Difference between revisions of "Team:Czech Republic/Interlab study"

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= Design =
 
= Design =
 +
 +
=Materials and methods=
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==Used plasmids==
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1. Device 1 (20K+21J): J23101 + I13504, in the pSB1C3 backbone
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2. Device 2 (22A+21J): J23106 + I13504, in the pSB1C3 backbone
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3. Device 3 (22K+21J): J23117 + I13504, in the pSB1C3 backbone
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4. Positive control (C+): I20270,  in the pSB1C3 backbone
 +
5. Negative control (C-): K823008, in the pSB1C3 backbone
 +
  
  

Revision as of 14:40, 3 September 2015

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Background

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Design

Materials and methods

Used plasmids

1. Device 1 (20K+21J): J23101 + I13504, in the pSB1C3 backbone 2. Device 2 (22A+21J): J23106 + I13504, in the pSB1C3 backbone 3. Device 3 (22K+21J): J23117 + I13504, in the pSB1C3 backbone 4. Positive control (C+): I20270, in the pSB1C3 backbone 5. Negative control (C-): K823008, in the pSB1C3 backbone


Protocols

Device Colony MEFL Mean of technical replicates SD Colony Mean of device SD Device
20K +21J (Device 1) Colony 1 I. 639549 657818 15504 664409 29185
II. 677453
III. 656453
Colony 2 I. 689717 702990 10067
II. 714084
III. 705169
Colony 3 I. 620331 632419 15053
II. 623286
III. 653639
22A+21J (Device 2) Colony 1 I. 481600 464304 12795 392603 50712
II. 460265
III. 451048
Colony 2 I. 346283 358070 9696
II. 357896
III. 370032
Colony 3 I. 354544 355433 4688
II. 350188
III. 361568
22K+21J (Device 3) Colony 1 I. 2635 2872 220 2229 454
II. 3165
III. 2814
Colony 2 I. 1786 1891 74
II. 1933
III. 1953
Colony 3 I. 1665 1926 204
II. 1948
III. 2163
Positive control Colony 1 I. 202361 196401 5853 196401 /
II. 188446
III. 198395
Negative control Colony 1 I. 224 213 8 213 /
II. 204
III. 211


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