"><a href="https://2014.igem.org/Team:Yale/Interlab"><img src="https://static.igem.org/mediawiki/2014/3/3c/Yale_Interlab.png" width="1100"></a></td></tr> | ||
| + | |||
| + | <tr><td colspan="3"><a href="https://2014.igem.org/Team:Yale/Notebook"><img src="https://static.igem.org/mediawiki/2014/2/2c/Yale_Notebook.png" width="1100"></a></td></tr> | ||
| + | |||
| + | <tr><td colspan="3"><a href="https://2014.igem.org/Team:Yale/Achievements"><img src="https://static.igem.org/mediawiki/2014/2/29/Yale_Achivements.png" width="1100"></a></td></tr> | ||
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| + | </table> | ||
| + | |||
| + | <div width="1100px" align="center" > | ||
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| + | </div> | ||
| + | </html> | ||
| + | {{Template:Team:Yale2014/Templates/Footer}} | ||
Revision as of 19:43, 21 August 2015
Template:Team:Yale2014/Templates/CSS Template:Team:Yale2014/Templates/Header
Biosynthesis of an Anti-biofouling Surface Binding Polymer with the 21st Amino Acid- L-DOPA |
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Biofilm formation on surfaces is an issue in the medical field, naval industry, and other areas. We developed an anti-fouling peptide with two modular components: a mussel adhesion protein (MAP) anchor and LL-37, an antimicrobial peptide. MAPs can selectively attach to metal and organic surfaces via L-3,5-dihydroxyphenylalanine (L-DOPA), a nonstandard amino acid that was incorporated using a genetically recoded organism (GRO). Because this peptide is toxic to the GRO in which it is produced, we designed a better controlled inducible system that limits basal expression. This was achieved through a novel T7 riboregulation system that controls expression at both the transcriptional and translational levels. This improved system is a precise synthetic switch for the expression of cytotoxic substances in the already robust T7 system. Lastly, the antimicrobial surface-binding peptide was assayed for functionality. |
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