Difference between revisions of "Team:Czech Republic/Project/Communication module"
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=== DNA === | === DNA === | ||
| + | * Genomic PCR of WT SC-STE2 (YFL026W - http://www.yeastgenome.org/locus/S000001868/overview) - ORF | ||
| + | * Genomic PCR of MF(ALPHA)1 (YPL187W - http://www.yeastgenome.org/locus/mf%28alpha%291/overview) - secretion tag (first PCR), second PCR to add the actual pheromone and stop codon | ||
== Materials and methods == | == Materials and methods == | ||
Revision as of 13:47, 26 August 2015
Intro / Background
Test [Janiak2005].
Design
Concept
DNA
- Genomic PCR of WT SC-STE2 (YFL026W - http://www.yeastgenome.org/locus/S000001868/overview) - ORF
- Genomic PCR of MF(ALPHA)1 (YPL187W - http://www.yeastgenome.org/locus/mf%28alpha%291/overview) - secretion tag (first PCR), second PCR to add the actual pheromone and stop codon
Materials and methods
Chemicals and strains
Construction
Validation
Results
Proof of concept test
Test of orthogonality (growth arrest approach)
Test of orthogonality (fluorescence approach)
Final constructs
References
- ↑ Janiak, A. M., Sargsyan, H., Russo, J., Naider, F., Hauser, M., & Becker, J. M. (2005). Functional expression of the Candida albicans α-factor receptor in Saccharomyces cerevisiae. Fungal Genetics and Biology, 42(2005), 328–338. doi:10.1016/j.fgb.2005.01.006