Difference between revisions of "Team:UCLA/Notebook/Protein Cages/21 August 2015"
| Line 16: | Line 16: | ||
OD after 3.5 hours: 0.443 | OD after 3.5 hours: 0.443 | ||
| + | |||
| + | OD after 4.5 hours: 1.091 | ||
| + | |||
| + | Induced with IPTG to 1mM concentration at ~6:20pm. | ||
Revision as of 01:29, 22 August 2015
Intro: Grew the starter culture overnight for ~16 hours. Refreshed into 300 mL expression culture. Induced with IPTG.
Expression Culture:
OD with 4 mL of starter culture: 0.023
OD with all of starter culture: 0.049
Incubation with all of starter culture began at 1:25 pm.
OD after one hour: 0.073
OD after two hours: 0.112
OD after 3.5 hours: 0.443
OD after 4.5 hours: 1.091
Induced with IPTG to 1mM concentration at ~6:20pm.