Difference between revisions of "Team:UCLA/Notebook/Honeybee Silk/19 May 2015"
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*Froze down the pellets in the falcon tubes at -80C | *Froze down the pellets in the falcon tubes at -80C | ||
*Next up, will lyse the cells and collect protein with BugBuster | *Next up, will lyse the cells and collect protein with BugBuster | ||
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| + | ===Transformation=== | ||
Revision as of 20:37, 19 May 2015
5/19
Cell Harvesting
- Cells were grown up in 250 ml of LB medium and induced with IPTG. See 5/18 for details.
- The Cells were split up into 5 pre weighed 50 ml falcon tubes, with 45 ml of cells in each tube.
- I should have taken the OD 600 at this point, but I forgot to.
- Mass Balanced the tubes and added a water tube, and spun down the cells at 5300 rpm at 4 C for 15 min. to pellet the cells.
- Decanted the supernatant.
- Weighed the pellets
- In total, the wet weight of all the pellets across all 5 falcon tubes was 2.42 grams, approximately 0.5 grams per 45 ml of culture.
- Froze down the pellets in the falcon tubes at -80C
- Next up, will lyse the cells and collect protein with BugBuster