Difference between revisions of "Team:Aalto-Helsinki/InterLab"

m (added lab book link)
(Added data into tables)
Line 37: Line 37:
  
 
<h2>Results</h2>
 
<h2>Results</h2>
 +
 +
<h4>1st Technical Replicon </h4>
  
 
<table class="table table-bordered">
 
<table class="table table-bordered">
Line 47: Line 49:
 
       <td><h4 style="margin-top:0;">Empty backbone</h4></td>
 
       <td><h4 style="margin-top:0;">Empty backbone</h4></td>
 
       <td><h4 style="margin-top:0;">Without backbone</h4></td>
 
       <td><h4 style="margin-top:0;">Without backbone</h4></td>
−
       <td><h4 style="margin-top:0;">Blank</h4></td>
+
       <td><h4 style="margin-top:0;">Blanks</h4></td>
 
     </tr>
 
     </tr>
 
   </thead>
 
   </thead>
Line 53: Line 55:
 
     <tr>
 
     <tr>
 
       <td><p>1</p></td>
 
       <td><p>1</p></td>
−
       <td><p>D1</p></td>
+
       <td><p>OVRFLW</p></td>
−
       <td><p>D2</p></td>
+
       <td><p>83954</p></td>
−
       <td><p>C1</p></td>
+
       <td><p>53925</p></td>
−
       <td><p>C2</p></td>
+
       <td><p>16309</p></td>
−
       <td><p>C3</p></td>
+
       <td><p>13663</p></td>
−
       <td><p>B</p></td>
+
       <td><p>29336</p></td>
 
     </tr>
 
     </tr>
 +
    <tr>
 +
      <td><p>2</p></td>
 +
      <td><p>OVRFLW</p></td>
 +
      <td><p>OVRFLW</p></td>
 +
      <td><p>56764</p></td>
 +
      <td><p>16335</p></td>
 +
      <td><p>14148</p></td>
 +
      <td><p>14940</p></td>
 +
    </tr>
 +
      <td><p>3</p></td>
 +
      <td><p>OVRFLW</p></td>
 +
      <td><p>OVRFLW</p></td>
 +
      <td><p>61918</p></td>
 +
      <td><p>16771</p></td>
 +
      <td><p>14073</p></td>
 +
      <td><p> </p></td>
 +
  </tbody>
 +
</table>
 +
 +
<h4>2nd Technical Replicon </h4>
 +
 +
<table class="table table-bordered">
 +
  <thead>
 +
    <tr>
 +
      <td><h4 style="margin-top:0;">Sample</h4></td>
 +
      <td><h4 style="margin-top:0;">J23117 + I13504</h4></td>
 +
      <td><h4 style="margin-top:0;">J23101 + I13504 </h4></td>
 +
      <td><h4 style="margin-top:0;">J23151</h4></td>
 +
      <td><h4 style="margin-top:0;">Empty backbone</h4></td>
 +
      <td><h4 style="margin-top:0;">Without backbone</h4></td>
 +
      <td><h4 style="margin-top:0;">Blanks</h4></td>
 +
    </tr>
 +
  </thead>
 +
  <tbody>
 
     <tr>
 
     <tr>
 
       <td><p>1</p></td>
 
       <td><p>1</p></td>
−
       <td><p>D1</p></td>
+
       <td><p>OVRFLW</p></td>
−
       <td><p>D2</p></td>
+
       <td><p>85224</p></td>
−
       <td><p>C1</p></td>
+
       <td><p>53553</p></td>
−
       <td><p>C2</p></td>
+
       <td><p>16307</p></td>
−
       <td><p>C3</p></td>
+
       <td><p>13747</p></td>
−
       <td><p>B</p></td>
+
       <td><p>29470</p></td>
 
     </tr>
 
     </tr>
 +
    <tr>
 +
      <td><p>2</p></td>
 +
      <td><p>OVRFLW</p></td>
 +
      <td><p>OVRFLW</p></td>
 +
      <td><p>56472</p></td>
 +
      <td><p>16204</p></td>
 +
      <td><p>14267</p></td>
 +
      <td><p>14972</p></td>
 +
    </tr>
 +
      <td><p>3</p></td>
 +
      <td><p>OVRFLW</p></td>
 +
      <td><p>OVRFLW</p></td>
 +
      <td><p>60969</p></td>
 +
      <td><p>16818</p></td>
 +
      <td><p>14381</p></td>
 +
      <td><p> </p></td>
 +
  </tbody>
 +
</table>
 +
 +
<h4>3rd Technical Replicon </h4>
 +
 +
<table class="table table-bordered">
 +
  <thead>
 +
    <tr>
 +
      <td><h4 style="margin-top:0;">Sample</h4></td>
 +
      <td><h4 style="margin-top:0;">J23117 + I13504</h4></td>
 +
      <td><h4 style="margin-top:0;">J23101 + I13504 </h4></td>
 +
      <td><h4 style="margin-top:0;">J23151</h4></td>
 +
      <td><h4 style="margin-top:0;">Empty backbone</h4></td>
 +
      <td><h4 style="margin-top:0;">Without backbone</h4></td>
 +
      <td><h4 style="margin-top:0;">Blanks</h4></td>
 +
    </tr>
 +
  </thead>
 +
  <tbody>
 +
    <tr>
 
       <td><p>1</p></td>
 
       <td><p>1</p></td>
−
       <td><p>D1</p></td>
+
       <td><p>OVRFLW</p></td>
−
       <td><p>D2</p></td>
+
       <td><p>86750</p></td>
−
       <td><p>C1</p></td>
+
       <td><p>56390</p></td>
−
       <td><p>C2</p></td>
+
       <td><p>16123</p></td>
−
       <td><p>C3</p></td>
+
       <td><p>13536</p></td>
−
       <td><p>B</p></td>
+
      <td><p>30778</p></td>
 +
    </tr>
 +
    <tr>
 +
      <td><p>2</p></td>
 +
      <td><p>OVRFLW</p></td>
 +
      <td><p>OVRFLW</p></td>
 +
      <td><p>58294</p></td>
 +
      <td><p>16294</p></td>
 +
      <td><p>14207</p></td>
 +
      <td><p>14856</p></td>
 +
    </tr>
 +
      <td><p>3</p></td>
 +
      <td><p>OVRFLW</p></td>
 +
      <td><p>OVRFLW</p></td>
 +
      <td><p>62229</p></td>
 +
      <td><p>16793</p></td>
 +
      <td><p>13994</p></td>
 +
       <td><p> </p></td>
 
   </tbody>
 
   </tbody>
 
</table>
 
</table>

Revision as of 11:06, 3 September 2015

InterLab Study

Our InterLab Study Lab Book can be found here for a fully detailed overview of our study.

Devices

The following devices were created in a backbone pSB1C3:

⚫ J23101 + I13504 (B0034-E0040-B0015)
⚫ J23106 + I13504 (B0034-E0040-B0015).

However, constructing a device J23117 + I13504 (B0034-E0040-B0015) wasn't successful due to the time limit and limited ligation times. More details available on Lab Book. The device sizes were analyzed with restriction mapping which results can be seen in Figure 1.

Used BBa_I20270, a GFP expressing part in the pSB1C3 backbone as a positive control. Negative controls were organisms with an empty plasmid and without any vector.

Protocols

Constructing devices

Protocols for the restriction, ligation and transformation of BioBricks can be found here.

Cultivations and measurement

Followed the next protocol for preparing the samples:
Streaked out LB-plates with E.coli TOP10 strain containing every device and control with chloramphenicol concentration of 35ug/ml. Incubated plates overnight (18-20 hours) at 37C. Picked up biological triplates from the plates and inoculated 3 ml liquid cultures from the colonies with 12ml polypropylene test tube. Incubated the tubes at 37C with shaking at 300rpm for 16-18 hours.

Results

1st Technical Replicon

Sample

J23117 + I13504

J23101 + I13504

J23151

Empty backbone

Without backbone

Blanks

1

OVRFLW

83954

53925

16309

13663

29336

2

OVRFLW

OVRFLW

56764

16335

14148

14940

3

OVRFLW

OVRFLW

61918

16771

14073

2nd Technical Replicon

Sample

J23117 + I13504

J23101 + I13504

J23151

Empty backbone

Without backbone

Blanks

1

OVRFLW

85224

53553

16307

13747

29470

2

OVRFLW

OVRFLW

56472

16204

14267

14972

3

OVRFLW

OVRFLW

60969

16818

14381

3rd Technical Replicon

Sample

J23117 + I13504

J23101 + I13504

J23151

Empty backbone

Without backbone

Blanks

1

OVRFLW

86750

56390

16123

13536

30778

2

OVRFLW

OVRFLW

58294

16294

14207

14856

3

OVRFLW

OVRFLW

62229

16793

13994