Difference between revisions of "Team:HSNU-TAIPEI/Project/Benzo a pyrene"
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| − | <h1><font color="#FF8000"><strong>Benzo[a]pyrene</strong></font></h1> | + | <h1><font color="#FF8000"> <strong>Benzo[a]pyrene</strong></font></h1> |
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| − | < | + | <h2><font color="#008b8b"> ✡ Introduction</font></h2> |
| − | + | <p><font face="Comic Sans MS"> (1)Why do we detect benzo[a]pyrene?</font></p> | |
| − | + | <p><font face="Comic Sans MS"> Because when food,such as fried food ,which is heated above 300 degrees</font></p> | |
| − | + | <p><font face="Comic Sans MS"> celcius,food will release benzo[a]pyrene recycled oil usually contain</font></p> | |
| − | </ | + | <p><font face="Comic Sans MS"> benzo[a]pyrene[1].</font></p> |
| + | <p><font face="Comic Sans MS"> (2)Harm?</font></p> | ||
| + | <p><font face="Comic Sans MS"> Pathway:from skin,breathing in,eating | ||
| + | Carcinogenic,environmental</font></p> | ||
| + | <p><font face="Comic Sans MS"> pollution, Skin irritation,eye irritation[1].</font></p> | ||
| + | <p><font face="Comic Sans MS"> (3)Taiwanese regulation</font></p> | ||
| + | <img src="https://static.igem.org/mediawiki/2015/2/21/2015hsnu-benzo-a-pyrene_1.png" height="250" width="550"> | ||
| + | <p><font face="Comic Sans MS"> 食用油脂類衛生標準第二條：重金屬及芥酸之最大容許量：汞0.05 ppm</font></p> | ||
| + | <p><font face="Comic Sans MS"> 食米重金屬限量標準第二條：食米中重金屬含量應符合以下限量：0.05ppm。</font></p> | ||
| + | </div> | ||
| + | <div> | ||
| + | <h2><font color="#008b8b"> ✡ Circuit Design</font></h2> | ||
| + | <img src="https://static.igem.org/mediawiki/2015/7/7e/2015hsnu-mercury1.png" height="250" width="400"> | ||
| + | <p><font face="Comic Sans MS"> MerR可以和汞結合然後改變DNA的結構好讓RNA聚合酶方便轉入。</font></p> | ||
| + | <p><font face="Comic Sans MS"> PmerT可以被MerR控制。PmerT步行進行轉錄是因為MerR會改變PmerT的</font></p> | ||
| + | <p><font face="Comic Sans MS"> 形狀。總而言之，只要汞與MerR結合，後續反應就可進行。(註3)(註4)</font></p> | ||
| + | <img src="https://static.igem.org/mediawiki/2015/b/b2/2015hsnu-mercury_2.png" height="250" width="550"> | ||
| + | </div> | ||
| + | <div> | ||
| + | <h2><font color="#008b8b"> ✡ Result</font></h2> | ||
| + | </div> | ||
| + | <div> | ||
| + | <h2><font color="#008b8b"> ✡ Reference</font></h2> | ||
| + | <p><font face="Comic Sans MS"> (註1)Metabolism of mercury, administered as methylmercuric chloride or mercuric chloride, by lactating ruminants.</font></p> | ||
| + | <p><font face="Comic Sans MS"> (註2)台北醫學大學公共衛生學系碩士班碩士論文Graduate Institute.</font></p> | ||
| + | <p><font face="Comic Sans MS"> (註3)Brown, N. L., J. V. Stoyanov, et al. (2003). "The MerR family of transcriptional regulators." FEMS Microbiol Rev 27(2-3): 145-163.</font></p> | ||
| + | <p><font face="Comic Sans MS"> (註4)Park, S. J., J. Wireman, et al. (1992). "Genetic analysis of the Tn21 mer operator-promoter." J Bacteriol 174(7): 2160-2171.</font></p> | ||
| + | </div> | ||
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Revision as of 13:18, 29 August 2015
Benzo[a]pyrene
✡ Introduction
(1)Why do we detect benzo[a]pyrene?
Because when food,such as fried food ,which is heated above 300 degrees
celcius,food will release benzo[a]pyrene recycled oil usually contain
benzo[a]pyrene[1].
(2)Harm?
Pathway:from skin,breathing in,eating Carcinogenic,environmental
pollution, Skin irritation,eye irritation[1].
(3)Taiwanese regulation
食用油脂類衛生標準第二條：重金屬及芥酸之最大容許量：汞0.05 ppm
食米重金屬限量標準第二條：食米中重金屬含量應符合以下限量：0.05ppm。
✡ Circuit Design
MerR可以和汞結合然後改變DNA的結構好讓RNA聚合酶方便轉入。
PmerT可以被MerR控制。PmerT步行進行轉錄是因為MerR會改變PmerT的
形狀。總而言之，只要汞與MerR結合，後續反應就可進行。(註3)(註4)
✡ Result
✡ Reference
(註1)Metabolism of mercury, administered as methylmercuric chloride or mercuric chloride, by lactating ruminants.
(註2)台北醫學大學公共衛生學系碩士班碩士論文Graduate Institute.
(註3)Brown, N. L., J. V. Stoyanov, et al. (2003). "The MerR family of transcriptional regulators." FEMS Microbiol Rev 27(2-3): 145-163.
(註4)Park, S. J., J. Wireman, et al. (1992). "Genetic analysis of the Tn21 mer operator-promoter." J Bacteriol 174(7): 2160-2171.