Difference between revisions of "Team:NTNU Trondheim"

Line 1: Line 1:
 
{{NTNU_Trondheim_Draft}}
 
{{NTNU_Trondheim_Draft}}
 
<html>
 
<html>
</div>
 
 
</div>
 
</div>
 
</html>
 
</html>
Line 7: Line 6:
 
          <div class="banner">
 
          <div class="banner">
  
    </div>
 
 
     </div>
 
     </div>
  

Revision as of 20:08, 31 August 2015

Welcome to the NTNU Trondheim iGEM 2015 webpage!

NTNU Trondheim iGEM 2015

NTNU Trondheim is a team from Trondheim, Norway, competing for the iGEM 2015 student competition. NTNU Trondheim maintains the iGEM Matchmaker Tool which helps iGEM teams cooperate with each other.


We plan to engineer the soil bacterium Pseudomonas putida to function as a glucose detector. In the presence of glucose it will express the red fluorescent protein mCherry (see Figure 1). To achieve this we will use the promoters of four operons commonly found in Pseudomonas putida. The operons are negatively controlled, and the expression of subsequent genes is initiated by derivatives of glucose. We will characterize the performance of these four promoters and choose one to do further work on.

Further, we will encapsulate our engineered bacteria in alginate. This is a polymeric biomaterial from brown algae that can be used to form gel capsules. By encapsulating living cells in alginate, the semi permeable gel capsule will shield the cells from the surroundings at the same time as letting small molecules like glucose in and out (Figure 1).


Glucose metabolism
Figure 1 - Alginate encapsulated Pseudomonas putida will express the red fluorescent protein mCherry upon glucose detection.

Read more...