Difference between revisions of "Team:NEFU China/fit"
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<div class="col-sm-8 blog-main"> | <div class="col-sm-8 blog-main"> | ||
| − | < | + | <div class="blog-post"> |
| − | <div class="blog-post"> | + | <h2 class="blog-post-title">pNZ8148</h2> |
| − | <h2 class="blog-post-title"></h2> | + | <p class="blog-post-meta"> by <a href="#">Reage Yao</a></p> |
| − | <p class="blog-post-meta"> by <a href="#"></a></p> | + | <p><img alt="" src="http://igem-uploads.stor.sinaapp.com/2015-03-06/54f93b5e1dce8.png" style="height:514px; width:608px" /></p> |
| − | + | ||
| − | + | <p>乳酸杆菌表达载体</p> | |
| + | |||
| + | <p><span style="color:rgb(68, 68, 68); font-family:arial,helvetica,sans-serif; font-size:12px">The pNZ8148 vector contains an origin of replication (ORI), the gene for the resistance to chloramphenicol, two genes for the replication proteins repA and repC, the nisin-inducible promoter (P nisA), and the transcription terminator (T). The gene, tagged with a Strep-tag II (STREP) followed by a stop codon (*), can be inserted between the necessary NcoI site and another endonuclease site from the multicloning site (MCS) such as PstI, SphI, KpnI, SpeI, XbaI, SacI and HindIII.</span></p> | ||
| + | </div><!-- /.blog-post --><div class="blog-post"> | ||
| + | <h2 class="blog-post-title">pBBR-1-MCS-5</h2> | ||
| + | <p class="blog-post-meta"> by <a href="#">reageyao</a></p> | ||
| + | <p><img alt="" src="http://igem-uploads.stor.sinaapp.com/2015-03-06/54f93f716091f.png" style="height:286px; width:348px" /></p> | ||
| + | </div><!-- /.blog-post --><div class="blog-post"> | ||
| + | <h2 class="blog-post-title">Mini-prep</h2> | ||
| + | <p class="blog-post-meta"> by <a href="#">王威</a></p> | ||
| + | <p>Today I have condulted a mini-prep.However,the result is not satisfying at all,for the concentration of the plasmid are all no more than 100ng/μl.</p> | ||
| + | </div><!-- /.blog-post --> </div> | ||
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<ul class="pager"> | <ul class="pager"> | ||
Revision as of 16:23, 28 August 2015
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pNZ8148
乳酸杆菌表达载体
The pNZ8148 vector contains an origin of replication (ORI), the gene for the resistance to chloramphenicol, two genes for the replication proteins repA and repC, the nisin-inducible promoter (P nisA), and the transcription terminator (T). The gene, tagged with a Strep-tag II (STREP) followed by a stop codon (*), can be inserted between the necessary NcoI site and another endonuclease site from the multicloning site (MCS) such as PstI, SphI, KpnI, SpeI, XbaI, SacI and HindIII.
pBBR-1-MCS-5
Mini-prep
Today I have condulted a mini-prep.However,the result is not satisfying at all,for the concentration of the plasmid are all no more than 100ng/μl.