Difference between revisions of "Team:Evry/Notebook"

(Added some example data to explain how the notebook has to be completed!)
(Added the first entry !!!)
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Aliquam eget fringilla turpis. Phasellus vel massa ac lacus faucibus cursus. Nunc laoreet gravida metus, non varius mi egestas sed. Phasellus efficitur, nulla sed vehicula aliquam, mauris orci fringilla purus, eget dictum sem ante quis dolor. Integer gravida elementum lacus ac pharetra. Etiam vel neque nec urna semper pellentesque non et ex. Donec et accumsan sem, a viverra mauris. Maecenas sed ligula id libero auctor lacinia ac at orci. Quisque blandit lorem eu maximus sagittis. Curabitur ullamcorper ac nulla at ultrices. Fusce vitae molestie nibh, in aliquet justo.
 
</p>
 
</p>
 +
<div id="secretion_06_12" class="notebook-entry">
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<h4>Friday, 12<sup>th</sup> June 2015</h4>
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<p class="text-justify">
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<strong>Extraction of AgA1P from yeast genome with BSAI</strong>
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<br>
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AGA1P was extracted with BSAI overhangs for subsequent cloning from W303 and BY4000, according to <em>Looke et al., PMC 2011</em>.
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</p>
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<p class="text-justify">
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<ol>
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    <li> Resuspend one yeast colony in 100 µl of 200 mM LiAc, 1% SDS solution and incubate at 70°C</li>
 +
    <li>Add 300 µl of 36% ethanol and vortex</li>
 +
    <li>Spin down DNA at 15 000 g for 3 minute</li>
 +
    <li>Wash pellet with 70% ethanol</li>
 +
    <li>Disolve pellet in 100 µl of water and spin down debris at 15 000 g for 15 seconds</li>
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</ol>
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</p>
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<p class="text-justify">
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<strong>PCR of AGA1P with primers AGA1P R1/F1 with Q5 polymerase using a gradient (57/60/63°C)</strong></p>
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<p class="text-justify"><span class="text-primary">Q5 PCR Program</span>
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<ul>
 +
    <li>step 1 : 98°C – 30 seconds</li>
 +
    <li>step 2 : 98°C – 10 seconds</li>
 +
    <li>step 3 : 57, 60 or 63°C</li>
 +
    <li>step 4 : 72°C – 1 min (repeat steps 2-4 for 40 cycles)</li>
 +
    <li>step 5 : 16°C - Hold</li>
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</ul>
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<hr>
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</div>
 
<div id="project_month_day">
 
<div id="project_month_day">
 
     <h3>Example notebook entry</h3>
 
     <h3>Example notebook entry</h3>

Revision as of 17:19, 26 August 2015

Notebook

Here is our lab notebook. Follow all the wet lab experiments we did, day by day.

  • Yeast surface-display
  • Yeast encapsulation
    • May
      • Week 1
      • Week 2
      • Week 3
      • Week 4
  • Hypoxia bio-sensor
    • May
      • Week 1
      • Week 2
      • Week 3
      • Week 4
  • MAIT cells stimulation
    • May
      • Week 1
      • Week 2
      • Week 3
      • Week 4
  • Antigene prediction
    • May
      • Week 1
      • Week 2
      • Week 3
      • Week 4

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Friday, 12th June 2015

Extraction of AgA1P from yeast genome with BSAI
AGA1P was extracted with BSAI overhangs for subsequent cloning from W303 and BY4000, according to Looke et al., PMC 2011.

  1. Resuspend one yeast colony in 100 µl of 200 mM LiAc, 1% SDS solution and incubate at 70°C
  2. Add 300 µl of 36% ethanol and vortex
  3. Spin down DNA at 15 000 g for 3 minute
  4. Wash pellet with 70% ethanol
  5. Disolve pellet in 100 µl of water and spin down debris at 15 000 g for 15 seconds

PCR of AGA1P with primers AGA1P R1/F1 with Q5 polymerase using a gradient (57/60/63°C)

Q5 PCR Program

  • step 1 : 98°C – 30 seconds
  • step 2 : 98°C – 10 seconds
  • step 3 : 57, 60 or 63°C
  • step 4 : 72°C – 1 min (repeat steps 2-4 for 40 cycles)
  • step 5 : 16°C - Hold

Example notebook entry

I am a big text

i am a justified text.

On the left

Center

i am a green text on the right. 

I am a fixed-width text
I am some code I am a link
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