Difference between revisions of "Team:Toulouse/Practices"

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<div class="wrapper row2 bgded" style="background-image:url('https://static.igem.org/mediawiki/2015/0/03/TLSE_bakcground.png');">
 
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    <div id="pageintro">
 
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<div class="title">
 
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      <h2>Context</h2>
 
  
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      <ul>
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.row1, .row1 a{}
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        <li><a href="#domBee">1- Domestic bees</a></li>
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.row2, .row2 a{}
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        <li><a href="#CCD">2- Collapse of Bees</a></li>
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.row3, .row3 a{}
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        <li><a href="#varroa"><i>3- Varroa destructor</i></a></li>
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        <li><a href="#outB">4- Outbreak of Varroa</a></li>
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<li><a href="#fight">5- Means to fight Varroa</a></li>
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    <center> <h3>Practices</h3> </center>
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<div class="group center">
 
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<p align="justify" style="font-size:15px;">
 
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The iGEM competition is not all about wet lab and finding sponsors. To breath life into a project
 
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public contact is paramout.
 
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When we first started to reach out to the public we realised that the decline in bee population was
 
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a well known issue, in spite of this, most of the people interviewed weren't aware of the <i>Varroa destructor</i> influence.
 
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</p>
 
−
</div>
 
−
<div class="group center">
 
−
<div class="one quarter first">
 
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<div class="subtitle" style="margin-bottom:0px;">  
 
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<h3>ExpoSciences Midi-Pyrénées
 
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</h3>
 
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</div>
 
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<a href="#"><img src="https://static.igem.org/mediawiki/2015/5/57/TLSE_Attract_BG.png" style="width:100%;" /></a>
 
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−
<div class="one quarter">
 
−
<div class="subtitle" style="margin-bottom:0px;">  
 
−
<h3>Communication in high school
 
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</h3>
 
−
</div>
 
  
−
<a href="#"><img src="https://static.igem.org/mediawiki/2015/5/57/TLSE_Attract_BG.png" style="width:100%;" /></a>
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<h3>ExpoSciences International
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−
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<h3>Looking forward
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</h3>
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 +
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<center><div class="subtitle" >  
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<h3>ExpoSciences Midi-Pyrénées</h3>
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 +
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<p align="justify" style="font-size:15px;">
 
−
Our team took part to regional ExpoSciences in Toulouse.
 
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This event brings together scientists of all ages and levels
 
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who want to share scientific knowledges. It’s a good exercise
 
−
which enables to explain what’s synthetic biology.
 
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Given that DNA is our main work tool, we decided to let the children try out banana
 
−
DNA extraction, with ethanol and dishwashing soap.
 
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The stand having drawn everybody’s attention,
 
−
our intervention has been broadcasted on a local TV (TLT),
 
−
and we also have been selected to take part to International ExpoSciences.
 
−
</p>
 
−
</div>
 
  
−
<div class="subtitle" style="margin-bottom: 0px;">  
 
−
<h3 style="font-size:20px;">Recipe for a successful banana DNA extraction!</h3>
 
−
</div>
 
  
−
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+
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 +
---------------------------------------------------------------------------------------------------------------*/
 +
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 +
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−
<tr>
 
−
    <td>
 
  
−
<ul style="font-size:15px;margin-bottom:10px;">
+
/* Back to Top
−
<li>
+
---------------------------------------------------------------------------------------------------------------*/
−
A ripe banana (Brownish)
+
−
</li>
+
−
<li>
+
−
50 ml of water (About a half cup)
+
−
</li>
+
−
<li>
+
−
2 teaspoon of salt
+
−
</li>
+
−
<li>
+
−
2 teaspoon Dishwashing soap or detergent
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−
</li>
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−
<li>
+
−
Rubbing alcohol (cold)
+
−
</li>
+
−
<li>
+
−
Coffee filter
+
−
</li>
+
−
<li>
+
−
A test tube (or narrow glass)
+
−
</li>
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−
<li>
+
−
Narrow wooden stirrer
+
−
</li>
+
−
</ul>
+
  
−
</td>
 
−
<td>
 
  
−
<ul style="font-size:15px;margin-bottom:10px;">
+
/* Transition Fade
−
<li>
+
--------------------------------------------------------------------------------------------------------------- */
−
Peel and smash half of the Banana into a pulp
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</li>
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−
<li>
+
−
Add the 50ml of water(must be at ambient temperature), the salt and the Dishwashing soap
+
−
</li>
+
−
<li>
+
−
Stir until a soft foam starts to appear
+
−
</li>
+
−
<li>
+
−
Let the mix pour through the coffee filter (about five minutes)
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−
</li>
+
−
<li>
+
−
Throw out the coffee filter and its content, keep the liquid
+
−
</li>
+
  
−
<li>
 
−
Take a 2ml sample and pour it in the test tube
 
−
</li>
 
  
−
<li>
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/*-------------------------------------------------------------------------------------------------------------*/
−
Add 4ml of alcohol, two layer should form in the test tube
+
/*-------------------------------------------------------------------------------------------------------------*/
−
</li>
+
/*-------------------------------------------------------------------------------------------------------------*/
 +
/*-------------------------------------------------------------------------------------------------------------*/
 +
/*-------------------------------------------------------------------------------------------------------------*/
  
−
<li>
 
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In the alcohol layer, a white cloud with bubbles should appear, this cloud is DNA!
 
−
</li>
 
−
</ul>
 
  
−
<br>
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/* Navigation
−
</td>
+
---------------------------------------------------------------------------------------------------------------*/
−
<td>
+
nav ul, nav ol{margin:0; padding:0; list-style:none;}
  
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<img src="https://static.igem.org/mediawiki/2015/4/40/TLSE_Practices_pic1.png" style="width:400px;"/>
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−
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−
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−
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 +
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<p align="justify" style="font-size:15px;">
+
/* Breadcrumb */
−
This simple, yet interesting experience helped us attract children to our stand. It was a great starting point
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#breadcrumb{display:block; padding:15px 20px; border-radius:5px 5px 0 0;}
−
for us we managed to start some indepth conversation with them about what they knew of DNA and what could be done with it.
+
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Some of them weren't aware that banana had DNA at all and some teacher told us that our demonstration was very well though up and
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easy to understand without distorting the truth.<br>
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 +
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 +
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We were the first most visited stand in the expo (thanks to the pungent smell of smashed banana) and
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/* Sidebar Navigation */
−
our intervention has even been broadcasted on a local TV (TLT[1]).
+
.sidebar nav{display:block; width:100%;}
−
our success had us selected to take part to the <b>International ExpoSciences</b> in Brussel!.
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.sidebar nav li{margin:0 0 3px 0; padding:0;}
−
</p>
+
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 +
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 +
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 +
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 +
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 +
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−
<center><div class="subtitle" >  
+
/* Forms
−
<h3>Communication in junior high school</h3>
+
---------------------------------------------------------------------------------------------------------------*/
−
</div></center>
+
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 +
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 +
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 +
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−
<div class="group center">
+
/* Comment Area */
−
<p align="justify" style="font-size:15px;">
+
#comments form{display:block; width:100%;}
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To carry on our education vibe, we met with the science teacher from the Leonard de Vinci junior high school of Tournefeuille to
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#comments input, #comments textarea{width:100%; padding:10px; border:1px solid;}
−
meet with the students. The plan? Bees, Varroas, an introduction to synthetic biology and, of course, the banana DNA extraction!
+
#comments textarea{overflow:auto;}
−
</p>
+
#comments div{margin-bottom:15px;}
−
</div>
+
#comments input[type="submit"], #comments input[type="reset"]{display:inline-block; width:auto; min-width:150px; margin:0; padding:8px 5px; cursor:pointer;}
  
  
−
<center><img src="https://static.igem.org/mediawiki/2015/7/73/TLSE_Practices_pic2.JPG" style="width:80%;"/></center>
+
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 +
---------------------------------------------------------------------------------------------------------------*/
 +
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 +
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−
<div class="group center">
 
−
<div class="one_half first">
 
−
<div class="subtitle" style="margin-bottom: 0px;">  
 
−
<h3 style="font-size:15px;">Teatching about varroas and bees</h3>
 
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</div>
 
−
<p align="justify" style="font-size:15px;">
 
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We prepared a short presentation on varroa and organised a question and answer with the pupils about the disappearance of bees,
 
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pesticides, chemical treatments and varroa. The presentation was followed by a Q and A session where student were able to interact with us and
 
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each other about varroa and synthetic biology. With the help of the teacher we were able to explain the basics of synthetic biology, the idea of biobricks
 
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and spark a quick debate on GMO use. Most of the children were not antagonized by the use of a modified bacterium as long as it were confined in our Trapicoli,
 
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but we felt that the biological knowledge needed to fully understand synthetic biology was a little foreign for them, so we switched our lecture to be
 
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more about bees and the colony collapse disorder.<br>
 
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</p>
 
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</div>
 
−
<div class="one_half">
 
−
<div class="subtitle" style="margin-bottom: 0px;">  
 
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<h3 style="font-size:15px;">An unexpected advice</h3>
 
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</div>
 
  
−
<p align="justify" style="font-size:15px;">
+
/* Gallery
−
During the Q and A, we were asked about our studies and what kind of diploma we had. Question led to another
+
---------------------------------------------------------------------------------------------------------------*/
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and without realising we where already doing a full guidance lecture about the choices these children will have to do in the following years (which school to
+
#gallery{display:block; width:100%; margin-bottom:50px;}
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attend too and why, why should I study science? etc...). The point of view from students who had to go through those choices not so long ago helped them
+
#gallery figure figcaption{display:block; width:100%; clear:both;}
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gain assurance and ease their fear about their future studies. <br>
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#gallery li{margin-bottom:30px;}
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<br>
+
−
We hope that we helped create a few scientific vocations during this talks (or maybe a future iGEMer!)
+
−
</p>
+
  
 +
.overlay ul li a{display:block; position:relative;}
 +
.overlay ul li a::before{display:block; position:absolute; top:0; left:0; width:100%; height:100%; content:"";}
 +
.overlay ul li a::after{display:block; position:absolute; top:50%; left:50%; width:40px; height:40px; line-height:40px; margin:-18px 0 0 -18px; padding:0; border-radius:50%; font-family:"FontAwesome"; content:""; font-size:18px; text-align:center;}
 +
.overlay ul li a::before, .overlay ul li a::after{opacity:0;}
 +
.overlay ul li a:hover::before, .overlay ul li a:hover::after{opacity:1;}
  
−
</div>
 
  
 +
/* Font Awesome Social Icons
 +
---------------------------------------------------------------------------------------------------------------*/
 +
.faico{margin:40px; padding:0; list-style:none;}
 +
.faico li{display:inline-block; margin:0 2px 0 0; padding:0; line-height:normal;}
 +
.faico li:last-child{margin-right:0;}
 +
.faico a{display:inline-block; width:30px; height:30px; line-height:30px; border-radius:5px; font-size:16px; text-align:center;}
  
 +
.faico a{color:inherit; }
 +
.faico a:hover{color:#FFFFFF;}
  
−
</div>
+
.faicon-facebook:hover{background-color:#3B5998;}
−
+
.faicon-google-plus:hover{background-color:#DB4A39;}
−
<div class="group center">
+
.faicon-instagram:hover{background-color:#3F729B;}
−
<p align="justify" style="font-size:15px;">
+
.faicon-linkedin:hover{background-color:#0E76A8;}
−
The main aim of this intervention was to teach children about varroa and synthetic biology while creating interest for science in general.  
+
.faicon-tumblr:hover{background-color:#34526F;}
−
But for us it taught us how to adapt our speech too younger audience, we had to stop taking for granted notions such as genes and even DNA. The experience
+
.faicon-twitter:hover{background-color:#00ACEE;}
−
we acquired here was them reused and we became able to switch from a full science explanation to a simpler overview of our project without getting boring.  
+
−
</p>
+
−
</div>
+
  
−
<center><div class="subtitle" >  
 
−
<h3>Giant Jamboree training: International Exposciences in Brussels</h3>
 
−
</div></center>
 
  
−
<div class="group center">
+
/*-------------------------------------------------------------------------------------------------------------*/
−
<p align="justify" style="font-size:15px;">
+
/*-------------------------------------------------------------------------------------------------------------*/
−
The team had the wonderful opportunity to take part the International ExpoSciences in Brussels,
+
/*-------------------------------------------------------------------------------------------------------------*/
−
during this science fair we had the chance to meet people from all around the world to exchange
+
/*-------------------------------------------------------------------------------------------------------------*/
−
knowledge and ideas. In the middle our summer our project had matured a lot and the expo was
+
/*-------------------------------------------------------------------------------------------------------------*/
−
the occasion for us to test it in the real world exchanging with scientist. <br>
+
−
We also spent our time explaining the iGEM competition to visitors and participant alike
+
−
bringing light unto the synthetic biology field, still quite unknown.<br>
+
−
We also took the opportunity of being in belgium and organised a quick meetup with KU Leuven iGEM team.
+
−
This communication is fully integrated into the Apicoli project enhancing its exposure and credibility toward people.
+
−
Besides this experience enabled us to meet his Excellency mister Bernard Valero,
+
−
the ambassador of France in Belgium, and mister Jean-Claude Guiraudon, CIRASTI President and MILSET Honorary President, both of them
+
−
wished us luck on our endeavours.
+
−
</p>
+
−
</div>
+
  
−
<div class="group center">
 
−
<p align="justify" style="font-size:15px;">
 
−
Overall this experience in Brussels was quite intense, we met so many people interested in science and it was refreshing to be able to get out the lab
 
−
and develop our project on the field. We hope to feel the same engagement this September in Boston.
 
−
</p>
 
−
</div>
 
  
−
<center><div class="subtitle" >  
+
/* Colours
−
<h3>Looking forward!</h3>
+
---------------------------------------------------------------------------------------------------------------*/
−
</div></center>
+
body{color:black; background-color:#817E8B;}
  
−
<div class="group center">
+
hr, .borderedbox{border-color:#EDEDED;}
−
<p align="justify" style="font-size:15px;">
+
label span{color:#FF0000; background-color:inherit;}
−
With august looming over us, we had trouble organizing any education event. But iGEM does not stop the 28th of september, we have everything planned to
+
.btn{color:#FFFFFF;}
−
keep our project alive outside of the competition:
+
.font_xxl:after{color:inherit; background-color:rgba(9, 181, 31, 0.32);}
−
</p>
+
−
</div>
+
−
<ul style="font-size:15px;margin-bottom:10px;">
+
−
<li>
+
−
Semaine de l’étudiant : October 9-18 in Toulouse<br>
+
−
The "Student's week" occuring in several towns in the Midi-Pyrénées region facilitate
+
−
the integration of new student in Toulouse allowing them to participate in free event organized by
+
−
associations.
+
−
</li>
+
−
<li>
+
−
Scientilivre : October 17-18 <br>
+
−
Taking place in Toulouse Scientilivre ("Science book") is here to present both books and science
+
−
to the public. We plan to present our project Apicoli with posters and some playful experimentations for families.
+
−
</li>
+
−
</ul>
+
  
−
</div><!-- END OF ROW3-->
 
−
</main>
 
−
<!-- ################################################################################################ -->
 
−
<!-- ################################################################################################ -->
 
−
<!-- #######################        FOOTER        ######################################################################### -->
 
  
−
</html>{{Toulouse/footer}}<html>
+
/* Rows */
 +
/* Structure des rows, le row0 est la barre de nav */
 +
.row0{color:#101132; background-color:rgba(245, 245, 245, 1); border-color:rgba(29, 5, 79, 0.81); max-height:630px}
 +
.row0:hover{background-color:rgba(245, 245, 245, 1);}
 +
.row1, .row1 a{color:#FFFFFF; background-color:#000000;}
 +
.row2, .row2 a{color:#FFFFFF; background-color:#000000;}
 +
.row3{color:#888888; background-color:white;}
 +
.row4, .row4 a{color:#B4B4B4; background-color:#EDEDED;}
 +
.row5{color:white; background-color:#817E8B;}
 +
.row6, .row6 a{color:#B4B4B4; background-color:#817E8B;}
 +
.row6{border-color:#3C3C3C;}
  
  
 +
/* Header */
 +
#header #logo h1 a{color:inherit;}
  
−
</body>
+
 
 +
/* Shout */
 +
.shout .shout-content{color:inherit; background-color:rgba(251, 255, 245, 0.53);max-width:800px;max-height:300px;}
 +
.shout .shout-content:hover{background-color:rgba(247, 244, 255, 0.48);}
 +
 
 +
 
 +
/* Latest Work */
 +
.latest ul, .latest ul li{border-color:#EDEDED;}
 +
 
 +
 
 +
/* Content */
 +
.boxedicon a{color:#56AED4; }
 +
.boxedicon a:hover{color:#FFFFFF;}
 +
 
 +
 
 +
/* Footer */
 +
/* Couleur de la barre du footer */
 +
#footer .shout .shout-content, #footer .shout .shout-content:hover{color:rgba(8, 44, 47, 0.82); background-color:#EDEDED; border-bottom: 7px solid rgba(46, 29, 89, 0.88);}
 +
#footer .title{color:#FFFFFF; border-color:#3C3C3C;} /* Couleur des textes */
 +
#footer input{color:#FFFFFF; background-color:#3C3C3C;}
 +
#footer button{color:#FFFFFF;}
 +
e-block;}
 +
 
 +
/*Back to Top*/
 +
 
 +
 
 +
/* Navigation */
 +
 
 +
#mainav li a{color:inherit;} /* Couleur des textes de la barre nav */
 +
#mainav.active a, #mainav a:hover, #mainav li:hover > a{; background-color:inherit;} /* Couleur de fond lors d'un mouseover du texte */
 +
#mainav li li a, #mainav .active li a{color:black; background-color:rgba(236, 255, 226, 0.9); border-color:rgba(236, 255, 226, 0.6);} /* Gestion de la couleur des onglets */
 +
#mainav li li:hover > a, #mainav .active .active > a{color:#FFFFFF; background-color:rgba(29, 5, 79, 0.81);border-color:rgba(29, 5, 79, 0.81);font-size:12px;}/* Gestion couleur des onglets lors d'un mouseover */
 +
#mainav li a.set{color:#90A83E;font-size:15px;} /*classe active qui s'appelle pas active parceque active ?a marchait pas */
 +
/* J'ai toujours pas trop compris */
 +
#breadcrumb{color:inherit; background-color:rgba(255, 255, 255, 0.7);}
 +
#breadcrumb a{background-color:inherit;}
 +
 
 +
.container .sidebar nav a{color:inherit; border-color:#EDEDED;}
 +
.container .sidebar nav a:hover{color:#56AED4;}
 +
 
 +
.pagination a, .pagination strong{border-color:#EDEDED;}
 +
.pagination .current{color:#B9D94A;}
 +
 
 +
/*ALL TABLE ATTRIBUTES*/
 +
/* Tables + Comments
 +
df for default style table*/
 +
table.df th td, #comments .avatar, #comments input, #comments textarea{border-color:#EDEDED;}
 +
table.df th{color:#FFFFFF; background-color:#373737;}
 +
table.df tr, #comments li, #comments input[type="submit"], #comments input[type="reset"]{color:inherit; background-color:#FBFBFB;}
 +
table.df tr:nth-child(even), #comments li:nth-child(even){color:inherit; background-color:#F7F7F7;}
 +
table.df a, #comments a{background-color:inherit;}
 +
 
 +
 
 +
/* Gallery */
 +
.overlay ul li a::before{background-color:rgba(236, 255, 226, 0.6);}
 +
.overlay ul li a::after{color:#FFFFFF; }
 +
 
 +
/* */
 +
a:link {color: rgba(29, 5, 79, 0.81);}
 +
a:visited {color: rgba(29, 5, 79, 0.81);}
 +
a:hover {color:rgba(22, 19, 29, 0.95);}
 +
a:active {color:rgba(29, 5, 79, 0.81);}
 +
img
 +
{
 +
    filter: type(value);
 +
}
 +
img
 +
{
 +
    filter: type(value);
 +
    -webkit-filter: type(value);
 +
    -moz-filter: type(value);
 +
    -ms-filter: type(value);
 +
    -o-filter: type(value);
 +
}
 +
.grayscale img:hover
 +
{
 +
        filter: grayscale(0);
 +
        -webkit-filter: grayscale(0);
 +
        -moz-filter: grayscale(0);
 +
        -o-filter: grayscale(0);
 +
        -ms-filter: grayscale(0);
 +
}
 +
.grayscale img
 +
{
 +
        filter: grayscale(1);
 +
        -webkit-filter: grayscale(1);
 +
        -moz-filter: grayscale(1);
 +
        -o-filter: grayscale(1);
 +
        -ms-filter: grayscale(1);
 +
}
 +
 
 +
.subtitle{
 +
right: 5px;
 +
margin-top:30px;
 +
margin-bottom:-40px;
 +
}
 +
 
 +
.title h3{
 +
color:rgba(29, 5, 79, 1);
 +
font-size:25px;
 +
}
 +
 
 +
.subtitle h3{
 +
color:#90A83E;
 +
font-size:25px;
 +
}
 +
 
 +
 
 +
.title{
 +
font-size:25px;
 +
color:rgba(29, 5, 79, 0.81);
 +
}
 +
 
 +
.maintitle{
 +
 
 +
font-size:30px;
 +
color:rgba(29, 5, 79, 0.81);
 +
}
 +
 
 +
p.legend{
 +
font-size:78%;
 +
margin-bottom:20px;
 +
padding-bottom:10px;
 +
}
 +
 
 +
 
 +
table.team{
 +
text-align:center;
 +
background-color:rgb(0,0,0,0); /*transparent*/
 +
 
 +
}
 +
 
 +
table.team td {
 +
padding-top: 15px;
 +
padding-bottom: 15px;
 +
width:472px;
 +
height:327px;
 +
}
 +
 
 +
 
 +
 
 +
table.team td img{
 +
width:472px;
 +
height:327px;
 +
}
 +
 
 +
.anchor {
 +
    height: 30px;
 +
    position: fixed;
 +
    bottom: 30px;
 +
    right: 30px;
 +
    z-index: 1000;
 +
    cursor: pointer;
 +
    -webkit-transition: all ease 0.4s;
 +
    -moz-transition: all ease 0.4s;
 +
    -ms-transition: all ease 0.4s;
 +
    -o-transition: all ease 0.4s;
 +
    transition: all ease 0.4s;
 +
}
 +
/* SLIDESHOW */
 +
 
 +
    .contener_slideshow
 +
    {
 +
      width:800px;
 +
      height:600px;
 +
      overflow: hidden;
 +
      position: relative;
 +
      background-image: url(YOUR-URL-IMAGE.png);
 +
    }
 +
    .slid_1, .slid_2, .slid_3
 +
    {
 +
      position: absolute;
 +
      width:800px;
 +
      height:600px;
 +
    }
 +
    .slid_1{left: 0;}
 +
    .slid_2{left: 800px;}
 +
    .slid_3{left: 1600px;}
 +
    .contener_slide
 +
    {
 +
      width: 2400px;
 +
      height: 600px;
 +
      left:0px;
 +
      position: absolute;
 +
      -webkit-animation-duration: 10s;
 +
      -webkit-animation-iteration-count:infinite;
 +
      -webkit-animation-name: anim_slide;
 +
      -moz-animation-duration: 10s;
 +
      -moz-animation-iteration-count:infinite;
 +
      -moz-animation-name: anim_slide;
 +
      -ms-animation-duration: 10s;
 +
      -ms-animation-iteration-count:infinite;
 +
      -ms-animation-name: anim_slide;
 +
      animation-duration: 10s;
 +
      animation-iteration-count:infinite;
 +
      animation-name: anim_slide;
 +
    }
 +
    @-webkit-keyframes anim_slide
 +
    {
 +
      0% {left:0px;}
 +
      22% {left:0px;}
 +
      33% {left:-800px;}
 +
      45% {left:-800px;}
 +
      66% {left:-1600px;}
 +
      90% {left:-1600px;}
 +
    }
 +
    @-moz-keyframes anim_slide
 +
    {
 +
      0% {left:0px;}
 +
      22% {left:0px;}
 +
      33% {left:-800px;}
 +
      45% {left:-800px;}
 +
      66% {left:-1600px;}
 +
      90% {left:-1600px;}
 +
    }
 +
    @-ms-keyframes anim_slide
 +
    {
 +
      0% {left:0px;}
 +
      22% {left:0px;}
 +
      33% {left:-800px;}
 +
      45% {left:-800px;}
 +
      66% {left:-1600px;}
 +
      90% {left:-1600px;}
 +
    }
 +
    @keyframes anim_slide
 +
    {
 +
      0% {left:0px;}
 +
      22% {left:0px;}
 +
      33% {left:-800px;}
 +
      45% {left:-800px;}
 +
      66% {left:-1600px;}
 +
      90% {left:-1600px;}
 +
    }
 +
 
 +
</style>
 
</html>
 
</html>

Revision as of 12:29, 31 August 2015